PK X֒|ZZdocProps/core.xml PK Xw,,docProps/app.xml SheetJSWorksheets1PK X}f}fxl/worksheets/sheet1.xml 年度計畫名稱_中文計畫名稱_英文計畫編號_中文計畫編號_英文主辦單位_中文主辦單位_英文主辦人_中文主辦人_英文中文摘要英文摘要2013 組織培養節能設備及技術之開發 Energy-saving equipment and technology development for tissue culture 102農科-14.1.6-種-X1 102AS -14.1.6-SS-X1 繁殖技術課 Propagation Technology Section 文紀鑾 Chi-Luan Wen 不公開 Not public2013 健康種苗生產供應與健康管理體系之建立 Establishment of health seedlings production and health management system 102農科-14.2.2-種-X1 102AS -14.2.2-SS-X1 繁殖技術課 Propagation Technology Section 羅英妃 Ying-Fei Lo 一般認為芽菜為無土栽培之清潔蔬菜，但由於芽菜為時間與空間高密集周轉生產，故生產過程為抑菌、殺菌、促進下胚軸肥胖及抑制過多主根及鬚根生長，往往添加植物生長調節劑、漂白劑等，以致目前仍無相關符合生產履歷措施之作業與產品。本計畫調查台灣地區傳統市場、超級市場或量販店銷售之綠豆芽或黃豆芽，結果仍有二氧化硫、6-BA或24-D等添加物質之檢出。本計畫調查市售綠豆真菌汙染率為42% ，細菌汙染率為24%，以 70℃熱處理 30分鐘即可有效將綠豆種子的真菌汙染率下降至為25% ，細菌汙染率為5% ，並且不會影響種子之發芽率。後續配合業界將自有技術如芽菜加壓生產栽培箱，芽菜根部截切機械原型，建立無施用藥劑之芽菜安全生產流程，成功輔導1家豆芽生產安全無用藥廠商。完成馬鈴薯主要產區瘡痂病調查，食用薯調查結果顯示，使用通過驗證種薯田區瘡痂病發生情況減輕約55%，顯示使用健康種薯有助於降低瘡痂病害之威脅。另以非農藥防治管理栽培生產馬鈴薯種薯，種薯產量與品質與傳統栽培無差異，可減少化學藥劑使用之風險，栽培時建議將30-10-10肥料稀釋1000倍使用，以確保種薯產量與品質。本次蒐集之7個豇豆品種實生苗經ELISA病毒篩選，復於隔離網室栽培後，各品種間種子產量上似乎存有明顯差異。選擇萎凋病發生嚴重的產地田區進行嫁接苗耐病性測試，以直播苗為對照組，在田區第四次連作時，直播苗全數萎凋死亡，存活率0%，嫁接苗部分則仍有80%的存活率，產量部分，直播苗全數萎凋死亡，產量0%，嫁接苗部分則仍有665公斤/0.1ha的收成，顯示耐病根砧可以提高田間存活率並穩定產量。 This project investigated green bean or soybean sprouts bought fromtraditional market or supermarkets in Taiwan. Some added substances such as sulfur dioxide6-BA or 24-D were detected. We also found out the rate of fungal and bacterial contamination of commercially available green bean seeds were 42% and 24% respectively. After 30 minutes heat treatment at 70℃ the contaminated seeds changed into clean effectively and did not affect the germination. We established a safe sprouts production processes by growth enviroment control and sprouts cultivated pressurized containers and sprouts rootscutting machine. This project advised a professional grower to produce safe sprouts without chemical or plant growth regulator. According to our survey at major potato production areas in Taiwan common scab disease was decreased 55% by using certified seed potato. It shows that using healthy seed potato can avoid the risk of common scab disease. Compared non-pesticidal and non-fungicidial management with traditional management the yield and quality of seed potato were no significant difference. The result shows that we can reduce the risk of using chemistry by non-pesticidal and non-fungicidial management. And we also recommend using 1000x the period of cultivation to get better seed potato.The results of preliminary experience show that the virus-free seedlings of 7 Asparagus been varieties planting in net house there seemed significant differences in seed yield.sparagus bean (yard-long bean) varieties and one fusarium-resistant stock were tested in the study to evaluate the resistance of fusarium wilt in the field.The results showed of plant survival rate of seed seedlings and grafted asparagus bean planted in production field at 4th continuous cropping were 0% and 82% respectively. The yield were 0 kg and 655 kg/ 0.1 harespectively.2014 基因轉殖及非基因轉殖種苗驗證及共存體系之建構-基因轉殖作物認驗證與檢監測模式之建立 Construction of the certification and coexistence system for GM and non-GM seedlings 103農科-1.1.1-種-X1 103AS -1.1.1-SS-X1 生物技術課 Biotechnology Section 陳哲仁 Jen-Ren Chen 基因轉殖作物檢監測體系由農糧署委託種苗改良繁殖場邀集農業試驗所、桃園區農業改良場、台南區農業改良場、花蓮區農業改良場、國立中興大學等單位建立「基因轉殖作物檢測監測小組」。持續檢測木瓜、玉米、大豆、水稻、馬鈴薯、油菜等栽培區及進口飼料，蒐集基改作物標準樣品並開發相應檢測技術。種苗改良繁殖場基因轉殖作物檢測實驗室已取得「財團法人全國認證基金會」之生物領域測試實驗室認證。本場今年支援花東地區檢測45件及進口飼料 34件，並完成基改玉米共存模式建議書及「基因轉殖作物混雜度調查資料管理系統」地理資訊系統。 "The establishment of the surveillance platform in Taiwan was entrusted by the Agriculture and Food Agency (AFA) Council of Agriculture (COA). Teamwork members are composed of Seed Improvement and Propagation Station(SIPS) Agricultural Research Institute Taoyuan Tainan and Hualien District Agricultural Research and Extension Station and National Chung Hsing University and constantly monitoring papaya maize soybeanrice potato and rape seed production farms and imported feed ingredients. We consistently collect reference materials of GM crops and the development of the corresponding detection techniques. The biological area of GMO detection laboratory at SIPS has been accredited by Taiwan Accreditation Foundation (TAF).This year we supporting survey task of 45 GM crops of eastern Taiwan and 34 feed ingredients from border customs. And proposed GM mazie coexistence model recommendation and “GM crop Survey Data Management System"" geographic information systems to duty authorities."2014 生技種苗檢測服務建置與產業推動 Industry extension and establishment ofbiotech seedling detection serviceplant seed/seedling team 103農科-1.1.2-種-X1 103AS -1.1.2-SS-X1 生物技術課 Biotechnology Section 鍾文全 Wen-Chuan Chung 利用已開發共顯性分子標誌(Ty1 Ty2及Ty3)，檢測種苗業界所提供的番茄種苗是否具有抗黃化捲葉病毒植株，得知三家種苗業者所育出的番茄種苗主要以抗Ty2為主。文心蘭檸檬綠組培苗依株高等級分為小苗、中苗、大苗等三級，評估小瓶苗要達到出貨標準(株高達7.4cm以上 )約需培養55天，大瓶苗培養30天即可出貨。拜訪6家仙履蘭組培場，與經營者訪談組培設備、生產流程及瓶苗出貨標準，目前仙履蘭組培瓶苗大多為實生苗，由栽培者提供種子交由專業組培場代工。以栽培場自行雜交之綠Maudiae 品種，無菌播種之實生瓶苗為試驗材料，培養3、4、6個月後，得知仙履蘭瓶苗應以培養4個月後為出瓶最佳時機。本年度蒐集四個流通品種(Phal. TailinRed Angle ‘V31’、Phal. I-Hsin White Swan、Dtps. Tinny Honey、Dtsp. IHsin Yellow Tris)之蝴蝶蘭組培瓶苗，進行生長指數調查與植體營養成分分析，並將蝴蝶蘭瓶苗依102年計畫建立之瓶苗品質鑑定指標進行分級種植，四個流通品種進行瓶苗形態調查及植體分析，並發育到中苗，調查花形間品質指標之結果驗證，結果顯示 5個月後植株之乾鮮重、乾物重、葉幅、葉數、根數等四個品種各級間皆無顯著性差異，若從元素分析來看，各級間元素佔植株乾重的百分比亦並無顯著性差異，但不合苗之單株全可溶性糖含量之表現與合格苗差異較其他元素更為明顯。以去病毒技術篩選蘭園蝴蝶蘭無病毒之母本，共篩選500件瓶苗樣品，結果得知獲得去病毒的比率會隨寄主種類與感染病毒嚴重度有所差異，獲得去病毒的健康蝴蝶蘭種苗比例介於0-15.3%之間。 Tomato seedlings provided by seed industry were detected through molecular markers (Ty-1 Ty-2 and Ty-3 developed by TSIP) for identifying TYLCV-ressitant line or variety. Results showed that all tested belong to TY-2 line. Oncidium plants are classified into three levels. To reach standard shipping for small seedling it could grow up to 55 dayswhereas large seedlings only were 30 days. To understand the company profile production processes and plantlets standards in this year we visit six paphiopedilum tissue culture factories. Leave number root number plant height root length fresh/dry weight of paphiopedilum TCplantlets were studied 32014 種子檢查技術研習 Study on seed testing technology 103農科-4.2.1-種-X1 103AS -4.2.1-SS-X1 種苗經營課 Seed & Seedling Management Section 許鐈云 Ciao-Yun Syu 本年度參與ISTA於2014年06月23～26日在英國愛丁堡(Edinburgh Scotland U K ) 舉行由蘇格蘭農業科學策進會( S c i e n c e a n d A d v i c e f o r S c o t t i s h Agriculture SASA)所承辦種子取樣及品質保證研習(Seed Sampling and Quality Assurance in Seed Sampling)。參與研習的學員各來自法國、拉脫維亞、瑞典、德 國、荷蘭、南韓、中國大陸、波蘭、美國、丹麥、比利時及台灣等12個國家。研習課程內容主要包含：種子取樣的原理及方法、取樣儀器介紹、取樣的品質保證及取樣員的監測等，同時並參訪ISTA認證的實驗室。在報告中也提出改善建議，如：改正取樣技術、建立取樣員的訓練方法及考核、取樣品質管理的改善等。 In 2014 one staff of TSS attended the ISTA workshop on seed sampling and quality assurance of seed sampling on 23-26 June 2014 in United Kingdom . The workshop was hold by Science and Advice for Scottish Agriculture (SASA) (ISTA Accredited Code: GB04) in Edinburgh. The participators in this workshop came from France Latvia Sweden Germany Netherlands Korea China2014 赴荷蘭建置雙方蝴蝶蘭DNA資料庫 Established the Phalaenopsis DNA database of both sides in Netherlands 103農科-4.2.1-種-X2 103AS -4.2.1-SS-X2 生物技術課 Biotechnology Section 張惠如 Hui-Ju Chang Naktuinbouw為荷蘭執行植物品種檢定的專責機構，亦負責歐盟成員國數種作物品種申請案件之檢定工作，具相當豐富的品種檢定業務經驗及人力。本計畫前往Naktuinbouw進行14天的研習，主要的研習內容為利用BioNumerics分析軟體進行蝴蝶蘭品種基因型分析，建立蝴蝶蘭DNA資料庫。經過多次演練並和Naktuinbouw技術人員的經驗交流後，協助我方建立蝴蝶蘭DNA資料庫時需注意的參數設定及資料格式，並且解決未來雙方合作所需交換的資料內容等問題。為有效辦理第14屆臺荷農業合作會議之決議事項，針對雙方技術合作案，在交換蝴蝶蘭DNA材料與基因型分析資料的工作上，雙方取得共識並撰寫相關細節內容文件一份。除此之外，在研習期間也參加了Naktuinbouw針對育種者及業者所舉辦的說明會，會中共有四個議題，內容主要講述有關DNA分子標誌使用於性狀檢定及侵權鑑定工作上所扮演的角色。在這次研習後，雙方基於這些討論與交流，在建立蝴蝶蘭品種DNA資料庫這項工作上，將在技術操作的細節上更加留意。未來工作將著重在加強與性狀檢定技術人員、育種者及業者溝通交流。另外，進一步將DNA資料庫與性狀資料庫及影像資料庫進行連結，以提升分子鑑別技術及DNA資料庫於蝴蝶蘭性狀檢定作業上的輔助性。 Naktuinbouw in the Netherlands is the organization authorized to assess varieties of agricultural floricultural arboricultural and vegetable crops for distinctness uniformity and stability (DUS testing) for registration purposes and/or granting Plant Breeders' Rights both on Dutch an EU level. The objectives of this study within 2 weeks in Naktuinbouw were to learn genotyping on Phalaenopsis varieties by BioNumerics software and to establish the DNA database of Phalaenopsis varieties. Both techniques and experiences through this study could not only establish the methods (such as parameter data file format ... etc)but also solve problems of both sides cooperation. To effectively handle the issues of both sides cooperation in 14th Taiwan-Netherlands Agricultural Working Group Meeting the researchers of Taiwan TSIPS and Naktuinbouw had written the document “Proposal of the cooperation between TSIPS and Naktuinbouw” including exchange works of DNA materials and analysis results. Meanwhile I also attended the information meeting for breeder and seed company during this study. Four programs were conducted in this meeting mainly focusing on the role of DNA makers in the DUS testing and against infringement on Plant Breeders’ rights.After this study both sides pay more attention on detail technical operations of DNA database of Phalaenopsis species based on those discussions and experiences. In the future works2014 活化農地計畫之重要農產品供應鏈加值整合策略研究(種子供應鏈研究) A Study on Value-added Strategies of Supply Chain of Important Agricultural Products Base on Arable Land Activation Program. 103農科-5.1.6-種-X1 103AS -5.1.6-SS-X1 技術服務室 Technical Service Section 周明燕 Ming-Yenn Chou 蔬菜種子產業供應鏈包含種原、育種、採種、銷售及生產栽培。規模較大的農企業已有能力自行建構完整供應鏈，而供應鏈中的各環節也可透過小型農戶所自營的種苗圃、育苗場、種子行等進行委外採種、育苗、銷售等工作。此外，國際種子公司也透過代理、經銷模式進入我國市場。種苗產業供應鏈中核心關鍵在研發端及通路端。因此，蔬菜種子產業供應鏈加值改善可以從幾個點作提升：在研發端，建議由產學研籌組共同研發平台，共享資源；在種子生產部份，宜提升種子生產效能，導入精準生產管理模式以克服生產成本高漲困境；在中間物流部分，宜積極與通路業者建立夥伴關係，提高彼此互信及依存黏度，共榮共享；消費端則可以透過共同參展行銷，互相拉抬，建立臺灣優質品牌形象。蔬菜種子外銷前景廣受看好，受限於採種大環境，在國內設置採種基地的可行性並不高。但業者為了避免親本外流，研發工作及親本採種作業仍傾向於在臺灣進行，研發及親本乃是種子公司的核心資產，雖然需要的土地不高，卻能創造高額利潤，因此，政府應積極協助業者取得適當的休耕農地，建置研發中心，讓臺灣成為國際蔬菜品種研發重鎮，對整體產業發展的助益將是迅速且直接。 Vegetable seed industry supply chain that contains the collection of the original species breeding seed production sale and production of cultivation. Large-scale agricultural enterprises have the ability to self-construct the complete supply chain. Each link in the supply chain can also be carried out through the link to complete outsourcing seedbreeding sales and so on. In addition international seed companies have entered the Taiwan market through an agent distribution patterns. R ＆ D and critical path is the core plant seed industry supply chain. Improve value-added vegetable seed industry can improve supply chain bonus from several points: In the R ＆ D side it is recommended by industryacademia and research departments jointly developed platform to share resources; Part in seed production improve production efficiency seeds imported by the quasi-production management2014 青年農民植物種苗類別訓練成效追蹤評核之研究 The effectiveness assessment researchof category training in seedling for young farmers 103農科-5.2.1-種-X1 103AS -5.2.1-SS-X1 技術服務室 Technical Service Section 鍾依萍 I-Ping Chung 「國家發展計畫(102-105年度)有關農業政策的施政重點之一「調整農業結構，培育農業人才，整合資源加值發展」，農業的未來須朝向人力年輕化、休耕農地活化、推動農業教育訓練與後續輔導服務。種苗改良繁殖場加入農委會輔導處「提升青年農民訓練成效之研究」共同研究主題計畫，針對農民學院植物種苗類別訓練課程進行結訓學員的訓練成效追縱評核，藉以了解學員參加農民學院後之效果或變化，並建構適合青年從農訓練模式。 The aim of the investigation is to evaluate the trainees who and had completed the plant seeding training courses from the level of basic and the level of intermediate(plant tissue culture technology training) in the farmers’ academy during the years 2011 to 2013. Investigation 1.Basic information 2. Training performance evaluation through mailed questionnaires.2014 木瓜種子檢查技術產業連結與國際接軌 Seed Testing Industry Connection and International Corparation of Papaya Seed 103農科-6.2.3-種-X1 103AS -6.2.3-SS-X1 種苗經營課 Seed & Seedling Management Section 沈翰祖 Han-Tsu Shen 不公開 Not public2014 基因轉殖棉花、番茄、小麥檢測技術之研究 Studies of transgenic cotton tomato and wheat detection echnology 103農科-6.2.3-種-X2 103AS -6.2.3-SS-X2 生物技術課 Biotechnology Section 張惠如 Hui-Ju Chang 為建立轉殖基因棉花的檢測作業標準流程，先進行不同核酸萃取方法及棉花種子萃取前處裡的試驗，依結果並考量檢測流程的效率與成本，採用先利用均質機將棉花種子處理15秒後，再將種殼與種仁一起以自動萃取方式來進行棉花種子樣品之核酸萃取方法。透過此方法獲得的DNA溶液以歐盟公告之檢測方法進行轉殖品項PCR定性檢測試驗，結果顯示在不同轉殖品項LLCOTTON 25、GHB614、GHB119、T304-40中，可各自得到預期79 bp、119 bp、90 bp、78 bp大小的目標片段。經過多次確認試驗後，完成建立檢測轉殖品項LLCOTTON 25及GHB614之標準作業流程。 In this study we used different methods for transgenic cotton seed DNA extraction. According to DNA quality and considered the efficiency and cost of testing process we selected the homogenizer to pretreatment 15 seconds then extracted DNA from cotton seed by automated method (Smart LabAsist-16). DNA solution is obtained by this method and used as template DNA in PCR reaction for qualitative detection. The PCR condition was follow the European Union reference reports. Results are displayed in different specific transgenic event consist of LLCOTTON 25 GHB614GHB119 T304-40 each of them can be get the expected target fragment size as 79 bp 119 bp 90 bp 78 bp. After several confirm tests2014 國外農企業基因轉殖作物商業品種檢測技術之建立 Establishment of GM crops detection technology for foreign commercial varieties 103農科-6.2.3-種-X3 103AS -6.2.3-SS-X3 生物技術課 Biotechnology Section 陳哲仁 Jen-Ren Chen 基因轉殖作物在國際市場上規模日益增加的情形，帶動建立相應檢測評估基因轉殖作物存在技術的強烈需求，本計畫的主要目的是建立一套基因轉殖作物標準檢測技術，完成玉米19項、大豆9項以及油菜3項基改品項檢測技術，提供主管機關執行進口飼料基因轉殖作物邊境查驗與管理。 The increasing presence of transgenic plant in the international markets has provoked a strong demand for appropriate detection methods to evaluate the existence of transgenic plants in the field and feed ingredients. The major objective of this study was to establish a detection technique in GM maize soybean and rapeseed. We were established 19 mazie 9 soybean and 3 rapeseed GM event detection methods and would achieve border control and inspection of imported GM feed ingredients for administration office.2014 中藥材流蘇石斛及種苗金童一號石斛組織培養健康種苗生產技術開發 Study on in vitro mass production technology of Dendrobium fimbriatummedicinal functions of Dendrobium Cassiope Taiseed Golden Boy No.1 103農科-6.2.3-種-X4 103AS -6.2.3-SS-X4 繁殖技術課 Propagation Technology Section 張珈錡 Jia-Ci Chang 1.本計畫嘗試建立流蘇石斛(Dendrobium fimbriatum)之組織培養繁殖技術、種苗生產模式，經試驗顯示流蘇石斛組培苗消毒以使用1%、0.5%次氯酸鈉進行2階段消毒，培植體之存活率最高達50%。芽體增殖階段以培養於DF2培養基2個月，植株之株高可達3.43cm、每芽有4.4片葉、每培殖體形成2.4芽、叢芽率34.68%為最低、培殖體褐化率僅3.00%較佳。發根階段則以培養於1402培養基，可獲得地上部和地下部表現較佳之植株。在種苗生產模式方面，初步探討不同種植芽數、出瓶時間對流蘇石斛組培苗生長之影響，由試驗結果得知，不同種植芽數與出瓶時間對於栽培4個月內之種苗並無顯著之影響。另外，本試驗觀察流蘇石斛在1年的栽培期間之生育變化，將有助於後續調整種苗栽培管理之模式。2.金童石斛（Dendrobium Cassiope‘Taiseed Golden boy No.1’）為蘭科（Orchidaceae）植物。金童石斛花開花時期為每年的2-4月，我們先前的研究發現金童石斛萃取物有豐富的酚類化合物，且展現優異的抗氧化效果。因此，本研究進一步以脂多醣 (lipopolysaccharide LPS) 誘發RAW264.7細胞株之發炎模式探討金童石斛乙醇萃取物的抗發炎功效，研究結果發現此萃取物具有抑制RAW264.7細胞發炎之抑制白細胞介素-6 (interleukin-6 IL-6)及腫瘤致死因子(tumor necrosis factor-α TNF-α)生成，進而抑制一氧化氮 (nitric oxideNO)和前列腺素E2 (prostagladin E2 PGE2)的生 1. This study attempts to establish a micropropagation method and seedlingproduction model of Dendrobium fimbriatum the test showed explants disinfected with 1% and 0.5% sodium hypochlorite twice the survival rate achieve 50 %. An shoot proliferation medium with code name DF2 was the best for shoot proliferationthe plant height up to 3.43 cm formed 2.4 buds per explants2012 植物種苗團隊-作物特定性狀分子標誌建立及選殖 The establishment and application of specific molecular markers for the crops trait 101農科-9.1.1-種-X1 101AS -9.1.1-SS-X1 生物技術課 Biotechnology Section 孫永偉 Yung-Wei Sun a.番茄抗斑點萎凋病毒基因型之分子鑑定：番茄斑點萎凋病毒病，為番茄溫室栽培的主要病害之一，近年更有蔓延的趨勢。危害症狀變化大，從苗期到成株各部位均可感染呈現症狀，Sw5抗性基因控制下接種TSWV的反應呈現無病斑或僅限制性過敏反應。本試驗完成在Sw5基因位置上建立分子標誌並建立Multiplex RT-PCR (含TSWV/Sw5)可同時檢定番茄抗斑點萎凋病毒病的同質抗性、異質抗性或感病性及是否帶有TSWV的病毒。利用F2族群分析抗性基因之分離情形，結果栽培種中之Sw5a與Sw5b抗性基因表現在栽培種中呈現不分離情形，分離比為1:2:1並已運用於大量樣品基因型檢定。利用ELISA及RT-PCR檢測接種TSWV病原植株，以檢定番茄抗斑點萎凋病毒病的同質抗性、異質抗性或感病性的抗病能力，結果同質抗性、異質抗性或感病性檢出病原的比例分別為0%、1.26%及41%。b.木瓜性別相關基因及其分子標誌之建立：由DNA層次進行分子標誌的篩選工作，希望能獲得與木瓜全兩性性狀相關之分子標誌。經過多組的SSR及SCAR引子PCR試驗篩選，電泳分析發現：目前所設計之引子對在各試驗材料間，在全兩性株及一般兩性株材料間，並無篩選出具可鑑別全兩性性狀之差異性條帶。進一步將增幅出的條帶進行序列分析，由序列上發現全兩性株與一般兩性株/雄株之間，同個增幅條帶內具有單個核甘酸的差異。這些單個核甘酸的差異是否可發展為SNP或CAPS分子標誌，而應用於全兩性性狀的判定，則需再進行試驗進行篩選測試後，方能由實驗結果來印證。c. 番茄抗黃化捲葉病毒分子標誌之建立：番茄黃化捲葉病毒(Tomato yellow leaf curl virusTYLCV)是由粉蝨傳播的嚴重病害，台灣主要有2種，ToLCTWV (mono-partite，台灣種) 及TYLCTHV(bi-partite，泰國種)。本研究與亞蔬中心合作利用不同育種者之抗感病番茄品種為試驗材料，主要目的在建立黃化捲葉病毒及番茄抗病基因(Ty-3a、Ty-5)分子標誌。結果顯示，引子BGVs可擴增Begomovirus屬病毒(含ToLCTWV及TYLCTHV) 1.6 kb之DNA條帶，引子TY-TH與TY-TW可分別擴增TYLCTHV與ToLCTWV病毒1.6 kb之DNA條帶。近年致病力較強之泰國種病毒發生率呈明顯上升趨勢。已建立抗病基因Ty-3a及Ty-5之SCAR分子標誌，引子Ty3可分別擴增抗病Ty-3、Ty-3a基因320、500及感病ty-3基因250 bp之DNA條帶，引子Ty5可擴增抗病Ty-5及感病ty-5基因750及650 bp之DNA條帶。上述分子標誌可協助育種者早期篩選抗病植株、確認抗病基因型(R/R、R/S、S/S)及病毒感染情形，提高育種效率。 The projects of this program have three components including. The first is the establishment and application molecular markers of the resistance to sport wilt virus in tomato analysis system. Specific markers SCAR-Sw5-1313 and SCAR-sw 5-676 were developed for differentiating allelic variation at Sw5 locus. It amplifies fragments from both resistant and susceptible tomato accessions with different molecular sizes that corresponding to the presence of Sw5/Sw5 Sw5/sw5 and sw5/sw5 alleles.This year we also merged SCAR-Sw 5-1313 SCAR-sw 5-676 and TSWV as Multiplex-PCR through once RT-PCR detecting Sw5/Sw5 Sw5/sw5 sw5/sw5 and TSWV simultaneously. Sw5a and Sw5b are resistance genes to sport wiltvirus in chromosome 9 of tomato. Genes (Sw 5a and Sw 5b) could beidentified by molecular markers Tss#Sw5a and Tss#Sw5b respectively. F2 segregation rate of Sw5aSw5b /Sw5aSw5b Sw5aSw5b/sw5 and sw5/sw5 was 1:2:1. Inoculation experiment of TSWV to 2 F2 populations showed superiority of homo-resistance genotype and hetero-resistance genotype to susceptibility genotype.The second is establishment the molecular markers for sex determination in Carica papaya L. (papaya) by using SSR and SCAR analysis. In SSR-PCR and SCAR-PCR analysis we didn't found the maker was specific to allhermaphrodite character. Most of these primers can get a band in malehermaphrodite and all-hermaphrodite materials. The sequence analysis results of the band were showed single nucleotide differences in male/hermaphrodite or hermaphrodite/ all-hermaphrodite materials. These single nucleotide differences might be applied to SNP or CAPS molecular markers which can detected the all-hermaphrodite character. But it is needed further experimental results to prove it. Tomato yellow leaf curl disease is a serious constraint to the production2012 植物種苗團隊-作物種苗微體繁殖技術之開發與改進 The study of healthy seedling production by tissue culture 101農科-9.1.1-種-X2 101AS -9.1.1-SS-X2 繁殖技術課 Propagation Technology Section 陳哲仁 Jen-Ren Chen 本試驗建立春石斛9117品種組織培養繁殖技術及植株苗期肥培管理條件。使用添加細胞分裂素BA或TDZ之培養基，可顯著的促進芽體增殖數，以B4、T2培養2個月每培殖體平均可形成3.12芽、3.23芽，顯著高於對照組之1.6芽。增殖之芽體經移至添加NAA和香蕉粉之培養基培養，具有誘導根系形成之效果，以N1B3培養基培養2個月後根數達5.6根，根長達4.1 cm且發根率達100.0%為最佳。將組培苗出瓶種植10個月之植株，進行不同氮、磷、鉀比例之營養液處理，試驗對其苗期生長、開花表現之影響，結果顯示，苗期植株外表性狀之生長以低量的磷、鉀肥處理(N1-P1-K1、N1-P2-K1)較佳，然後續之開花表現則以苗期施用中量之磷、鉀肥(N1-P2-K2)之表現最佳。訪視春石斛蘭業者，確認主要病害為炭疽病及葉斑病。採樣病株，進行組織分離，得到4株炭疽病菌，並回接5品種石斛蘭葉片，確認病原性。本研究為探討3品種鳳梨不定芽形成，以0.5 mg/L TDZ 處理之側芽培植體癒傷組織誘導率最高，為80%，培養3 週後已有不定芽形成。而不定芽形成率則以添加3.0mg/L BA最高，為98%。在不定芽誘導發根試驗結果顯示以2.0 mg/L NAA或IAA之發根率95% 為最佳，平均發根數有2.1根形成。種苗金皇一號石斛冷水萃取物可以活化小鼠巨噬細胞(RAW264.7 cells)促進NO的釋放，而且也可以明顯促進TNF-α 和IL-6的釋放。 An efficient in vitro propagation protocol for nobile-type dendrobium was established. In this study a shoot proliferation medium supplemented with different concentration of cytokinins (BA or TDZ) was evaluated its effect on shoot proliferation. The results indicated that using shoot proliferation medium B4 or T2 were optimal for shoot proliferation with shoot proliferation number per explant of 3.12 3.23. The rooting rate of plantlets were 100% after two months rooting in the rooting medium –N1B3 the average root number and root length of plantlets were 5.6 and 4.1 cm respectively. In addition the purpose of this study was to evaluate the effect of fertilizers with different rate of nitrogen (N) phosphorus (P) and potassium (K) on the growth and flowering of the nobile-type dendrobium. The results show that treatments of N1-P1-K1 and N1-P2-K1 were optimal for growth but the N1-P2-K2 treatment was optimal for flowering.We have visited 2 Dendrobium entertainments confirming the maindiseases are anthracnose and Pseudocercospra leaf spots. By sampling diseased plants and tissue separating 4 strains of Colletotrichum spp.were obtained. Leaves of 5 Dendrobium varieties were inoculated with these strains for making sure their pathogenicity.The objectives of the present study were to develop culture method for shoot formation in 3cultivars of pineapple. Treatment of 0.5 mg/L TDZ was found best in callus induction from lateral bud explants2012 植物種苗團隊-仙履蘭產業技術開發及應用 Paphiopedilum industry technologydevelopment and application 101農科-9.1.1-種-X3 101AS -9.1.1-SS-X3 生物技術課 Biotechnology Section 廖玉珠 Yu-Ju Liao 不公開 Not public2010 作物種原保存利用與品種資訊體系之建立 The collection and regeneration of germplasm in crops 99農科-8.1.1-種-X1 99AS-8.1.1-SS-X1 品種改良保護課 Plant Breeding Section 林正雄 Cheng-Hsiung Lin 不公開 Not public2010 台灣香藥草植物資源開發利用 Development and utilization of indigenous aromatic herbs resources in Taiwan 99農科-8.4.1-種-X1 99AS-8.4.1-SS-X1 繁殖技術課 Propagation Technology Section 羅英妃 Ying-Fei Lo 不公開 Not public2014 提升種子檢查量能模式之建立 Establishment of the testing system to promote seed test capacity 103農科-6.2.3-種-X5 103AS -6.2.3-SS-X5 種苗經營課 Seed & Seedling Management Section 黃崧銘 Sung-Ming Huang 種子品質檢查除仰賴檢查人員專業知識與豐富經驗外，完善的儀器設備亦有助於確保種子品質並提升檢查效率，進而降低農作物生產風險。本計畫採購立體顯微鏡、分樣器、電子天平、鏡檢台、防潮箱、實驗桌，建立細小種子潔淨度檢測流程，並經兩位新進同仁進行盲樣測試，結果顯示，對於缺乏分析經驗的同仁，可遵循新建立之檢測流程快速上手，大幅縮短水稻室內檢查時間。 The quality of seed testing is based on not only well-experienced professional inspectors to ensure the seed quality but also well-found equipment which improve efficiency of testing to reduce the risk of crop production. In order to establish the seed purity testing procedure and shorten the examining time the equipmen of stereoscopic microscopes sub sampler electronic scales microscopic units cabinets and benches were purchased in this project. A preliminary test procedure has been built and prove by two colleagues using blind sample test. The result indicated that follow the improved process of seed testing can greatly reduced the test time even for Inexperienced colleagues2014 水稻種子影像辨識輔助系統之研究與開發 A study and development of inspection system for rice seeds with image processing 103農科-7.1.1-種-X1 103AS -7.1.1-SS-X1 種苗經營課 Seed & Seedling Management Section 許鐈云 Ciao-Yun Syu 不公開 Not public2014 建構亞太植物種苗產業服務資訊平台 Construction for Asia Pacific Seedlings Industry Services Platform 103農科-7.1.1-種-X2 103AS -7.1.1-SS-X2 技術服務室 Technical Service Section 蔡瑜卿 Yu-Ching Tsai 亞太植物種苗產業服務資訊平台目前共累積512663筆資料，瀏覽人次866103人次。本年度配合本場辦理教育訓練、展示會、觀摩會等各項活動積極宣傳行銷本平台，增加本平台曝光率；使用者意見回饋分析顯示功能使用上多集中在最新消息與最新科技成果兩大單元。透過技術盤點與文獻分析了解目前國內外相關種苗知識缺口與技術重點，其分析結果除可供國內相關技術研發考量外，現以「產業分析專欄」與「重要專利情報」兩種形式，供亞太植物種苗產業服務資訊平台會員市場面之參考。蝴蝶蘭品種性狀影像辨識系統為協助提升植物品種智慧財產權性狀檢定效能之輔助工具，目前已輸入約400筆以上品種影像資料，隨著資料量越多，系統之辨識效能也會隨之提升，對業務效能之協助提升也更具助益。 "Pacific plant seed industry information platform for a cumulative total of 512663 documents visits 204238 people in 2014. Function uses the latest news and more concentrated in two industrial services unitwhich shows the current platform is still aware of the low should take a more active growth platform for exposure. Inventory through technical understanding and knowledge gaps in the current focus on domestic and foreign technology in addition to its analysis of the results of research and development of related technologies available for domestic considerations the present in two forms \""industry analysis column\""and \""important patent information\"" for the Asia-Pacific plant seed industry market information platform serving member of the reference plane. Future technologies are expected to be applied to the theme of the series of textbooks2014 番茄抗萎凋病與菸草嵌紋病毒分子標誌建立與應用 The establishment and application of specific molecular markers for TMV and fusarium wilt resistance in tomato 103農科-9.1.1-種-X1 103AS -9.1.1-SS-X1 生物技術課 Biotechnology Section 孫永偉 Yung-Wei Sun 1. 番茄抗萎凋病基因型之分子鑑定：番茄萎凋病為重要真菌性病害，本試驗建立抗萎凋病基因I-1及I-3之分子標誌。利用I1-At2引子組可同時擴增番茄抗病(I-1)與感病(i-1)基因150 bp之DNA條帶，將抗感病品種進行PCR產物解序後，於序列位置80出現差異點，該位置感病核苷酸序列為C，抗病核苷酸序列為G，應可作為判斷番茄抗感萎凋病基因I-1之SNP標誌。利用I3-P7-CAPS引子組配合限制酶NsiI酶切反應，可擴增抗病基因(I-3) 380 bp之DNA條帶，感病基因(i-3)無DNA條帶出現。此分子標誌檢測結果與亞洲蔬菜中心及國內育種者或種子公司已知抗感性品種吻合。2. 番茄抗菸草嵌紋病毒分子標誌之建立：菸草嵌紋病毒為菸草鑲嵌病毒屬(Tobamovirus)，為番茄三大病毒之一。本研究利用亞洲蔬菜中心提供番茄抗感病品種進行研究，本試驗針對抗病基因(Tm-1及Tm-22)與TMV病毒各篩選專一性分子標誌(Tm1-CAPS、Tm22-SCAR與TMV-012)。利用Tm1-CAPS分子標誌配合HaeIII限制酶反應後，可擴增抗病基因550 bp之DNA條帶、擴增感病基因350 bp之DNA條帶。利用Tm22-SCAR分子標誌，可擴增Tm-2抗病基因255 bp之DNA條帶、擴增Tm-22抗病基因214 bp之DNA條帶，感病品種無DNA條帶出現。TMV-012分子標誌可擴增嵌紋病毒株(TMV-0、TMV-1、TMV-2) 2.2 kb之DNA條帶。上述分子標誌可協助育種者早期篩選抗病植株、確認抗感病基因型及病毒感染情形，提高育種效率。 The projects of this program have ttwo components including:1.Molecular identification of tomato genotype resistant to Fusarium oxysporum: tomato fusarium wilt is one of the important fungal disease.The molecular markers linked to fusarium wilt resistant genes I-1 and I-3 have been developed. Specific primer pair I1-A2 was developed to amplify DNA fragment from vary allelic at I-1 locus. A 150 bp DNA fragment was amplified from both resistant and susceptible tomato accessions that corresponding to the presence of I-1 and i-1 alleles.After sequencing of PCR products G is determined at the nucleotide sequence position no. 80 in resistance tomato accession where as C is determined in the susceptible tomato accession. The different nucleotide sequence between both of resistance and susceptible tomato accession provide the possibility for development of SNP marker. In addition the I3-P7-CAPS marker was also developed PCR following restriction enzyme (NsiI) digestion. The 380 bp DNA band was obtained from the tomato accession with resistance genes I-3 however the susceptible tomato accession with gene i-3 was none band. The results are also confirming the previously study of AVRDC- The World Vegetable Center and other seed companies.2.Development of molecular marker linked to tomato mosaic virus (ToMV)resistance: ToMV a member of Tobamovirus is one of the important tomato viruses and ToMV-0 ToMV-1 ToMV-2 are three major ToMV strains. The Tm-12014 作物種苗微體繁殖技術之開發與改進-綠竹、鳳梨、春石斛及中草藥繁殖開發利用 The study of healthy seedling production by tissue culture 103農科-9.1.1-種-X2 103AS -9.1.1-SS-X2 繁殖技術課 Propagation Technology Section 張珈錡 Jia-Ci Chang 1.綠竹組織培養芽體形成於MS+0.1 mg/l NAA+3 mg/l of BAP，發根的誘導採用以2芽為一芽團培養於MS+0.01 BAP mg/L + 2.0 mg/L NAA，發根芽體於溫室中進行2週的馴化再移植至塑膠容器中培養，最後移至田間栽培，成活率100%。2.鳳梨組織培養可使用冠芽分生組織大量繁殖，試驗結果利用BAP及NAA不同組合在誘導芽體的增殖，最優化的培養基為MS培養基添加2.0 mg/L BAP。3.板藍根組織培養，以側芽為培植體進行增殖培養，其中培養三週後以1/2MS＋2mg/L BAP＋0.1 mg/L IAA，叢生芽體形成率高達95.2%，在1/2MS＋4 mg/L BAP＋0.1 mg/L IAA最高芽體形成率最高(98.5%)。4.地黃的根改改善便秘之動物模式評估，本研究以每日餵食地黃的根可否改善大鼠的便秘。結果顯示，餵食Loperamide 1% 及5% TP的處理對緩解大鼠便秘對糞便的乾重及其中水分含量並無影響。5.春石斛組培苗出瓶種植後之植株後續生長表現仍受到增殖階段培養基成分之影響，以低濃度MS基礎鹽類培養基(1/4MS)、高濃度馬鈴薯粉(2-4g/L)和蔗糖(20-30g/L)所增殖培養之組培苗，其植株後續生長表現較佳，顯示組培瓶苗之品質於栽培初期顯著的影響植株之生長表現。6.春石斛組培苗於不同月份種植，初步觀察對植株之成活率無顯著之影響。7.金童石斛萃取物緩解第二型糖尿病試驗中，因誘導小鼠產生病徵不穩定，故無法作為給藥後觀察 。然另經動物細胞試驗，結果顯示金童石斛萃取物具有調節免疫系統、抗發炎的功能。 1.In Bamboo (Bambusa oldhami Munro.) In vitro axillary shoot formation was successfully made in MS basal medium supplemented with 0.1 mg/l NAA and 1.5 mg/l of BAP. Clamps of at least 2 shoots were used for root induction in MS medium with 0.01BAP mg/L and 2.0 mg/L NAA. Rooted plantlets were successfully acclimatized in green house for 2 weeks and then were transferred to the plastic container. 100% survival rate was recorded after field transfer.2.In vitro mass-propagation of Ananas comosus L. (pineapple) in tissue culture system was carried out using crown tip meristem. The result shows that the crown tip managed to induce shoots in different concentrations of BAP.3.Optimum shoot proiferation was obtained onto MS medium supplemented with 2.0 mg/L BAP and MS medium containing 1.0 mg/L BAP/0.5 mg/L NAA.In vitro Isatis tinctoria L. Treatment of 1/2MS＋2 mg/L BAP＋0.1 mg/L IAA was found best in multiple shoots induction from lateral bud explants induction percentage reached 95.2% shoots were formed after culture for 3 weeks. The highest percent shoot formation which is 98.5% was found in the treatment of 1/2MS＋4 mg/L BAP＋0.1 mg/L IAA.4.Roots of Rehmannia glutinosa as a Diet for Improvement of Constipation in Rats. This study was conducted to determine effects of Rehmannia glutinosa roots (TP) as a diet for improvement of constipation in rats.Results showed that the rats treated with Loperamide and fed with 1% or 5% TP had no significant in fecal weight dry stool weight and water content (%) of feces (P ＜ 0.05) compared to the negative control.5.The growth performance of Dendrobium after planting in greenhouse still affected by shoot proliferation medium components in the tissue culture stage. The the plantlets cultured from shoot proliferation medium supplement with a low concentration MS basic medium (1 / 4MS) high concentrations of potato (2-4g / L) and sucrose (20-30g / L) its subsequent growth performed better results showed the quality of tissue culture plants will significant effects on the growth performance in the early cultivation.6.There is no significant effect on the survival rate of Dendrobium plantlets that planting in different months.7.In this study2014 仙履蘭產業技術開發及應用 Development and application of Paphiopedilum industry technology 103農科-9.1.1-種-X3 103AS -9.1.1-SS-X3 繁殖技術課 Propagation Technology Section 廖玉珠 Yu-Ju Liao 1.建立仙履蘭maudiae type 品系之微體繁殖體系，以仙履蘭開花株之分蘗芽或花苞等部位誘導出之芽體探討繼代增殖及發根之最佳培養條件 。以綠maudiae typy 'The Queen'之分生苗增殖階段每代培養時間對增殖倍率之影響—培養時間長增殖倍數有較高之趨勢，以每次繼代培養時間為2.5、3、4、4.5個月，結果顯示：以培養4個月之增殖倍數最高，分別為1.93、1.51、2.83、2.4。另於發根階段培養於含鉀濃度20mM之培養基中，不論在株高、葉幅、根數、根長表現最佳。若鉀濃度提高至40mM根長明顯下降顯示根的生長受到抑制。2.以Comple type 4266及Maudiae type 4321為材料於不同溫控溫室栽培1年後，以栽培於一般溫室的對照組生長稍佳。在高溫30/28的處理下生長狀況最差，而Maudiae type 4321較能耐受高溫的處理，Complex type則相對較適應大溫差環境。在不同溫控溫室及海拔栽培下，Complex type 4518開花率可達76.7%且開花較整齊，高溫差或較涼溫的環境下，有加速開花之作用，但相對栽培於梅峰、春陽其敗育率也較高，而Maudiae type-4321則開花較不具一致性，二者之開花與溫度處理似乎無直接關聯性。分析其糖類成分與開花的關係，可觀察在9月花苞形成的月分，會消耗大量性可溶性糖類，但是C/N易受環境影響，因此目前並不適用為仙履蘭花朵誘導指標。3.以葉面施肥方式每週施用不同濃度之N(0、50、100、200、400 mg.L-1)、P(0、25、50、75、100 mg.L-1)、K(0、50、100、200、400 mg.L-1)營養液，評估對仙履蘭maudiae type 9905品系植株初期生長(1-6個月)之影響。試驗顯示，仙履蘭初期生長仍以低肥料濃度為佳(50 mg.L-1N、25 mg.L-1P和0 mg.L-1K)，但較高之肥料濃度(400mg.L-1N、100 mg.L-1K以下)對植株之地上部和地下部之生長亦無顯著的抑制作用。因此，採用葉面噴施低濃度營養液之肥料施用方法，應可作為仙履蘭種苗栽培初期肥培管理之參考。 According former researches It is difficult in vitro propagation between Interspecies of slipper orchids. To establish in vitro propagation for subgenus of Paphiopedilum will be done.To improvement Paphiopedilum in vitro propagation system.Complex type 4266 and Maudiae type 4321 seedlings cultured in different day-night temperature after 1 year the result indicate that the growth coefficient of control group is better and cultured in 30/28 day-night temperature made both of two type growth worse.In the resultMaudiae type4321 also showed a better tolerence to high temperaturewhile Complex 4266 could adapted large temperature variation than Maudiae. In addition cultured under different day/nigh temperature and high altitude the flowering rate of Complex type 4266 could up to 76.7%and with a better uniformity. The flower of Complex type 4266 also developed more quickly under larger temperature variation or cooler temperature but aborted more easily (in Mei-feng and ChunYang).Meanwhile Maudiae type 4321 has a ununiformed flowering habit. Due to the result seems both of their flowering habit do not have directly relationship with temperature. As to the testing of total soluble sugar2014 建立硬質玉米種子籌供體系 Establishment of hybrid corn production and supplying system 103農科-9.2.2-種-X1 103AS -9.2.2-SS-X1 農場 Research Farm 陳學文 Hsueh-Wen Chen 計畫於台中市新社地區，以台農1號及台南24號玉米兩品種，進行秋季栽培，並以不同去雄方式及保留穗上節位數處理，調查對環境適應性、生育特性及產量之影響，以作未來量產模式之參考。尋找參試品種新採種栽培及調整採種適期以適應氣候變遷，建立量產模式，穩定生產種子，以配合政府政策，生產所需種子。搜集國際商用飼料玉米品種，評估各品種於台灣之適應性，建立適宜台灣種植之國際飼料玉米品種資料庫，期能提供農民多樣選擇並穩定飼料玉米種子供應。調查及紀錄調製過程中，外界氣候條件對穗倉中倉溫及相對濕度之影響，以及利用不同乾燥處理方式探討對玉米種子水分變化、種子發芽率、發芽勢等之影響，期能達最佳能源利用效率及品質兼顧。 We conducted different planting dates for Tainan no.24 parents in Taichung area for autumn corp. To discuss the influence for number of leaves on the ear of corn after detasseling on seed production of c o r n . I n v e s t i g a t i o n o f a d a p t a b i l i t y t o e n v i r o n m e n t a n d g r o w t h characteristics and yield as reference for future production models.This year we introduced three new varieties including S11 S12 and 102.According to the first-year observation the heading stage of S11 is similar to Tainan No.24 and TNG1 and has the best yield performance in the spring.S11 is evaluated to be a early maturing commercial variety but further observation are needed.This research investigated the effects of climate conditions on the temperature and the relative humidity in storehouses of corncobs and used different drying methods to discuss the influence on the variation of corn seed moisture content germination rate and viability. The object of the research was to achieve both the best energy-using efficiency and the seed quality in storage. The data from the experiment showed that temperature in storehouses of corncobs was mainly affected by the moisture content of corncobs at earlier stage of drying process but varied with the atmosphere temperature at middle and later stage. On the other hand the effect of environments on the temperature in storehouses of corn seeds was unapparent because the drying time in storehouses of seeds was short2014 優質番木瓜品種選育 Good quality varieties selection on papaya 103農科-9.2.3-種-X1 103AS -9.2.3-SS-X1 屏東種苗研究中心 Pingtung Seed & Seeding Research Center 邱展台 Chan-Tai Chiu 本年度進行優良耐儲運品系f7選拔，並進行雜交，供選拔優良組合進行後續試驗。果皮光滑品系選育，係以Golden為親本之雜交第四代，進行選拔。其雜交後代的果皮生理性斑點仍相當多，經第3代分離，共選出14株果皮較光滑特性的單株。其著果數65-142粒果重314-890公克，總可溶性固形物11.5-15 %Brix，果實品質優良。耐儲運品系選育，以引進自東南亞的品系與本場育成之品系為雜交，經7代選拔之選拔純化，共選出12個品系，12個品系均為紅色果肉，果重327-1226公克，果實數目58-123果，多為西洋梨形之中小型果，果實總可溶性物11.5-15.4 % Brix ，採收5天後之果肉硬度以103- P16及103-O38之硬度最高。觀察14個耐儲運之新組合雜交一代其果實品質及儲運性均優於台農二號，但產量則低於台農二號，各項性狀仍需進一步觀察。 The resistant character of storage and transportation were selected.Hybrid combinations for subsequent comparison test had been done. Peel smooth less flecks of strain providing isolation and selection for next year. Peel smooth breeding program utilize Golden variety for the parents after separation of the fourth generation were selected. The flecks of third generation were still severe. Fourteen lines with Peel smooth characteristic were selected. The fruits number are 6 5 to 142 per plant and fruit weight are 314 to 890 grams total soluble solid are 11.5~15 Brix good fruit quality. Breeding program of tolerant to storage and transportation was conducted utilizing introduced strains from Southeast Asia. Through 7 generations segregation and purification12 lines were obtained. All 12 strains are red pulp medium sized fruit of the pear-shaped high sweetness. All twelve lines’ fruit have higher firmness and the flesh softens speed slower than the Tainoun No2.Observes 14 to tolerant the storage and transport the new combination.The fruit quality and the storage and transport character surpasses Tainoun no.22014 仙履蘭及熱帶球根花卉品種改良與種苗生產技術開發 Improvement on slipper orchid and tropic bulb flowers breeding and establishment the seedling production 103農科-9.2.5-種-X1 103AS -9.2.5-SS-X1 品種改良保護課 Plant Breeding Section 劉明宗 Ming-Chung Liu 依照仙履蘭育種四大目標進行雜交授粉工作，103年篩選優良雜交後裔PA95110及PA95008組合，PA95110雜交後裔組合為綠葉中型植株，於唇瓣會有鮮豔的紫紅色特徵，翼瓣邊緣波浪，適合單花或組合盆觀賞；PA95008雜交後裔組合為綠葉單花、綠紅花及上萼瓣渾圓特性，可作為組合盆觀賞。孤挺花已選拔出具潛力單株3株，香氣育種方面，以氣相層析-質譜儀(GCMS QP-2010 Shimadza Tokyo Japan)分析香氣成分，從親本圃中篩選出具香氣親本分別是 ‘Estella’的主要香氣成分有羅勒烯、芳樟醇、金合歡烯及反式-橙花叔醇等； ‘Trendsetter’ 主要香氣成分為β-芳樟醇、桉油醇、羅勒烯、α-松油醇及金合歡烯等；‘Blossom Peacock’之主要香氣成分是桉油醇、β-芳樟醇、羅勒烯、環己烯甲醇及金合歡烯等，可作為育種親本。目前已選拔出‘種苗一號粉珍珠’新品種。其花朵會散發出輕微香氣。其香氣主要成分是桉油醇、β-芳樟醇、羅勒烯、Linalool oxide、環己烯甲醇 及金合歡烯等。在彩色海芋方面，103年雜交組合共30組合，以金黃色系與紫黑色系為主；94及95年雜交後裔中已篩選具潛力彩色海芋單株3株，為金黃色、紅色及紫黑色。花朵數2-3朵，其中紫色單株花莖較短，可為盆花，其餘為切花用途。在宮燈百合利用不同切球方式對於宮燈百合種球養成之影響，在地上部生育部分，植株最大葉長、花朵數、結束開花節間位數之性狀上均為顯著性差異，以不切球方式之生育性狀綜合表現為最好，但萌芽總數則以切球處理表現最佳。金花石算部分則是利用GA3浸漬種球可促進種球萌芽天數縮短，大球比小球的50%萌芽日數及萌芽終止日數較短。 According to four goals in slipper orchids breeding we have selected superior progenies ‘PA95110’ and ’PA95008’ with multi-flowers good flower shape and color characteristics. Bulbous of amaryllis breeding we have selected 3 potential hybrid progenies of double-flower type and selected 3 cultivars with fragrance for fragrance breeding. In fragrance amaryllis breeding we have bred new variety‘T.S.S. No.1-Pink Pearl’which flowers emit a light fragrance. The aroma composition is Cineolebeta-Linalool 1.3.6-Octatriene Linalool oxide 3-Cyclohexene-1-methanol and alpha-Farnesene. Calla lilies breeding2014 春石斛開花株生理指標及花期調節管理體系之建立 Set up nobile-type dendrobium mature index and flowering regulation systerm 103農科-9.2.5-種-X2 103AS -9.2.5-SS-X2 品種改良保護課 Plant Breeding Section 郭孄婷 Lan-Ting Kuo 不公開 Not public2014 植物品種檢定流程分析、人員訓練及分子標誌開發 Analyzing the examining process flowtraining faculty members and developing molecular marker of plant variety right 103農科-9.2.6-種-X1 103AS -9.2.6-SS-X1 生物技術課 Biotechnology Section 張惠如 Hui-Ju Chang 不公開 Not public2014 植物品種開發及種苗驗證之應用研究-植物種苗認驗證體系建立 Construct the accreditation system for plant seedlings 103農科-9.2.6-種-X2 103AS -9.2.6-SS-X2 生物技術課 Biotechnology Section 周佳霖 Chia-Lin Chou 一、健康種苗之母本檢測與母本園建構及維護：本年度完成馬鈴薯12品種(系)及鳳梨3品種病毒檢測，所測樣品皆無血清反應。並完成母本園設施改善與建置，可供健康種苗母本保存及檢測研發利用。另外，利用實驗室可取得之8種不同材質吸附膜，進行馬鈴薯病毒 Y 之汁液印漬捕捉反轉錄聚合酶鏈鎖反應(Print Capture RT-PCR PC-RT-PCR)檢測技術測試，以建立可縮短抽取核酸的人力物力之檢測技術，試驗結果以CALBIOCHEM公司產品Miracloth為最佳。二、ISTA豆類種子健康檢查驗證體系之建立：為建立種子檢查室之豌豆葉斑病菌與菜豆炭疽病菌檢測作業流程，本年度依國際種子檢查協會公告之檢測方式建立檢測流程，並實際測試檢測流程的穩定性。三、種子(苗)品質純度分子檢測技術研發及標準化：本年度以葫蘆科西瓜為基礎，開發20組SSR與16組SNP品種檢定分子標誌，經篩選後計有14組SSR與13組SNP標誌可檢定計22個不同的西瓜雜交組合，且計有至少93個多型性表現。 In this study virus diseases in 12 varieties of potato and 3 varieties of pineapple were tested and all samples would have produced negative results. In addition facilities of mother-stock house had been improvedthat would benefit healthy seedling conservation and development. The 8 different kinds absorbent membranes were test to instead the RNA extraction protocol before RT-PCR for PVY detection. The most efficient material was Miracloth which was production by CALBIOCHEM. For establishment the health check of the seed beans verification system by ISTA the objective of this program is to establish the seed testing procedures on Ascochyta pinodes and Colletotrichum lindemuthianum. The procedure in coincidence with ISTA rules has been established and confirmed the stability. To increase industrial competitiveness 20 SSR and 16 SNP markers had been developed in this project for testing hybridization seeds based on cucurbitaceae. Results showed that 14 SSR and 13 SNP markers distinguish the hybridization purity in 22 watermelon hybrids and represent at least 93 polymorfics.2014 種子品質及處理技術研發 Developement for the seed processing techniques to promoting seed quality 103農科-9.2.6-種-X3 103AS -9.2.6-SS-X3 種苗經營課 Seed & Seedling Management Section 黃玉梅 Yu-Mei Huang 不公開 Not public2014 植物新品種檢定技術之開發與執行 Plant Seed Group-The Technical Development and DUS Test Execution of Plant Variety Protection System 103農科-9.2.6-種-X4 103AS -9.2.6-SS-X4 品種改良保護課 Plant Breeding Section 安志豪 Chih-Hao An 本(103)年度已完成草擬狐狸尾蘭及合果芋並修訂文心蘭與彩葉芋品種性狀表與試驗檢定方法，透過增修訂品種性狀表及試驗檢定方法，擴增國內植物品種權受保護之植物種類。關於蝴蝶蘭及玫瑰等作物資料庫建立方面，已收集15個蝴蝶蘭、5個玫瑰商業品種並完成品種性狀調查及資料庫建置，透過資料庫建置，以利未來對照品種之資料比對搜尋。本年度受理新品種性狀檢定工作計蝴蝶蘭與朵麗蝶蘭85件、文心蘭5件、玫瑰20件及桂花5件；正進行性狀檢定中之案件為蝴蝶蘭與朵麗蝶蘭30件、文心蘭6件及玫瑰8件；檢定完成資料整理中為蝴蝶蘭與朵麗蝶蘭35件、文心蘭4件及蕙蘭1件；檢定完成且審查結束為蝴蝶蘭與朵麗蝶蘭80件、文心蘭8件及玫瑰1件，透過植物品種權制度，確保植物育種者權利及品種保護之效力，同時也提升農業產值之提升。 The purpose of this project is to establish the test guidelines and execute DUS tests. We modified the test guideline and table of characteristics of rhynchostylis araceae Caladium × hortulanum and oncidium. We also collected 15 commercial varieties of phalaenopsis and doritaenopsis and 5 commercial varieties of rose and investigated their characteristics for establishing variety database. We accepted 85 cases of phalaenopsis and doritaenopsis 5 cases of oncidium 20 cases of rose and 5 cases of osmanthus to execute DUS tests for protecting the breeder’s rights this year. 30 cases of phalaenopsis and doritaenopsis6 cases of oncidium 8 cases of rose have been executing DUS tests. 80 cases of phalaenopsis and doritaenopsis 8 cases of oncidium and 2 cases of roses have been granted to be new varieties.2014 馬鈴薯種薯與藥用植物種苗量產體系建立 Establishment of mass production system of seed potato and medicinal plant seedling 103農科-9.2.6-種-X5 103AS -9.2.6-SS-X5 繁殖技術課 Propagation Technology Section 王至正 Jhih-Jheng Wang 不公開 Not public2014 作物遺傳種原收集、保存與利用 Preservation collection and application of germplasm 103農科-9.2.6-種-X6 103AS -9.2.6-SS-X6 品種改良保護課 Plant Breeding Section 薛佑光 Yu-Kuang Hsueh 蔬菜種原的收集與繁殖為品種改良之重要來源，為確保種原庫種子活力和繁殖能力，因此有計畫地分年進行繁殖更新是有其必要性，今年度完成102冬-103春年期芥藍26品系、結球白菜40品種、青花菜6品種，夏作10個西瓜種原、12個萵苣品種，秋作2個豇豆地方品種之蔬菜種原栽培管理、性狀調查、授粉採種、種子調製、秤重、包裝及貯藏於種原庫等。並進行103年冬-104年春年期59種蔬菜種原育苗、定植栽培。種原利用先進行西瓜花粉保存技術之研發，以西瓜品系‘402’、‘405’及‘408’為參試材料，採收開花當日之新鮮花藥，經氯化鎂乾燥24小時後，花粉發芽率低於未乾燥及乾燥5小時之處理，但經液態氮保存1個月後，花粉發芽率則相對高於該兩處理。原生綠化苗木103年度收集桃實百日青、蚊母樹、疏脈赤楠、十大功勞、杜虹花、蘭嶼裸實、細葉杜鵑、白木蘇花、大果厚殼桂、細葉蚊母樹、蘭嶼樹杞、風箱樹、莢蒾、台東石楠、壽娘子、恆春楨楠、枯里珍、台灣櫸、水社柳及台灣野牡丹藤等20種原生綠化苗木分別為2種金縷梅科、2種馬鞭草科、2種樟科，紫金牛科、羅漢松科、杜鵑花科、蘇木科、姚金釀科、衛矛科、小蘗科、茜草科、忍冬科、薔薇科、大戟科、榆科、楊柳科、野牡丹科各1種。蜜雪梨品種(農試種苗二號)係農業試驗所早年以高海拔新世紀梨與平地橫山梨雜交之後裔，經本場多年選拔所育成品質優良之梨品種。於民國八十三年七月命名通過，本品種適合海拔400至800公尺左右地區栽培，屬低海拔地區之低溫需求梨，生長勢強、低溫需求少、不需再高接、產量高、果實碩大、果肉雪白、不易褐化、甜脆多汁及清爽可口，甚受消費者喜愛。103年度特定選擇生長較優勢的100植株，進行有系列栽培管理。於102年底進行樹幹修剪整枝建立優良樹型。於修剪後施有機質肥料每棵20公斤。103年起陸續進行病蟲害防治、疏果及果實套袋作業。7月份蜜雪梨果品採收量為2388公斤，果品糖度平均為11.12Brix。其中選擇3株樹勢較佳樹體進行嫁接，與未嫁接蜜雪梨果品比較，嫁接處理果品重526.3g﹔未嫁接處理果品重540.2g。嫁接處理果品糖度為10.8 Brix﹔未嫁接處理果品糖度為11.2Brix。木蘭科綠美化苗木全年收集11種種原，分別為烏心石、蘭嶼烏心石、白花辛夷、紅花辛夷、粉花辛夷、紫花辛夷、夜合、白玉蘭、黃玉蘭、含笑及紫花含笑。植株生育健壯後，再調查生長狀況及性狀等以待種原保存用。其中以黃玉蘭為試驗材料，利用三種栽培介質作為處理，調查每階段黃玉蘭種苗之生育情形，總體表現看來以泥炭土為育苗介質處理比泥炭土、珍珠石、蛭石介質混合比例1:1:1及田土生育效果佳，將繼續觀察各處理苗株生育表現，期能選出最適景觀綠化容器苗管理模式。台灣本土多樣性資源豊富，利用既有的農業科技基礎，保存植物種原，經濟栽培台灣野生植物及開發利用植物種子種苗有利於生態保育。香藥草植物資源之保存及維護約600種，並進行薑科植物萃取物保濕性測試及利用18種香藥草植物萃取液進行防蚊測試，其中以5種精油配製的複方防蚊膏比市售的產品效果佳。茄科及瓜類蔬菜種原計畫分別收集亞洲地區胡瓜及茄子種原，進行栽培及種原更新，同時調查植株生育特性，初步篩選出20個高雌性胡瓜品系及20個質優耐病的茄子品系，其中20個胡瓜品系皆具有單為結果性特徵，茄子在耐青枯病觀察方面，有5個品系耐病性表現較佳，可作為育種之利用及試驗材料之選擇。 R e n e w e d v e g e t a b l e g e r m p l a s m f o r 9 4 v a r i e t i e s o f c u l t i v a t e d investigation harvesting packaging and stored seed. Including 12 new lettuce varieties We had renewed 26 species of kale 40 species of Chinese cabbage 6 species of broccoli and 10 species of watermelon in this year. In the meanwhile we had completed the characteristic investigation of all. Anthers of water melon lines ‘402’、‘405’及‘408’ were used as materials in the pollen preservation experiment.Generally speaking pollen germination percentage of anthers dehydrated for 24 hours by magnesium chloride was lower than those of non-and 5 hour-desiccation treatments. However2014 蔬菜有機種衣劑及種子有機處理基準之研究 Establishment of vegetables organic coating material and organic processing benchmark in seedling 103農科-9.2.7-種-X1 103AS -9.2.7-SS-X1 種苗經營課 Seed & Seedling Management Section 蘇士閔 Shih-Min Su 在有機種衣劑部分，本試驗以大豆、毛豆、紅豆、四季豆、花生及玉米為材料進行有機配方的種子披衣試驗，使用底衣粉：高嶺土及滑石1：1及底衣液：10% 阿拉伯膠、2.5%膜衣劑、1%三仙膠皆不影響上述種子發芽率。於大豆及玉米披衣種子中添加500倍辣蒜精、100倍木醋液、500倍苦棟油亦皆不影響種子發芽。大豆披衣種子添加一層100%苦茶粕、菸草粉、辣椒粉、香蒜粉再披衣一層底衣粉及外層加入100%硫磺粉、竹碳粉至不見原披衣層；玉米披衣種子添加一層100% 硫磺粉、竹碳粉、辣椒粉、香蒜粉75%苦茶粕、50%菸草粉後再披衣一層底衣粉之處理與對照組相較下較不影響發芽表現。將添加辣蒜精、木醋液、苦棟油、硫磺粉、竹碳粉、辣椒粉、香蒜粉、金色及桃紅色色粉的玉米披衣種子隨機播種於花蓮農地，第一週發芽率在90-97%之間，第二週後缺株率在1.5 - 3.3株間，各處理無明顯差異。在有益微生物處理部分，叢枝菌根菌及蕈狀芽孢桿菌在不同接菌方式對西瓜種子的發芽率與幼苗的生長均無顯著影響，但對豌豆苗期具有促進生長的效果。 Soybean green soybean red bean string bean peanut and corn were used for this seed coating experiment. Kaolinite and talc mixed 1:1 and combine with 10% Aracica gum 2.5% film-coating agent and 1% Xanthan gum did not influence the germination percentage after coating. The coated seeds did not influence the germination percentage compared with control by following treatment: added chili and garlic extract x500 wood vinegar x100 neem oil x500 of soybean and corn seeds; added one layer of 100% tea seed pomace tobacco powder chili powder garlic powder then added one layer of coating powder on the surface; added 100% sulphur powder and biocarbon powder to the surface of coated soybean seeds; added one layer of 100% sulphur powder2011 番木瓜種苗七號全兩性株調控基因分析與產業應用 Analysis of regulation genes of allhemaphrodite papaya cv. TSS No.7 and its application on industry 100農科-1.1.8-種-X2 100AS -1.1.8-SS-X2 繁殖技術課 Propagation Technology Section 李美娟 Mei-Jiuan Lee 不公開 Not public2011 控制木瓜全兩性株分子機制探討(委辦) Study on molecular mechanisms controlling all hermaphorodite papaya cultivar 100農科-1.1.8-種-X3 100AS-1.1.8-SS-X3 生物技術課(屏東科技大學) Biotechnology Section(National Pingtung University of Science and Technology) 張惠如(陳福旗) Hui-Ju Chang(Fure-Chyi Chen) 不公開 Not public2014 番木瓜有機栽培及有機雜交種子生產研究 Study on organic cultivation and organic papaya seed 103農科-9.2.7-種-X2 103AS -9.2.7-SS-X2 屏東種苗研究中心 Pingtung Seed & Seeding Research Center 邱展台 Chan-Tai Chiu 本試驗擬於春夏季定植木瓜，以植株幼年期度過雨季避開疫病危害，並於冬春季果實炭疽病發生率較低時開始成熟採收。以窄域油及石灰硫礦液防治螨類及秀粉介殼蟲危害，並調查以有機肥料取代化學肥料的效果。試驗結果顯示於植株成活後即開始每週施用窄域油及石灰硫礦液可以抑制螨類及秀粉介殼蟲的發生。木瓜每株分別施用4、8、12公斤有機粒肥等3種施肥量，以施用12公斤之結果數最多，且施肥量與結果數有顯著的線性關係。木瓜果實糖度在有機及慣行栽培間無顯著差異。亞磷酸於雨季無法抑制木瓜成熟果實的病害發生，8月雨季期間，果實的疫病及炭疽病達100%，農藥亦無法抑制。 Papaya were planted on spring and summer respectively young plant passed the rainy season to plant to avoid disease hazards and to begin to mature at lower incidence of anthracnose of fruits in winter and spring. By the narrow range oil and lime sulphur fluid preventing and controlling man kind of and the scale insect harm and investigates the effects of the organic fertilizer substitution chemical fertilizer.Apply employ the narrow range oil and the lime sulphur fluid may suppress mites and the scale insect's occurrence. Papaya plants apply 4 8 12 kilogram organic granulated fertilizer respect apply 12 kilogram result in fruit numbers to be most also the quantity of fertilizer and the fruits number have the remarkable linear relations.The papaya fruit sugar content in organic and tradition of cultivation do not remarkable difference. The phosphorous acid is unable in the rainy season to suppress the mature papaya fruit disease occurrence in August rainy season period fruit epidemic disease and anthrax reach 100% the agricultural chemicals are also unable to suppress2014 提升具競爭力外銷型農糧作物產業價值鏈之研究 Study to make Industrial value chain more competitive for expert in the agricultural seedlings industry 103農科-9.2.9-種-X1 103AS -9.2.9-SS-X1 繁殖技術課 Propagation Technology Section 文紀鑾 Chi-Luan Wen 本計畫擬在國內組織培生產設施，首次導入國際品質管理認證(ISO 9001:2008)系統，強化品質管理能力並作為其他組織培養場推動參考，藉此平台與國際接軌，提升產業之國際競爭力。通過TAF(全國認證基金會)驗證稽核，並取得證書。核發領域為彩色海芋組織培養苗之生產。組培專業證照訓練規劃透過「問卷調查」與「實地或電話訪談」兩方式，針對無菌操作台大於50台以上組培廠商其「基層技術人員」，以及「基層管理人員」，調查其職能需求，並將從中獲得之質性與量性調查結果，進一步進行統計分析，再將初步統整之調查結果，提交於內部意見交流會議討論，以凝聚未來組培專業技術士訓練制度規劃共識。運用種子品質快速檢測技術－Q2種子活力測定儀(Q2)進行番茄種子不同活力種子檢測，透過和標準發芽試驗比較，Q2可有效於14天內判別小果番茄’台南亞蔬6號’及大果番茄’花連亞蔬18號’之不同活力種子品質情況，因此Q2種子活力測定儀可有效判定番茄種子活力品質。以‘臺農2號’商業果實之F2種子為材料，先探討番木瓜種子發酵程度對種子儲藏後發芽率之影響。發酵4日內，無論是新鮮種子或調製後種子，種子活力及活勢並未改變；隨著發酵程度提高，種子活力及活勢會下降，隨著發酵時間越長，而下降程度越大。10oC儲藏3個月種子發芽率也沒有明顯變化。關於調製溫度及含水率對種子儲藏後發芽率之影響，預備試驗中，提高種子調製溫度，不影響含水率30%以下種子發芽率，對於調製溫度 (15、25、35℃) 及含水率 (15%、10%、7.5%) 對番木瓜種子發芽率之影響的試驗，仍在進行中。以防雨之設施栽培健康之種苗，並建構無病毒種苗之驗證體系，建立商業量產之草莓無特定病原健康種苗，避免使用非健康種苗造成的損失，進而建立完整的草莓健康種苗生產系統。於葉長、葉寬部分，組培苗及其走莖繁殖苗之葉片大小平均較農民自行留種苗的小，顯示組培苗的生長式較弱，且具有叢生現象。果實之型態與品質方面，農民自行留種苗之果實於結果初期果實較大，畸形果之比率略隨著組培走莖繁殖苗代數而減少，甜度方面則無明顯差異，故在栽培上，以組培走莖繁殖二代苗以上較有利。以ELISA篩選無特定病原芋頭種苗，並以組織培養建立芋頭健康母瓶。比較4種芋頭增殖培養基，不同IAA濃度對芋頭瓶苗增殖倍率與發根情形等影響並不顯著，然以MS培養基添加IAA 0.01 mg/L之芋頭瓶苗根長最短，對於繼代培養快速增殖操作較為便利。本年度協助業者出具ISTA橙色檢驗證種子計有西瓜、南瓜、胡瓜、辣椒類、番茄、甘藍、菜豆、萵苣、玉米、茄子、花椰菜及青花菜等12種作物共66件，總重16327.5公斤，ISTA藍色檢驗證種子計有有白菜、油菜、莧菜、芹菜、芥菜、玉米及花椰菜等7種，共17件，國內市售種子品質與標示查驗，抽驗322件樣品中達標示之發芽率以上者有305件，未達標示發芽率者計有17件，送檢樣品符合標示發芽率者為95.8%。 "The plan proposed tissue culture production facilities in Taiwanfirst time into international quality management certification (ISO 9001:2008) system strengthening quality management and training as other organizations promoting references use this platform with international standards and enhance the international competitiveness of the industry. Through the TAF (National Foundation for authentication) validation audit and acquired the certificate. Issue areas for the production of micropropagated seedlings of calla lily. Group culture professional certification certificate according to training planning through \\""questionnaire survey\\"" and \\""field or phone interview\\""for Laminar Flow is greater than 50 Taiwan above group culture manufacturers its \\""technicians\\"" and \\""manager\\"".The study is aim to test the different vigor of tomato seeds byQ2 Scanner and try to find rapid seed vigor testing method. Compared to traditional germinations Q2 scanner can identify the different vigor of cherry tomato seed ’Tainan AVRDC No. 6‘ and tomato seed ‘Hualien AVRDC No. 18‘ in 14 days. Consequently2014 原鄉地區原生種作物選育改良、改進作物栽培技術-原鄉馬鈴薯栽培試作及原生豇豆之應用 Study on improvement of native cropvarieties and crop cultivation technology in indigenous area－Test cultivation of potato and application of native asparagus bean in indigenous area 103農科-9.2.10-種-X1 103AS -9.2.10-SS-X1 繁殖技術課 Propagation Technology Section 羅英妃 Ying-Fei Lo 國內8-11月是有機及鮮食馬鈴薯缺貨的季節，原住民部落可利用海拔高度栽培馬鈴薯，以供應夏季的有機鮮食市場。於高海拔設施栽培可以在梅雨季節開始栽種並於8-9月採收，設施栽培薯球產量比露天栽培多一倍，露天栽培則建議於梅雨季節過後，於9-10月採收。中海拔(700m)栽培馬鈴薯比高海拔(2100m)露天栽培產量來得低，於中海拔地區進行不同克尼伯品系營養系的單株產量大約分佈在145-279公克之間，其中以101A、及101G品系之產量表現為最佳，此外，在原鄉部落收集到2個品系，採集後移至本場溫室栽培，在高溫的環境下可以順利產生種薯。原生豇豆以綠皮的品系1產量最高，建議部落種植時將此品系單一種植或混合其他品系種植，可達到較佳的產量。種植原生豇豆品系，期間並無灌溉、施肥及噴化學藥劑，進行粗放栽培即有產量，為省工栽培的有機產品開發，故原生豇豆有機栽培在原鄉部落是具有可行性的。 Market is short of organic and fresh potatoes from August to November in Taiwan. Therefore some aboriginal people can produce potatoes for fresh organic market in summer by taking advantage of higher altitude where they cultivate. People can plant potato in the facility in high land during the rainy season and harvest in August to September which produce twice as yield as those grown in the open field. It is recommended to begin to plant potatoes in the open field after the rainy season and harvest in September to October. Additionally the yield of potato grown in an altitude of 700m in the open field is lower than that in 2100m above the sea level. In the meanwhile the yield per plant of different Kennebec clones grown in the elevation of 700mwas different between 145 and 279 grams which clone 101A and 101G had higher yield. Furthermorewe collected 2 potato clones in the tribal homeland and successfully produced tubers after moving to the greenhouse in our station under high temperature environment. In another experiment native green-skin cowpea strain 1had the highest yield among test strains and we recommended to plant the strain singled or mixed to get the higher yield when grown in the indigenous tribe area. Planting native strains of cowpea with no irrigation fertilization and spraying chemicals could successfully harvest pods2014 農業副產物應用於仙履蘭、春石斛、國蘭、文心蘭等蘭科作物種苗栽培介質之研發 Use of agricultural byproducts as growing substrates for orchids seedling 103農科-9.2.12-種-X1 103AS -9.2.12-SS-X1 繁殖技術課 Propagation Technology Section 張珈錡 Jia-Ci Chang 本試驗利用玉米穗軸、花生殼、香菇廢棄太空包等農業副產物不同比例混合之配方作為替代性栽培介質，評估應用於仙履蘭、春石斛、文心蘭和國蘭4種蘭科作物種苗栽培之可行性。由試驗結果得知，仙履蘭Maudiae Type品系之組培苗適合栽培於玉米穗軸、花生殼、香菇廢棄太空包混合比例為：2:0:1和0:3:1之介質，苗期則以栽培於2:0:1、0:2:1和對照組之植株生育表現較佳。春石斛Dendrobium Lai’s Yukisakura品種，以不同替代性介質配方處理之植株根系於換盆初期褐化，影響後續植株之生長，與對照組相比呈現顯著的生長弱勢。文心蘭Oncidium Wildcat品種以栽培於100%香菇廢棄太空包介質之植株各性狀綜合表現最佳；Oncidium Rosy Sunset品種則以100%玉米穗軸、100%香菇廢棄太空包、2:0:1、1:2:0和對照組5種介質處理較佳。報歲蘭以1:4:2和1:2:4之介質處理對植株生長較佳；四季蘭則以1:2:4之介質處理植株生長較佳。 In order to seek replacement materials for cultivation of orchids seedling this research uses mixed substrates of different ratio corn cobs peanut shells and mushroom waste sawdust. Results showed that young plant of Paphiopedilum were planted in mixed substrates at mixing ratio of 2:0:1 and 0:3:1(corn cobs: peanut shells: mushroom waste sawdustv/v/v) were better than other treatments. The optimal substrates for seedlings of Paphiopedilum planting were controls treatment and mixed substrates at mixing ratio of 2:0:1 and 0:2:1(v/v/v). Using mixed substrates cause root-browning and slow growth of Dendrobium seedlings.Seedlings of Oncidium Wildcat growed well by using 100% mushroom waste sawdust as substrate. Seedlings of Onc. Rosy Sunset were planted in mixed substrates at mixing ratio of 2:0:1 1:2:0 (v/v/v) 100% corn cobs 100% mushroom waste sawdust and control treatment were better than other treatments. The optimal substrates for seedlings of Cymbidium sinense were mixing ratio of 1:4:2 1:2:4 (v/v/v) and the optimal substrates for seedlings of Cymbidium ensifolium were mixing ratio of 1:2:4(v/v/v).According to the results it is feasible that using corn cobs peanut shells and mushroom waste sawdust to cultivate Paphiopedilum2014 種子(苗)有害生物診斷鑑定技術、檢驗技術驗證及防治技術之研究與應用 Establishment and Research of Seed andSeedling pathogen detection protection Techniques 103農科-10.2.2-種-X1 103AS -10.2.2-SS-X1 繁殖技術課 Propagation Technology Section 邱燕欣 Yen-Hsin Chiu a.符合目標市場外銷國之種子苗驗證技術盤點與研發：發展標準化之馬鈴薯種薯病害採樣檢測流程符合健康種薯生產驗證之應用。本年度為發展馬鈴薯軟腐病與青枯病之免疫螢光檢測法。b.豇豆種傳病害滅菌處理技術之研究：開發有效的豇豆種子滅菌處理方法，降低豇豆種子攜帶萎凋病菌之機率，減少田間豇豆萎凋病之傳播與發生。豇豆種子所攜帶之豇豆萎凋病菌（Fusarium oxysporum f. sp. tracheiphilum(FOT)）能藉由污染種子表面或侵入種皮內部而進行傳播。本研究調查結果顯示，分別在屏東白仁白皮、紅花仁淡青皮、紫仁花莢豇豆與黑仁青莢目豆上檢測到FOT的存在，種帶FOT比率為3~25%。其他真菌與細菌的種帶比率也相當高，經表面消毒後雖可有效減少部分真菌與細菌的帶菌率，但仍有存在於種皮內部的可能性。利用殺真菌劑進行種子滅菌處理結果顯示，因種子自然攜帶FOT的比率偏低且不穩定，無法有效確定藥劑的滅菌效果。c.草莓病害非農藥防治技術開發：c.-1 草莓病害非農藥防治技術開發：研發適用於防治草莓炭疽病的非農藥製劑，以減少化學農藥的用量，降低農藥殘留對人體健康與環境生態的危害風險。結果顯示(1)於草莓組培培養基中施用不同濃度甲殼素，出瓶定植於介質後，接種炭疽病原菌，添加甲殼素濃度至100ppm時，炭疽病病斑偏小，而添加甲殼素其他濃度時則無差異。(2)利用不同濃度甲殼素處理草莓走莖苗防治草莓炭疽病的效果，結果顯示處理不同濃度甲殼素之草莓走莖苗初期罹病率較對照組低。比較草莓走莖苗接種炭疽病前摘葉與不摘葉處理，結果顯示接種炭疽病前未處理摘葉較處理摘葉之死亡率低。c-2 自台灣中南部及東部地區田土中分離出26株耐熱微生物，經對峙培養篩選出5株拮抗微生物，於PDA上可抑制草莓炭疽病菌落生長達44.62%以上； 5株拮抗微生物培養24小時的菌液即可抑制病原菌孢子發芽或發芽管生長。在生物防治試驗，刺傷接種炭疽病菌前草莓葉片處理拮抗微生物培養液， 1週後以編號B2014-12與21拮抗微生物可使病斑較對照組略小；刺傷接種後處理拮抗微生物編號B214-12亦可減少16%病斑面積。噴灑炭疽病菌孢子懸浮液接種前連續3天處理拮抗微生物菌液，以B2014-12處理較對照組罹病度減少2.3-4%，市售生物農藥則以Bs最具防治效果，可減少植株罹病度4.3-12.5%。d. 抗病毒血清製備技術之開發與利用：無性繁殖體在進行組織培養大量繁殖前，進行標的病害的檢測可確保繁殖體的健康，為生產健康種苗之基石。組織培養的標的病害包括病毒病、類病毒病以及菌質體病害，多以enzyme-linked immunosorbent assay (ELISA)、PCR(reverse transcription-PCR RT-PCR)，作為檢測植物病毒病之技術，其中ELISA仍是主要檢測植物病毒病之技術，血清為此技術之重要成本，因此本計畫擬以大腸桿菌表現轉殖之重組蛋白，作為生產標的病毒之蛋白來源，生產重要作物之檢測血清，本計畫於完成葡萄病毒A(Grapevine Virus A)之抗血清之生產80ml，力價稀釋可達8000-16000倍。 a. Development of standardized sampling and testing of the potato disease potato production processes comply with health applications verified.b.The objective of this program is to develop a convenient and effective seed disinfection technique on asparagus bean for decreasing the ratio of seed carrying Fusarium oxysporum f. sp. tracheiphilum. This disinfection technique will be helpful for diminishing the transmission and occurrence of the Fusarium wilt of asparagus bean in the fields.The Fusarium oxysporum f. sp. tracheiphilum (FOT) carried by asparagus bean seeds could be transmitted by polluting the seed surface or infecting into seeds. The investigation results showed that the FOT was detected on four asparagus bean cultivars with ratio of 3-25%. Other fungi and bacteria were also detected with high seed-carried ratio butafter surface disinfection the ratio of some fungi and bacteria decreased effectively. However it was still possible that the fungi and bacteria existed in seeds. The result of disinfection with fungicides indicated that the effect of chemicals were not able to be confirmed yet owing to FOT-carried ratio on asparagus seeds were low and not stable.c.c-1 Strawberry disease non-pesticide control technology development :research for prevention and treatment of strawberry anthracnose nonpesticide formulations to reduce the amount of chemical pesticidesreducing pesticide residues on human health and the environment and ecology of the risk of harm. (1)Application of different concentrations of chitin in strawberry tissue culture medium. Processing plants 100 ppm concentration of chitin its anthrax lesion smaller and the processing of chitin concentrations no other impact.(2) Strawberry seedlings treated with different concentrations of chitin treatment after picking leaves and do not pick the leaves inoculated with anthrax bacteria. Strawberry seedlings treated with different concentrations of chitin its initial investigation of anthrax lower disease severity compared to the control .The results showed that treatment plants do not pick off the leaves and leaf compared the results do not pick leaves treatment had lower mortality. c-2 26 heat-resistant bacteria were isolated from field soil collected in central2014 耐濕冷馬鈴薯品種選育 Wet and cold resistant breeding for potato 103農科-14.1.2-種-X1 103AS -14.1.2-SS-X1 品種改良保護課 Plant Breeding Section 張勝智 Sheng-Chih Chang 1. 異常的氣候，常是造成農民栽培作物歉收的主要原因之一。近幾年的氣候變遷，尤其是溫度與雨量的變化，均間接或直接造成生產的損失。馬鈴薯在台灣主要於冬季種植，但近年來的異常氣候，如低溫與多雨的異常氣候，常伴隨發生，導致馬鈴薯植株更易腐敗且多種病害發生嚴重，尤其是大規模晚疫病所造成的減產問題，故栽培者常需噴濕大量農藥防治與增加管理負擔，除影響農民收益外，更造成生產成本增加、食物安全與市場價格波動劇烈等問題，造成嚴重影響。因此為改善低溫高濕造成的多種問題，故以品種改良為目標，針對本場經多年選育具優良生長勢、產量與品質的48個營養系，進行低溫高濕的汰選評估。2. 經100/101年期的汰選，初選出11個具耐濕與高產生育特性的營養系(628、474、470、91A179、320、183、578、91N80、317、T75、462)，並於101/102年期持續汰選出5個分別(營養系470、628、91N80、320、462)具耐濕冷、生長勢強與高產，並具有中高耐病性(耐晚疫病)等特性之營養系，於102/103年再汰選評估出最優的3個營養系，分別為628、470與320，準備於103/104年期進行性狀調查，挑選表現最佳的營養系供作未來因應低溫高濕氣候的應用品種，期許能協助種苗業者克服因異常環境造成的減產與過量用藥等，所造成的食物安全與農民健康等問題。 1. Abnormal climate is often one of the main causes of farmers planting the crop failure. In recent years climate change the production of crops were especially caused loss by temperature and rainfall changing.Potato was mainly planted in winter in Taiwan. Anomalous Climate in recent years such as low temperature of climate anomalies was often associated with rainy. Those factors leaded to the potato corruption and induced more diseases. Especially large-scale production problems caused by late blight so growers often need to spraying pesticides to control and increase the burden. In addition to affecting farm incomeit also result in increased production costs food safety and market price volatility and other issues. Therefore in order to improve a variety of problems by low temperature and high wet we have been improved varieties by the goal. We select excellent growth potentialyield and quality of 48 clones after years of breeding and we select and assess by low temperature and high humidity for 48 clones.2. Those clones were selected on 2009 to 20102014 應用綠肥營造環境親和型水旱田輪作模式 The establishment of environment compatible crop rotation model on paddy field using green manure cr 103農科-14.1.3-種-X1 103AS -14.1.3-SS-X1 種苗經營課 Seed & Seedling Management Section 鄭梨櫻 Lee-Ying Cheng 為調整連續二期水稻後再栽培馬鈴薯之耕作制度，設計二項含水稻、綠肥及馬鈴薯之不同輪作制度，利用綠肥效益建立嘉義地區馬鈴薯環境親合型水旱田輪作模式。103年一期及二期作試驗結果，一期作輪作綠肥太陽麻及二期輪作青皮豆皆可增加土壤有效性氮含量。二項輪作綠肥模式以輪作模式B收益較高，惟三項模式之全年收益仍待進一步評估。 To adjust the cropping of rice after two consecutive potato cultivationdesign binomial different cropping systems of rice green manure and potato contain use of green manure benefits established environment friendly water upland crop rotation patterns. The first year test results a sun hemp or soybean as green manure crop rotation can increase the effectiveness of the nitrogen content of the soil. Second term green manure crop rotation patterns could had higher rotation benefits but full-year earnings of the three models have yet to be further evaluated.2014 組織培養節能設備及技術之開發 Energy-saving equipment and technology development for tissue culture 103農科-14.1.3-種-X2 103AS -14.1.3-SS-X2 繁殖技術課 Propagation Technology Section 文紀鑾 Chi-Luan Wen 不公開 Not public2014 健康種苗生產供應與健康管理體系之建立 Establishment of health seedlings production and health managementsystem 103農科-14.2.2-種-X1 103AS -14.2.2-SS-X1 繁殖技術課 Propagation Technology Section 羅英妃 Ying-Fei Lo 本年度進行一般芽菜與安全芽菜生產流程分析、成本分析，並於06月26日舉行安全芽菜生產技術示範觀摩會，經由成本分析及問卷調查顯示，目前安全芽菜售價偏高為反應成本，於各場展覽會場安全芽菜的產品通路詢問度高，顯示消費者對於安全生產的重視性提高。本試驗開發馬鈴薯台農一號種基本種薯與克尼伯種原種薯非農藥防治管理技術，生產之種薯品質與傳統栽培無差異。食用薯生產方面，使用認證健康種薯並以傳統農藥管理產量較佳，單株產量達730 g，但非農藥防治管理可減少化學藥劑使用之風險，提供消費者食用安全。豇豆為台灣夏季重要蔬菜之一，近年受到病毒病及萎凋病影響栽培面積漸漸減少，利用商業品種嫁接耐萎凋病根砧可有效減少萎凋病發生，為了解豇豆耐萎凋病嫁接苗之最適肥培管理模式及土壤有益微生物對其生育之影響，本年度於栽培期使用不同施肥量及施用不同有益微生物並調查豇豆耐萎凋病嫁接苗之產量及品質，試驗發現使用N:P2O5:K2O施用量12:10:10公斤/分地時豇豆耐萎凋病嫁接苗果莢數為594、總產量18.6公斤為最高，且略高於對照組（’三尺青皮’自根苗），使用豇豆耐萎凋病嫁接苗不需增加施肥量即可提升35%-47%之產量，每公頃可增加新臺幣154303-207018元收益；有益微生物部分，枯草桿菌及木黴菌的施用對豇豆耐萎凋病嫁接苗之果莢數、總產量、單果莢鮮重及果莢長度無顯著影響，可能與處理時期較晚及施用化學肥料有關，試驗結果顯示有益微生物的施用對豇豆耐萎凋病嫁接苗生產助益較小。 We compared the cost and market sold prices of sprout production procedures between the traditional and safety methods. The meeting of safety sprout production be held on 26th June this year. Participates showed their high interesting to this program. Even the prices of safety sprout products are not as cheap as traditional products. The safety issues of sprout still are concentrated by customers. In this study we developed the non-pesticidal and non-fungicidial management techniques of ‘Tainon No.1’ basic seed potato (G1) and ‘Kennebec’ elite seed potato (G3). Compared non-pesticidal and non-fungicidial management with traditional management the quality of seed potato were no significant difference. In table potato production period there are more yields to select certified seed by traditional management and the yield of single plant is 730g. However we can reduce the risk of using chemistry by nonpesticidal and non-fungicidial management and provide a food safety choice to customer. Asparagus bean is one of the important summer vegetables in Taiwan. Virus and Fusarium wilt result tin decreasing of cultivation area in recent years. Asparagus bean grafting Fusarium wilt resistant seedlings can efficiently reduce Fusarium wilt occurred.In order to study the suitable fertilizer managements and also the effect of effective micro-organisms on Asparagus bean grafting Fusarium wilt resistant seedlings’ development and yield different fertilizer managements and effective micro-organisms were used in the trails.120:100:100 (N:P2O5:K2O) kg/ha had the highest pod number (594) and total yield (18.6 kg). Compare to the CK total yield of asparagus bean grafting Fusarium wilt resistant seedlings increased 35%-47%. Effective micro-organisms had no significant effects on pod number2014 設施苦瓜栽培技術之研究 Study on cultivation technology of bitter gourd in protected facility 103農科-14.4.1-種-X1 103AS -14.4.1-SS-X1 品種改良保護課 Plant Breeding Section 郭宏遠 Horng-Yeuan Kuo 本年度進行抗萎凋病絲瓜及南瓜根砧評估、萎凋病拮抗微生篩選、設施內苦瓜物候調查、蜜蜂行為及蜂箱降溫研究。在萎凋病接種試驗方面，經剪根接種法汰選出具有優良抗性的絲瓜品系如96-157XL2 與96-111XR3及南瓜品系4與13，可作為萎凋病抗性根砧的汰選參考。嫁接親和試性驗方面，以絲瓜品系嫁接苦瓜’新社自留種-粗米’，顯示以95-153XL2品系具較好之砧穗親和力。以對峙培養篩選出3株拮抗微生物可抑制萎凋病菌落生長，具應用於萎凋病防治之潛力。設施內栽培之苦瓜，雌花產生節位提早7-8節，與露天栽培的果實生育日數相近，但種子量、果寬及單果重量均較少。蜜蜂在溫室內離返巢之趨勢相同，在一天內有兩次之高峰，分別為上午8點及下午2-3點間，當日開花之苦瓜雌花應在上午時段即由蜜蜂完成授粉。降溫處理III可使整天的蜂箱溫濕度均低於對照組。 Evaluation of resistance of sponge gourd and pumpkin rootstocks to Fusarium wilt selection of antagonistic microorganisms to Fusarium wiltphenology investigation of bitter gourd introduction of honey bees to help pollination of bitter gourd production and the effect of environmental factors and flowering performance on bee pollination behavior in the facilities were conducted this year. In the Fusarium wilt inoculation test two sponge gourd lines (96-157XL2 and 96-111XR3) and 2 pumpkin lines ( 4 and 13) were chosen for better resistance to Fusarium wilt which had the potential to be applied as rootstock for controlling Fusarium wilt of bitter gourd. In addition sponge gourd line-95-153XL2 showed better grafting compatibility. Three antagonists were selected by dual culture. The bacteria showed activities to suppress mycelia growth of Fusarium oxysporum f.sp. momordicae. They had the potential to be applied for controlling of Fusarium wilt. Bitter gourd grown in the greenhouse produced female flower at lower nodes than that in the open field for 7-8 nodes. However with the similar fruit growth period the fruits produced in the greenhouse were less seeds2013 植物種苗研究團隊-基因轉殖及非基因轉殖種苗驗證及共存體系之建構-基因轉殖作物認驗證與檢監測模式之建立 Construction of the certification and coexistence system for GM and non-GM seedlings 102農科-1.1.1-種-X1 102AS -1.1.1-SS-X1 生物技術課 Biotechnology Section 沈翰祖 Han-Tsu Shen 基因轉殖作物檢監測體系由農糧署委託種苗改良繁殖場邀集農業試驗所、桃園區農業改良場、台南區農業改良場、花蓮區農業改良場、國立中興大學等單位建立「基因轉殖作物檢監測小組」，藉由「檢測技術建立」、「儀器與檢測精準度試驗」、「檢測樣品檢出能力試驗」、「能力試驗」等，已累計建立基因轉殖木瓜2個、玉米10個、大豆3個以及馬鈴薯1個品項之標準化檢測模式，並通過「財團法人全國認證基金會（Taiwan Accreditation Foundation TAF）」之實驗室認證。本年度檢監測小組進行基因轉殖大豆、玉米3次能力試驗；並取得基因轉殖植物審議委員會委任為基因轉殖大豆與玉米之檢測機構。建立實驗室定量檢測量測不確定度分析模組，並以top-down策略完成基因轉殖大豆品項305423與基因轉殖玉米品項NK603之量測不確定度分析；以及使用bottom-up策略評估基因轉殖大豆品項305423之量測不確定度。已完成基因轉殖玉米與傳統農業共存栽培建議書與基因轉殖作物標準化取樣及監測模式，並以田間試驗模擬基因轉殖大豆花粉飄散趨勢，本年度春作試驗並未發生花粉污染之情形。已利用衛星定位(GPS)監測台灣水稻29個、油菜10個、馬鈴薯5個、玉米8個、大豆16個栽培區、野生大豆21個生長區等資料，且以地理資訊系統(Geographic Information System GIS)分析是否受到基因轉殖作物污染，並完成「基因轉殖作物混雜度調查資料管理系統」。 The detection and monitoring system of transgenic crops in Taiwan was entrusted by the Agriculture and Food Agency (AFA) Council of Agriculture (COA) and the team member is Agricultural Research InstituteTaoyuan District Agricultural Research and Extension Station Tainan District Agricultural Research and Extension Station Hualien District Agricultural Research and Extension Station National Chung Hsing University and Seed Improvement and Propagation Station (SIPS). Technique for detection of GM crops consists of four distinct steps including detection capability proficiency test the standard detection and monitoring model. The biological area of detection laboratory at SIPS has been accredited according to ISO/IEC 17025. There are several items to be accredited by TAF2013 植物種苗研究團隊-生技種苗檢測服務建置與產業推動 Industry extension and establishment ofbiotech seedling detection serviceplant seed/seedling team 102農科-1.1.2-種-X1 102AS -1.1.2-SS-X1 生物技術課 Biotechnology Section 鍾文全 Wen-Chuan Chung 利用multiplex PCR技術進行基因轉殖木瓜檢測，得知Multiplex PCR可取代一般PCR進行基因轉殖木瓜檢測，以達到降低檢測成本，並提升檢測能量之目的。評估SYBR Green、Safe view等2種螢光試劑對基因轉殖大豆與玉米檢測效能，結果顯示SYBR Green分出基因轉殖及非基因轉殖大豆和玉米的能力優於Safe view。訪視6家文心蘭組培場，瞭解其設備規模、生產流程及瓶苗出貨標準，並蒐集市售瓶苗進行調查，探討影響瓶苗品質之外部型態指標，包括葉數、根數、株高、根長、鮮乾重，分析810個樣品發現瓶苗間葉片數並無顯著差異，根數及根長則有較高的變異程度，推測可能是子瓶移植操作的影響，株高仍是較為簡便可靠的分級標準，初步結果建議以區分大中兩級為可出貨品質，小等級植株管理模式還需進一步評估。度拜訪16家蝴蝶蘭組培場，與經營者訪談組培設備、生產流程及瓶苗出貨標準。從11家組培場中蒐集12份蝴蝶蘭V3瓶苗，每份有6瓶子瓶，共計72份樣品。這些瓶苗經合格率調查、莖葉及根的數量與重量調查後，分為4個瓶苗品質等級。取樣品進行植體成份分析，其餘植株上盆栽植，並移到專業的蝴蝶蘭中小苗栽培場栽培到中苗。取中苗再進行合格率、莖葉及根數量與重量調查，並分成4個中苗品質等級，並進行中苗植體分析。優良蝴蠂蘭瓶苗為株型端正、葉型與根正常、三葉以上葉片、二條以上有效根、株重3公克以上。葉的氮素含量中等(乾重1.5-4%)，高量的鉀含量(乾重5%以上)、磷含量介於乾重0.4-0.8%之間，鈣含量0.4%以上，鎂的含量在0.15%以上。以逆轉錄聚合酶鏈式反應(revese transcription–PCRRT-PCR)檢測蘭園蘭花常見病毒齒舌蘭輪斑病毒(Odontoglossum ringspot virus ORSV)與東亞蘭嵌紋病毒(Cymbidium mosaic virus CymMV)，共檢測1346件瓶苗樣品，檢測結果得知健康種苗比例為92.8%。 Multiplex PCR and PCR was apllied to detect GM papaya seedlings.Multiplex PCR had the same result as general PCR resulting in cost reduction and enhancing detection capacity. We visit six Oncidium tissue culture factories to understand the company profile production processes and plantlets standards in this year. Furthermore we gather commercially Oncidium TC plantlets to explore the influence of the external traits of plantlets quality including the number of leavesroot number plant height2013 植物種苗研究團隊-番木瓜種苗七號全兩性株調控基因分析與產業應用 Analysis of regulation genes of allhermaphrodite papaya cv. TSS No.7 and its application on industry 102農科-1.1.2-種-X2 102AS -1.1.2-SS-X2 繁殖技術課 Propagation Technology Section 李美娟 Mei-Jiuan Lee 不公開 Not public2013 種子檢查技術研習 Study on seed testing technology 102農科-4.2.1-種-X1 102AS -4.2.1-SS-X1 種苗經營課 Seed & Seedling Management Section 許鐈云 Ciao-Yun Syu 本年度參與ISTA於2013年6月6~9日於土耳其安卡拉(Ankara)舉行，由土耳其國家品種註冊與認證中心(Variety Registration and Certification Center VRCC)承辦之種子潔淨度(Purity)及發芽(Germination)研習。研習內容包含種子潔淨度與發芽之原理、定義、統計方式、品保管理及實際操作等，同時並參訪土耳其國家品種登記與認證中心之種子實驗室。於2013年6月11~17日至土耳其安塔利亞(Antalya)參加第30屆種子大會之種子研討會(Seed Symposium)及技術委員會年會(Meeting of Technical Committees)，除更新種子檢查相關資訊外，並聽取涵蓋五大主題之各國種子研究者成果演講發表及近150篇之海報發表。 In 2013 one staff of TSS attended the ISTA seed purity and germination workshop and the 30th ISTA congress and seed symposium hold in two cities of Turkey. The workshop hold in Ankara included the definitionstatistics quality assurance and practical training of seed purity and germination and visiting the ISTA certificated seed laboratory. After the workshop attended the ISTA Seed Congress hold in Antalya included the Seed Symposium and Meeting of Technical Committees to update the information of seed testing and hear the posters and oral presentations included 5 subjects.2013 赴澳大利亞進行檢定技術研習與交流 Study and exchange of DUS test technology with Australia 102農科-4.2.1-種-X2 102AS -4.2.1-SS-X2 品種改良保護課 Plant Breeding Section 劉明宗 Ming-Chung Liu 澳大利亞植物品種保護主要是由澳大利亞智慧財產局(IP Australia)下之植物品種保護辦公室辦理。澳大利亞植物品種權架構主要依據1994年所訂定植物育種權利法案，並配合UPOV1991年之國際公約，所有植物種類包括真菌類、藻類、甚至轉基因植物皆在保護範疇內。每年受理案件約300-350件，申請植物品種保護之種類以觀賞植物為主，約佔70%，其次為農藝作物與果樹及蔬菜類。澳大利亞之植物品種保護架構較特別的是利用認證人員(Qualified person：簡稱QP)，藉由這些人員進行品種比較試驗、對照品種之選擇、資料收集、數據分析、品種描述等。為了確保認證人員的品質，由植物品種保護辦公室辦理植物品種保護訓練計劃，對植物品種權檢定等相關能力，進行訓練。經由完成訓練課程給予分數或其他報酬之安排，使他們受到認證，並可委任執行新品種檢定工作。Floral International Pty Ltd 位於雪梨，主要生產水耕玫瑰切花、東方型百合、天堂鳥與蝴蝶蘭為主。也是澳洲目前唯一有申請蘭花品種權之私人公司，此公司與台灣世芥蘭園合作，接續生產蝴蝶蘭植株販售。並代理世芥蘭園申請品種權，共申請四個品種權。Queensland Orchid Biotechnology位於凱恩斯，是由台商於10多年前移民開設，主要經營蘭花生產與零售，蘭花種類以蝴蝶蘭為大宗，其他尚有仙履蘭及稀有原生蘭等。由於昆士蘭省氣候以熱帶為主，台灣優良之熱帶水果品種，若欲往澳大利亞種植與生產，則位於北昆士蘭之凱恩斯是不錯選擇，因當地具飛機場且交通便利且氣候適宜。台灣若要發展與台灣氣候相反季節之農業生產地方，澳大利亞之昆士蘭省是不錯之選擇。 Australian Plant Variety Protection of Australian is mainly handled by the PVP office under the Australia Intellectual Property Office (IP Australia). Australian plant variety rights framework set in 1994 based primarily on plant breeders ' Rights and the International Covenant on UPOV1991 years with all kinds of plants including fungi algae and even transgenic plants are within the scope of protection . Accept cases about 300-350 pieces per year for the type of plant variety protection to ornamental plants accounting for about 70% followed by agronomic crops and fruit trees and vegetables . Australia 's plant variety protection structure is more special is the use of certified person (Qualified person: short QP) carried out by these qualified persons Variety Test control variety of selection data collection data analysis2013 青年農民植物種苗類別訓練成效追蹤評核之研究 The effectiveness assessment research of category training in seedling for young farmers 102農科-5.2.2-種-X1 102AS -5.2.2-SS-X1 技術服務室 Technical Service Section 鍾依萍 I-Ping Chung 種苗產業具有技術、資本密集之特性，近年來種苗業者技術層次不一，影響種苗品質、收益與產業發展，辦理種苗技術訓練以提升其技能。本國農民高齡化，需培植年輕人接手投入農業工作，規劃各類種苗業所需之訓練課程，並以參加農民學院植物種苗類別之結訓與在訓學員為對象進行訓練成效考評，建構適合青年從農訓練之模式，以提升從農之經營能力。一、研究方法(一) 結訓學員訓練成效追蹤調查以參與100-101年度農民學院植物種苗類別初階班(含)以上之結訓學員，透過郵寄問卷方式進行1.基本資料、2.訓練績效評估兩部分之調查。目的為瞭解學員經培訓後，目前從農情形及培訓對其生產經營管理能力是否有助益。(二) 在訓學員訓練成效與滿意度調查以參與本(102)年度農民學院植物種苗類別各訓練階段之學員，透過現場發送問卷方式進行1.訓練前測-能力認知調查、2. 訓練後測-能力認知與訓練滿意度調查。目的為瞭解學員經培訓後，各項能力是否有所提升以及其滿意度為何。二、結果與分析(一) 結訓學員訓練成效追蹤調查1. 基本資料108份有效問卷中，男性佔86%；年齡以51~60歲較多佔35%；教育程度以大專(學)院校佔56%；目前有從農者佔90%，從農年資以1~10年最多佔53%；有89%學員持有土地，其土地權屬自有佔76%、租賃佔24%；主要生產作物為其他(景觀苗木、中藥作物、檳榔…等)佔31%、蔬菜佔30%；經營方式主要是獨資佔51%；高達53%有僱工，並以短期僱用為多佔62.5%；有77%尚未通過任何認(驗)證。有關參加農民學院對農產品銷售額是否有增加，48%的學員認為沒有提升，40%認為提昇1-2成；而參加農民學院在農業經營利潤(收入－成本)方面是否增加，49%的學員認為有增加1-2成，42%認為没有增加。2. 訓練績效評估以九大構面進行平均值計算，結果以「農業政策」分數最高為4.06，「人力資源管理」次之為3.99，再者以「研發能力」3.88為第三高，而「社會參與」則為最低分3.44。(二) 在訓學員訓練成效與滿意度調查1. 訓練前、後測之能力認知調查各班別訓練前、後測分數相差最多前三名之項目，發現近9成為「生產管理」構面，相差最少之項目大部分集中在「社會參與」構面。2. 訓練後滿意度調查各班別之訓練後滿意度調查，13個題項各班滿意度平均值皆有4以上。三、結論(一)結訓學員訓練成效追蹤調查青年農民(A)其農場經營方式有35%是家族經營，並有58%是僱用長期工，經營的土地面積有50%是介於0.5-3公頃；與45歲以上農民(B)的經營方式有明顯不同，B是以獨資(62%)、僱用短期工(76%)、土地面積大多為0.5公頃以下(44%)。推論來受訓的青年農民有35%的學員為接手家族企業而投身農業，因家族產業已建置一定規模，故其土地面積較B大，並以長期僱工的方式經營。此外，藉由t test檢驗青年農民(A)與45歲以上農民(B)對於訓練成效之認知，結果發現在「行銷管理」、「財務管理」及「資訊管理」此三構面共5個項目上，A的能力提升認知均顯著低於B(p＜0.05)，推判A對於農場經營較有整體性的重視。(二) 在訓學員訓練成效與滿意度調查本(102)年度進行在訓學員共調查6班別8梯次之訓練班，在能力認知上訓練前與後分數差異較多的題項，近9成落在「生產管理」構面，而差異較少的題項，則多落於「社會參與」構面。並發現實習或參訪時數較多之課程，學員的認知能力提升亦相對較高。各班別各題項滿意度總平均值皆達4以上，表示本年度的參訓學員對本場所開設之訓練課程感到滿意。 The aim of the investigation is to evaluate the trainees who are participating and had completed the plant seeding training courses in the farmer’s academy for establishing appropriate training pattern of young people from rural and improving operational ability of agriculture.Therefore we are mainly aimed at the trainees who graduated in the years 2011 to 2012 and participated in 2013 to be the respondents in this investigation. Furthermore questionnaire is the primary method of investigation and it divided into two dispatch ways which are by letter and in person to the respondents.1. The postal questionnaire is designed for the trainees who had completed the plant seeding training courses from the level of beginners to high-level in the farmer’s academy during the years 2011 to 2012.Moreover this survey is to understand the situation of working in rural and the training performance evaluation from the respondents.2. Additionally in the first and the last day of the plant seedingtraining courses in the farmer’s academy 2013 we also prepared and offered questionnaires to each level of trainees in order to perceive any difference in their cognitive ability during the period of training.Besides the respondents reply comments on the questionnaire so as to realize their opinion about this course. There are 35% of young rural trainees devoting to the farming because of inheriting family business. In addition2013 建構亞太植物種苗產業服務資訊平台 Construction for Asia Pacific Seedlings Industry Services Platform 102農科-7.1.1-種-X1 102AS -7.1.1-SS-X1 技術服務室 Technical Service Section 周明燕 Ming-Yenn Chou 亞太植物種苗產業服務資訊平台共累積410137筆資料，瀏覽人次204238人次，隨本平台之宣傳行銷，呈現現逐月成長趨勢；功能使用上多集中在最新消息與產業服務兩大單元，顯見目前本平台之知悉度仍低，宜更積極增取平台曝光率。透過技術盤點了解目前國內外相關技術之重點與知識缺口，其分析結果除可供國內相關技術研發考量外，現以「產業分析專欄」與「重要專利情報」兩種形式，供亞太植物種苗產業服務資訊平台會員市場面之參考。未來預計應用於技術主題之系列教材、社會網絡及文字探勘等平台加值服務。蝴蝶蘭品種性狀影像辨識系統為協助提升植物品種智慧財產權性狀檢定效能之輔助工具，目前已輸入350筆品種影像資料，隨著資料量越多，系統之辨識效能也會隨之提升，對業務效能之協助提升也更具助益。 "Pacific plant seed industry information platform for a cumulative total of 410137 documents visits 204238 people. Function uses the latest news and more concentrated in two industrial services unit which shows the current platform is still aware of the low should take a more active growth platform for exposure.Inventory through technical understanding and knowledge gaps in the current focus on domestic and foreign technology in addition to its analysis of the results of research and development of related technologies available for domestic considerations the present in two formsindustry analysis column"" and ""important patent information"" for the Asia-Pacific plant seed industry market information platform serving member of the reference plane. Future technologies are expected to be applied to the theme of the series of textbooks2013 RFID技術應用於種子倉儲管理之研究與開發 Study and development on RFID technology for the seed storage management 102農科-7.3.1-種-X1 102AS -7.3.1-SS-X1 種苗經營課 Seed & Seedling Management Section 許鐈云 Ciao-Yun Syu 種苗產業可包括種子產業及種苗產業，其中種子產業所佔的比值大於種苗產業，且種子的生產銷售亦較鮮果複雜許多。本場擁有9座種子冷藏庫，總計700坪冷藏庫設施，倉儲容量約260萬公斤，長年配合政府政策負責供應全省雜糧、綠肥及蔬菜作物種子，操作從契作生產及外購種子、調製、加工、倉儲、運輸至推廣銷售等一系列種子產銷流程作業。100年度目標針對種子倉儲部分建構「種子倉儲管理系統」部分，並結合無線射頻辨識(RFID)應用，達成種子倉儲流程作業電子化。去(101)年度第二期開發案擬擴大應用層面，於軟體系統方面將第一期開發的系統加以擴充，增加會計(含出納)功能。今(102)年度在種子供銷管理導入二維條碼技術，希冀建立完整二維條碼(QRCode)種子供銷鏈，並擴大系統功能建置，以前兩年系統為基礎，增加銷售管理部分功能。 Taiwan seed improvement and propagation station (TSS) has 9 seedrefrigerated storages and is responsible for the seed production of cereal green manure and vegetable. Moreover there are a series of seed producing and marketing procedure operation including producingpurchasing processing packaging storage transportation and marketing.In 2011 it’s aim to accomplishment of the efficient method of seed storage management by RFID technology in order to maintain the seed of quality to decrease the waste of manpower and time and to enhance the check time of the inventory in the seed storage effectively. In 2012it’s important to extend the capability of the system including accounting and cashier. In 20132013 植物種苗研究團隊-番茄抗病與花椰菜自交不親和性分子標誌建立與應用 The establishment and application of specific molecular markers for tomato and cabbage traits 102農科-9.1.1-種-X1 102AS -9.1.1-SS-X1 生物技術課 Biotechnology Section 孫永偉 Yung-Wei Sun a.番茄抗萎凋病基因型之分子鑑定：番茄萎凋病為重要真菌性病害，本試驗建立multiplex PCR (含I-2與Fusarium oxysporum)共顯性分子標誌(I2/Fu)，可同時檢定番茄抗萎凋病與病原菌基因。利用此分子標誌可擴增抗萎凋病基因700或852 bp之DNA條帶、擴增感病基因2320 bp之DNA條帶、擴增萎凋病(race2)基因1000 bp之DNA條帶。此分子標誌檢測結果與國內育種者或種子公司已知抗感性品種吻合。b.花椰菜自交不親和性基因型之分子鑑定：利用花椰菜常用之7個育種親本品系為試驗材料，以Brace等人在1994年建立之PCR-酶切試驗分類結果為依據，進行具識別性SCAR引子對的篩選，七個品系各6個單株材料先經PCR-酶切檢驗，各品系每個單株間並無差異性，而各品系的自交不親和基因型分類結果僅SP04與SP06為相同類型。而後進行花椰菜SCAR專一性引子的篩選，經PCR試驗結果後發現，有2組SCAR引子：D4-1*D7-2可鑑別品系SP01、SP04/SP06、SP08;K1-2*K4-4可鑑別品系SP07、SP08、SP09、SP10。c.番茄抗菸草嵌紋病毒分子標誌之建立：菸草嵌紋病毒為菸草鑲嵌病毒屬(Tobamovirus)，為番茄三大病毒之一。本試驗針對抗病基因(Tm-2)與病毒各開發專一性分子標誌(Tm2與TMV)。目前已開發一組共顯性分子標誌，可同時擴增番茄抗嵌紋病毒基因(Tm-2或Tm-2 2) 650 bp之DNA條帶、擴增感病基因(tm-2) 450 bp之DNA條帶。本研究與亞蔬中心合作開發一組分子標誌可檢測臺灣常見3種嵌紋病毒strains (TMV-0、TMV-1、TMV-2)，可擴增3.0 kb之DNA條帶。上述分子標誌可協助育種者早期篩選抗病植株、確認抗病基因型(R/R、R/S、S/S)及病毒感染情形，提高育種效率。 The projects of this program have three components including. The first is the establishment and application molecular markers of the resistance to Fusarium oxysporum in tomato analysis system. Specific markers I2 was developed for differentiating allelic variation at I-2 locus. It amplifies fragments from both resistant and susceptible tomato accessions with different molecular sizes that corresponding to the presence of I-2/I-2 I-2/i-2 and i-2/i-2 alleles. This study we also merged primer set I2/Fu as Multiplex-PCR through once PCR detecting I-2 i-2 andFusarium oxysporum simultaneously. Primer I2/Fu could amplify resistance genes I-20.7 or 0.8 kb susceptibility gene i-2 2.2 kb and Fusarium spp. 1.0 kb of DNA bands. The molecular markers could help breeders early screening and confirmation disease resistant plant genotypes helping to accelerate the breeding and promotion of disease-resistant tomato lines.Using seven cauliflower lines which are commonly used breeding parental as test material. To do SCAR primers screening based on Brace’s PCRrestriction classification test results that established in 1994. The result of PCR-restriction classification in addition to the line SP04 and SP06 are same self-incompatibility genotype the other are different.Then the SCAR makers screening results showed that there are two sets of SCAR primers: D4-1 * D7-2 can identify lines SP01 SP04/SP06 SP08; K1-2 * K4-4 can identify lines SP07 SP08 SP092013 植物種苗研究團隊-作物種苗微體繁殖技術之開發與改進 The study of healthy seedling production by tissue culture 102農科-9.1.1-種-X2 102AS -9.1.1-SS-X2 繁殖技術課 Propagation Technology Section 陳哲仁 Jen-Ren Chen 本研究目的為探討綠竹(Bambusa oldhami)芽體形成，以1 mg/L BA＋1 mg/L kinetin處理之側芽培植體形成叢生芽體最高約為85.1%，培養3週後已有不定芽形成。而不定芽形成率則以添加1 mg/L BA＋3 mg/L kinetin最高約為96.2%。在不定芽誘導發根試驗結果顯示以1.0-2.0 mg/L NAA或IAA之發根率94.1-95.8% 為最佳，平均根長約有1.8cm。透氣式培養容器在組織培養對植物生長與發育是很重要因子，下一個研究為利用三種不同型式之培養容器器對丹蔘發根之影響，使用在MS基本培養基中培養之叢生芽體，培植體來自側芽，在發根培養基中，使用透氣式塑膠培養瓶提高發根率(98.2%)、葉長(5.2 cm)、根數(2.37)、根長(4.1 cm)及最長的根(5.8cm)。本試驗目的在於了解不同培養基成分對春石斛組織培養之芽體增殖階段和根系誘導階段瓶苗品質之影響。由試驗結果得知，增殖階段組培苗之株高、葉數、葉長、葉寬、芽體增殖數和褐化率皆受到培養基中MS培養基、馬鈴薯粉和蔗糖三種成分濃度以及其交感效應之顯著影響。發根階段則主要受到MS培養基、香蕉粉、馬鈴薯粉和蔗糖濃度四種成分之主效應及含NAA在內五種成分彼此間之交感效應所影響。而經分析，於增殖階段以全量MS培養基加上4g/L馬鈴薯粉和20g/L蔗糖之處理組合最佳，根系誘導階段則以1/2MS培養基加上0.1mg/L NAA、30g/L香蕉粉、4g/L馬鈴薯粉和20g/L蔗糖之處理組合最佳。種苗金皇一號石斛冷水萃取物可以活化小鼠肺巨噬細胞(RHS cells)且促進NO的釋放。 The objectives of the present study were to develop culture method for shoot formation in bamboo (Bambusa oldhami). Treatment of 1 mg/L BA＋1 mg/L kinetin was found best in multiple shoots induction from lateral bud explants induction percentage reached 85.1% shoots were formed after culture for 3 weeks. The highest percent shoot formation which is 96.2% was found in the treatment of 1 mg/L BA＋3 mg/L kinetin.In addition treatment of 1.0-2.0 mg/L NAA or IAA was found best in root formation from shoot formation percentage reached 94.1-95.8% the average roots Length formed per shoot were 1.8cm。Vented vessel is an important factor effective on plant growth and development in micropropagation. The next present study describes the effects of different vessel (plastic vented vessel glass flask sealing with aluminum foil and glass flask sealing with Rubber stopper) on some rooting characters of Danshen (Salvia miltiorrhiza) using multiple shoots cultured on MS basal medium. Explants were lateral shoots. In rooting media with plastic vented vessel was more effective. Maximum percentage of rooting(98.2%) leaf length (5.2 cm) root number (2.37) root length (4.1 cm) and longest root length (5.8cm) were observed in explants treated with plastic vented vessel.We study the effects of additives in vitro culture on quality of plantlets Dendrobium. The results indicated that using MS medium supplemented with 4 g/L potato powder and 20 g/L sucrose are optimal for shoot proliferation. Rooting medium of the best is using 1/2MS medium supplemented with 0.1 mg/L NAA2013 植物種苗研究團隊-仙履蘭產業技術開發及應用 Paphiopedilum industry technology development and application 102農科-9.1.1-種-X3 102AS -9.1.1-SS-X3 生物技術課 Biotechnology Section 廖玉珠 Yu-Ju Liao 不公開 Not public2013 建立飼料玉米種子籌供體系 Establishment of hybrid corn production and supplying system 102農科-9.2.1-種-X1 102AS -9.2.1-SS-X1 農場 Research Farm 陳學文 Hsueh-Wen Chen 計畫於台中市新社區及屏東縣麟洛鄉兩地，以台農1號及台南24號玉米兩品種，以不同去雄方式對玉米採種之影響，用採種田春、秋兩季之栽植方式，探討其對玉米穗結實及產量之影響，以作未來量產模式之參考。尋找參試品種新採種栽培及調整採種適期以適應氣候變遷，建立量產模式，穩定生產種子，以配合政府政策，生產所需種子。搜集國際商用飼料玉米品種，評估各品種於台灣之適應性，建立適宜台灣種植之國際飼料玉米品種資料庫，期能提供農民多樣選擇並穩定飼料玉米種子供應。調查雜交玉米F1種穗及種子於乾燥過程中水分含量變化與大氣環境和耗油量之關係對調製作業之影響，掌握調製作業時程，建立雜交玉米種穗在不同乾燥環境之調製作業模式，因應環境氣候變化，適時調整乾燥作業模式，提高調製作業效率，達到節省能源目標。 We conducted different planting dates for Taichung area for spring and autumn corp. Influence by different detasseling methods on seed production of corn.Investigation of adaptability to environment and growth characteristics and yield as reference for future production models. Introduction of international commercial corn varieties will be evaluated the adaptability and appropriate corn variety databases in order to provide diverse selection and stability in feed corn seed supply. Investigation of hybrid corn F1 seed and moisture content during the seeds in a dry atmosphere environment and the relationship between fuel consumption and the changes to modulation effect of job and control different drying operation mode of environment and climate . We hope that can improve efficiency to save the energy.2013 果樹研究團隊-優質番木瓜品種選育、採種及栽培技術改進 Good quality varieties selection and improvement the technology ofcultivation and seed production on papaya 102農科-9.2.2-種-X1 102AS -9.2.2-SS-X1 屏東種苗中心 Pingtung Seed & Seeding Research Center 邱展台 Chan-Tai Chiu 本年度擬進行優良耐儲運品系f6選拔，進行雜交，供選拔優良組合進行後續品系比較試驗。另種植果皮光滑，生理性斑點少之品系與全兩性株品系之雜交第三代，供下年度分離選拔具全兩特果皮光滑品系。以優良純系利用秋水仙素誘導產生四倍體品系，再與2倍體雜交產生3倍體雜教交一代品系，供後續觀察，藉以育成雜交一代無子籽的番木瓜品種。耐儲運品系選育，以引進自東南亞的品系與本場育成之品系為雜交，經6代選拔之選拔純化，共選出14個品系，14個品系均為紅色果肉，果重412-758公克，果長13-18.8公分，多為西洋梨形之中小型果，果實總可溶性物11.9-14 % Brix ，以102-I-31、102-I-44、102-I-51等3品系之果肉硬度較高，102-I-18、102-I-28、102-I-36品系之果肉較軟。果皮光滑品系選育系以Golden為親本，其雜交一代的果皮生理性斑點仍相當多，經第3代分離，共選出12株果皮較光滑的特性。著果數47-141粒果重302-951公克，總可溶性固形物10.9-14.5 % % rix，果實品質優良。4倍體的植株，無法產生花粉，雜交只收穫1果實，但無種子，經自交授粉亦只收穫1果，獲得少許種子，這些種子已完成播種，在檢查染色體數目後，再行定植。 The objectives of this study are planting good resistance to storage and transportation lines hybrids for selecting good combinations for subsequent .comparison test. Plant Peel smooth physiological spotted little of all hermaphrodite strain providing isolation and selection for next year. Tetraploid plants were induced by colchicines then crossed with diploid plant to produce hybrid triploid plants. Charactersof F1 hybrid variety triploid plants will be observed.Breeding program of resistance to storage and transportation was conducted with introduce strains from Southeast Asia. Through 6 generationssegregation and purification 14 lines were obtained. All 14 strains are red pulp medium sized fruit of the pear-shaped high sweetness. Four lines’s fruit have higher firmness and the flesh softens speed slower than the sunrise and the Tainoun No2. Peel smooth breeding utilize Golden for the parents after separation of the 3nd generation were elected the 12 line of Peel smooth characteristic2013 植物種苗研究團隊-果菜類作物品種改良及採種技術改進 Study on improvement of fruit vegetable varieties and seed production technology 102農科-9.2.2-種-X2 102AS -9.2.2-SS-X2 品種改良保護課 Plant Breeding Section 郭宏遠 Horng-Yeuan Kuo 本計畫之目的在育出高產量、高品質、耐熱和抗病之胡瓜、南瓜、苦瓜、番椒及茄子之新品種，並進行具番茄花粉保存及克服彩色甜椒高溫採種問題之研究。胡瓜育種目標為鮮食品質佳，高雌性及單為結果性穩定，主要病害露菌病、白粉病、病毒病等抗性基因導入等，育成適合設施栽培之F1品種。本年度進行高雌性雜交後裔F2~F3世代選育，並完成20個東南亞品系供選育自交之材料。另育成13個雜交組合進行品系比較試驗，經果品評估選拔出6個具高雌性及單為結果性之雜交新品系，將於下年度繼續評估。苦瓜為全球重要的葫蘆科蔬菜，在臺灣亦為常見的夏季果菜，主要產區為臺灣中南部地區，本場因地處苦瓜重要產區，為改善苦瓜果實品質與產量，增進農民收益積極進行苦瓜品種改良與栽培技術改進。本場於今年度完成130個苦瓜品系的性狀調查與自交純化，並參考苦瓜品系純化過程的生育特性與果實表現進行評估選拔。針對國內消費市場偏好以選出較優的自交系進行試交， 取得3 0 個試交組(101H01~101H30，其中包含100年評估之優良組合)，於本年度定植評估，其中以101H05、101H06、101H16表現最佳。進行引種收集到4個南瓜商業品種，完成試種栽培性狀調查及自交留種。101冬－102早春栽培西洋南瓜201個自交系、102年春作栽培西洋、美國及中國南瓜157個自交系，秋作栽培121個西洋南瓜等F2-F8世代之品種及單果種子品系，進行性狀調查及自交純化。經評估選出西洋南瓜性狀較佳的56個品系繼續篩選後裔，增進世代。春作並選擇優良自交系進行試交組合24個品系，以及繁殖3個已評估為優良試交品系之種子。秋作栽培品系陸續進行栽培比較與性狀評估，選優留種於下一季種植增進世代。茄子育種目標以提高茄子產量和品質為主，期望選出豐產、抗病蟲、果型長直、品質優良且耐熱性強之品種。本年度將茄子12個自交系，其果實性狀為平均果長27～35公分，果徑25～38mm，果肉白色及淡綠色，果型介於中短胖至中長型，因品系尚未純化完全，持續進行單株選拔，挑選具優良性狀之品系純化。同時蒐集10個茄子新品種進行雜交後裔選拔，進行品種特性調查及雜交後裔選拔。本年度番椒春作進行15個品系、秋作進行48個品系之純化與性狀調查、選拔、留種。春作完成進行11個低辣辣椒新雜交組合進行比較試驗，篩選出102hp5、102hp6、102hp8、102hp12及102hp13等5個組合較佳，秋作完成新雜交組合及其親本種子之量產。彩色甜椒品系11077022-2、11077022-2×sp21及11077022-2×7510於採種期遇升溫時，以10 ppm GA3噴施父母本植株兩次後進行母本或雜交種子生產，提升優良種子數達16.5%以上。番茄雜交組合‘100ht23’父本於25/20℃下，以開花當日之花粉活力最佳，採種品質亦佳，而在較不適合之開花期溫度下，以液態氮保存後之花粉，其發芽率及授粉後之種子量與品質，均有優於新鮮花粉之趨勢。 The main purpose of this project is to breed for promising varieties with desirable characteristic such as high yield superior quality heat tolerance and diseases resistance and to do the seed production tests for the potential new hybrids of cucumber pumpkin bitter gourd pepper and eggplant.The object of this breeding plan is to improve quality of cucumber. We will breed good quality F1 hybrid variety suit for cultivation in subtropical area. The method of breeding is to introduce gynoecious gene into monoecious cultivars and select plants resistant to downy mildew，powdery mildew and virus diseases. We evaluated and selected 13 lines for test crosses for breeding in the spring and combination ability. And through the mouth-feel evaluation we selected 6 lines for test crosses for breeding in the fall and combination ability in next year. This year， we also bred 20 inbred lines and investigated 29 lines of Southeast Asia at the same time， asthe basisoftheimproved varieties.Bitter gourd are important vegetables in Taiwan. Taiwan Seed Improvement and Propagation Station （TSIPS） has bitter gourd inbred lines with good quality and high yield. For the collection and evaluation of germplasms 130 lines accession of them were used to evaluate the genetic diversity of Bitter gourd. It showed significant difference among the gerplasms which revealed that the breeding program could be performed. We planted 30 test crosses in the farm this year. We compared those test crosses which are three lines(101H05、101H06、101H16) better than other lines.The collected 4 squash local and commercial varieties cultivated and characters complete surveyed and produce inbred Seeds. Winter of 2012 to early spring 2013 cultivation pumpkin 201 inbred lines2013 仙履蘭及熱帶球根花卉品種改良與種苗生產技術開發 Improvement on slipper orchid and tropic bulb flowers breeding and establishment the seedling production 102農科-9.2.2-種-X3 102AS -9.2.2-SS-X3 品種改良保護課 Plant Breeding Section 劉明宗 Ming-Chung Liu 依照仙履蘭育種四大目標進行雜交授粉工作，102年進行仙履蘭雜交授粉11組合，出瓶約300株瓶苗移苗栽培工作，目前後裔植株栽培生育中。從雜交組合中以PA94002雜交組合後裔之不同單株具有Paph. primulinum品種多花特性，亦有單花的後裔出現，且花朵會同時開放或者接續開放，因此可再從這些後裔中，篩選單花或雙花特性之後裔單株，PA95016雜交後裔組合，花期約3-4月間開花，花朵顏色為粉紅色，單梗雙花、上萼辦呈橢圓形、翼辦長舌渾圓、整體花型優美，易於栽培，可做為組合盆之用。球根花卉品種選育方面，已篩選具潛力孤挺花單株2株，皆為重瓣具雙梗，未來用途為盆花及切花；孤挺花香味育種方面，在未發育完成之胚珠已成功利用胚挽救技術，獲得50株雜交後裔單株，未來將持續觀察後續生育情形。在彩色海芋方面，94及95年雜交後裔中已篩選具潛力彩色海芋單株3株，為金黃色、紫色及紫黑色。花朵數2-3朵，其中紫色單株花莖較短，可為盆花，其餘為切花。在宮燈百合無性繁殖與實生苗種球養球初步試驗結果，利用自然子球與種子播種而得之實生種球進行比較試驗，結果在三種重量分級中，均以自然子球較實生苗種球重量較重，但均能成功養球。金花石蒜利用無菌播種或胚培養皆可使萌芽率達100%，利用一般播種方式萌芽率僅達65.2%，且栽培一年後，地上部之葉片數則以無菌播種移出種植所生長之葉片數較多達2.01片，一般播種僅1.02片葉。以此推估可縮短養成開花球之年限。 According to four goals in slipper orchids breeding we have selected superior progenies ‘PA94002’ and ’PA95016’ with multifloral good flower shape and color characteristics. Bulbous flowers in calla lilies breeding we have selected 3 potential progenies with good horticultural characteristics. They can be used as cut flower or pot flower. In amaryllis we have selected 2 potential hybrid progenies of doubleflower.Sandersonia tubers from seedling or natural daughter-tuber were planted in mix soilless material under protec ted net greenhouse which were tested for growing flower tubers. The result is flowering tuber is better which were from natural daughter-tuber. The germination rate of spider lily (Lycoris aurea Herb.) is 100% from aseptic and embryo tissueculture but the germination rate is 65.2% from common sowing. Using embryo tissue culture seedlings will be considerable to shorten flowering tubers grown.2013 花卉研究團隊-春石斛開花株生理指標及花期調節管理體系之建立 Set up nobile-type dendrobium mature index and flowering regulation systerm 102農科-9.2.2-種-X4 102AS -9.2.2-SS-X4 品種改良保護課 Plant Breeding Section 郭孄婷 Lan-Ting Kuo 春石斛為國內具經濟栽培及種苗外銷潛力的新興蘭科作物，本試驗擬利用量化之壯苗指數及營養元素測定進行春石斛蘭成熟株生理指標建立，以三個品種根據尾葉及株高區分四組，調查結果顯示，株高、莖節數、葉數、地上部鮮/乾重、壯苗指數與成熟度無明顯關聯，但是地上部含水量在成熟株有增加趨勢，碳/氮含量因氮含量受許多因素影響，不利作為成熟度指標。生長調節劑處理方面，經處理之植株，目前尚未觀察到開花的情形。施用BA有促進葉數增加、腋芽數增加的效果，噴施GA後，有株高增加、腋芽增加及葉片黃化落葉的情形，以品種128最為顯著，若添加BA200ppm，則落葉情形較輕微，有抵消GA效應的作用。本試驗另參試一生長調節劑TDZ 初步試驗結果顯示，經葉面噴施後，可產生大量的花苞，然而，花苞無法正常開放，濃度越高者，消蕾的情況越嚴重。 Noblie-type Dendrobium is one of the important orchids cultivated in Taiwan. In this project we will measure the seedling index and nutrient elements of dendrobium seedlings. Three dendrobium cultivars divided into four groups according to maturation stage and plant height. The results indicated that plant height node number leaf number shoot fresh/dry weight seedling index and maturity were no obvious correlated but water content was increasing in the mature plants. Nitrogen content was affected by species organs and cultivated conditions hence the carbon/nitrogen ration would not appropriate maturation index. As to PGR treatments2013 植物種苗研究團隊-植物品種檢定流程分析、人員訓練及分子標誌開發 plant seed/seedling team – Analyzing the examining process flow training faculty members and developing molecular marker of plant variety right 102農科-9.2.3-種-X1 102AS -9.2.3-SS-X1 生物技術課 Biotechnology Section 張惠如 Hui-Ju Chang (a) 重要蘭科花卉品種分子標誌技術開發：本年度透過委辦計畫與成功大學生物科學系吳副教授文鑾， 針對蝴蝶蘭（Phalaenopsis spp.）商業品種SSR分子標誌鑑定系統，共同建立一套包含十組SSR分子標誌之試驗方法，並制定標準試驗流程，也選定Dtps. Leopard Prince ‘世芥鑽石F-1138’與P. Zuma’s Pixie ‘台大小可愛’兩個蝴蝶蘭商業品種作為標準試驗流程之參考品種。依據已制定之標準試驗流程，鑑定100個已於台灣取得植物品種權蝴蝶蘭商業品種之基因型並以商業軟體建立DNA資料庫，十組SSR分子標誌可完全鑑別100個蝴蝶蘭商業品種，其PIC (polymorphism information content)值介於0.716 (PHS03)與0.893 (PHS07)之間，平均約0.84。並進行多方實驗室之能力試測試與12個蝴蝶蘭商業品種的盲樣試驗，確認所建立標準試驗流程之結果可再現性與穩定性，顯示此套蝴蝶蘭SSR分子標誌品種鑑定系統可有效率協助鑑別蝴蝶蘭品種與國際間相關技術之應用交流。(b) 檢定人員訓練及植物品種權教育推廣：作物新品種檢定講習會於102年10月30日已辦理訓練講習，上課學員約計60人，本次課程內容有植物品種權申請與審查規範介紹、蘭花、蔬菜及糧食作物性狀開發介紹，另外並加入了蝴蝶蘭及黛粉葉檢定技術實習課程，讓上課的學員實際參與檢定技術的操作，實習課程充實，學員熱烈參與。 (a) To develop the molecular marker technology of important orchidflower varieties:To cooperate with Dr. Wu from NCKU through the commissioned project to establish the standard operation procedure (SOP) of Phalaenopsis variety identification comprising ten SSR markers and two Phalaenopsis varietiesDtps. Leopard Prince ‘Sogo F-1138’ and P. Zuma’s Pixie ‘Taida Little Cutie’ were selected as reference varieties of this identification system. The genotypes of 100 Phalaenopsis varieties granted PBR in Taiwan were analyzed based on the established SOP and all these 100 PBR varieties could be discriminated by the ten SSR markers. The PIC (polymorphism information content) values of the SSR markers ranged from 0.716 (PHS03) to 0.893 (PHS07) in average of 0.84. The multi-test and blind tests on 12 Phalaenopsis varieties were also conducted to confirm the data reproducibility and stability of this Phalaenopsis variety identification system. These results revealed that the Phalaenopsis variety identification system based on SSR markers can effectively discriminate Phalaenopsis varieties and accelerate the cooperation of using this applicable technique with other international organizations.(b)Personnel training and promotion of plant variety rights education:New crop varieties DUS testing workshop has been held on 30 October 2013.There are about 60 students in this training class. The curriculum included that application of plant variety rights standard of application form and developed DUS testing of orchids vegetables and grain crops. In addition the operation trait program of Phalaenopsis and Dieffenbachia’s DUS testing technology which will let participators to know the relative technologies of DUS testing and system of plant variety protection. The outcomes of this program will provide helpfully information of plant variety protection and enhance credibility of molecular testing system.2013 植物種苗研究團隊-植物品種開發及種苗驗證之應用研究-植物種苗認驗證體系建構 Construct the Accreditation system for plant seedlings 102農科-9.2.3-種-X2 102AS -9.2.3-SS-X2 生物技術課 Biotechnology Section 孫永偉 Yung-Wei Sun 1.健康種苗之母本檢測與母本園建構及維護：健康種苗之母本檢測與母本園建構及維護為健康種苗繁殖的基礎，本計畫為落實開發的檢測技術與母本園維護，計畫檢測蒐集保存馬鈴薯、葡萄、草莓以及綠竹等作物母本植株，結果顯示葡萄、草莓以及綠竹皆未特定病原，但馬鈴薯有少數蒐集品種感染單一病毒，且未驗證種薯其帶病風險高，藉由持續的母本園維護、母本檢測及更新，期能支持健康種苗產業化之推動。2.瓜類退綠黃化病毒及草莓潛隱輪斑病毒驗證體系建構：參酌國內外文獻，已完成瓜類退綠黃化病毒（Cucurbit chlorotic yellows virus CCYV）與草莓潛隱輪斑病毒（Strawberry latent ringspot virus SLRSV）之檢測技術盤點。瓜類退綠黃化病毒目前檢測技術主要有： RT-PCR、Western blotting、ELISA及Immunoelectron microscopy；草莓潛隱輪斑病毒目前檢測技術主要有：RT-PCR、IC-RT-PCR、ELISA與病徵判別。以不同的樣品進行各種檢測方法的方法測試，測試結果瓜類退綠黃化病毒以RT-PCR檢測效果較佳；草莓潛隱輪斑病毒則以ELISA檢測效果較佳，挑選最適合的檢測方法建立標準檢測流程，並通過能力試驗。3.十字花科種子健康檢查驗證體系之建立：本計畫目的係為建立種子檢查室之十字花科種子黑腐病菌檢測作業流程。已依國際種子檢查協會規定之檢測方式建立檢測流程。以人工汙染種子進行檢測流程的測試，可於FS及mCS20ABN兩種選擇性培養基上觀察到測試菌落的標準型態，且靈敏度測試結果可達0.01%的帶菌率。經測試7批不同公司生產的十字花科種子樣品，均未測得Xcc。品質管理所需之表單亦已建置完成。4.種子(苗)品質純度分子檢測技術研發及標準化：番椒利用F2族群及ISSR之DNA片段多型性，建立F1種子純度分子檢測識別標誌，以充實番椒雜交一代種子純度分子檢測之分子標誌資料庫。結果番椒13個多型性ISSRDNA片段成功回收12個，經定序、解序共獲得8個序列，重新設計條帶專一性引子，其中引子組合11組經最適PCR反應捻合温度及配方調整，均具條帶專一性。但以建立之番椒F2材料測試結果均未表現多型性，因此其識別性尚待進一步確認。番茄3個品種9個植體材料(雜交一代及父、母本親)，利用ISSR-PCR技術篩選共獲得5個新的識別標誌，其中3組引子表現條帶專一性。經最適PCR反應捻合温度及配方調整，標誌大小分別為385、463及626 bp，電泳圖呈現引子高度的條帶的專一性及識別性。 Virus and pathogen detection and establishment of mother-stock garden is the fundamental of healthy seedling production. In this program we will implement the developing pathogen validation system and construction mother-stock garden to maintain specific pathogen free mother-stock such as potato grapes strawberry and bamboo etc. Our results indicated grapes strawberry and bamboo kept in mother-stock garden were not infected by specific pathogens. Potato stocks were infected by potato virus S or potato virus Y and using none certification potato seed tube would face higher infection risk. We hope this effort could support healthy seedling production in Taiwan.To refer to the scientific or technical literature we completed the detection technology inventory of Cucurbit chlorotic yellows virus CCYV and Strawberry latent ringspot virus SLRSV. The main detection technologies of CCYV are RT-PCRWestern blotting2013 植物種苗研究團隊-種子品質及處理技術研發 Developement for the seed processing techniques to promoting seed quality 102農科-9.2.3-種-X3 102AS -9.2.3-SS-X3 種苗經營課 Seed & Seedling Management Section 黃玉梅 Yu-Mei Huang 不公開 02013 植物種苗研究團隊-植物新品種檢定技術之開發與執行 Plant Seed Group-The Technical Development and DUS Test Execution of Plant Variety 102農科-9.2.3-種-X4 102AS -9.2.3-SS-X4 品種改良保護課 Plant Breeding Section 安志豪 Chih-Hao An 本年度已完成訂定藍眼菊與瓢唇蘭亞族並修訂蝴蝶蘭與茼蒿品種性狀表與試驗檢定方法及草擬合果芋品種性狀表初稿，透過增修訂品種性狀表及試驗檢定方法，擴增國內植物品種權受保護之植物種類。關於蝴蝶蘭、文心蘭及番茄等作物資料庫建立方面，已收集15個蝴蝶蘭、5個文心蘭及番茄商業品種並完成品種性狀調查及資料庫建置，透過資料庫建置，以利未來對照品種之資料比對搜尋。本年度受理新品種性狀檢定工作計蝴蝶蘭與朵麗蝶蘭99件、文心蘭6件及玫瑰6件；正進行性狀檢定中之案件為蝴蝶蘭與朵麗蝶蘭21件、文心蘭8件及玫瑰8件；檢定完成資料整理中為蝴蝶蘭與朵麗蝶蘭12件；檢定完成且審查結束為蝴蝶蘭與朵麗蝶蘭47件及玫瑰2件，透過植物品種權制度，確保植物育種者權利及品種保護之效力，同時也提升農業產值之提升。 The purpose of this project is to establish the test guidelines and execute DUS tests. We modified the test guideline and table of characteristics of osteospermum araceae catasetinae and chrysanthemum.For Cooperation of phalaenopsis plant variety protection technology between Taiwan and European we revise the test guideline and table of characteristics of phalaenopsis. We also collected 15 commercial varieties of phalaenopsis and doritaenopsis and 10 commercial varieties of oncidium and rose and investigated their characteristics for establishing variety database. We accepted 99 cases of phalaenopsis and doritaenopsis 6 cases of oncidium 6 case of rose to execute DUS tests for protecting the breeder’s rights this year. 21 cases of phalaenopsis and doritaenopsis 8 cases of oncidium 8 cases of rose have been executing DUS tests. 47 phalaenopsis and doritaenopsis and 2 roses have been granted to be new varieties2013 植物種苗量產體系建立 Establishment of mass production system of plant seedlings 102農科-9.2.3-種-X5 102AS -9.2.3-SS-X5 繁殖技術課 Propagation Technology Section 王至正 Jhih-Jheng Wang 不公開 Not public2013 植物種苗研究團隊-亞太地區植物種苗產業研究與應用 Plant nursery research group-Studying and application on plant seed industry in Asia-Pacicic region 102農科-9.2.3-種-X6 102AS -9.2.3-SS-X6 技術服務室 Technical Service Section 周明燕 Ming-Yenn Chou 不公開 Not public2013 作物遺傳種原收集、保存與利用 Preservation collection and application of germplasm 102農科-9.2.3-種-X7 102AS -9.2.3-SS-X7 品種改良保護課 Plant Breeding Section 薛佑光 Yu-Kuang Hsueh 1.蔬菜種原收集、保存及利用更新蔬菜種原庫完成63個品種之栽培、調查及種子繁殖採收，調製包裝貯藏於種原庫。其中包括新收集的30 個萵苣品種種原繁殖，並進行萵苣之硝酸鹽累積之檢測，結果顯示品種間對硝酸鹽的累積具顯著差異，比對歐盟的規定(種植在遮蓋下)，僅024、026、027及038合乎規定，大部份都超過容許範圍，推測應與田間肥力有關。2.臺農種苗2號梨母本園管理與保存梨臺農種苗二號 (蜜雪梨)種原於101年12月底進行樹幹深剪枝，建立優良樹型。陸續進行灑施有機質肥料，病蟲害防治，主要防治項目為介殼蟲、赤星病、黑星病、梨綠蚜、黑斑病與白粉病。為使樹體健壯，於3月20日再施放43號肥料，每株20公斤。4月底陸續開始疏果作業、套袋作業開始， 7月16日起開始進行採收作業。蜜雪梨收穫量為2314.5公斤，果品糖度平均為10.12Brix，較歷年來偏低，推測原因為今年前半年數個颱風、豪雨及採收前颱風及豪大雨所致造成蜜雪梨果品糖度及品質不如歷年來的好。3.本土綠化種苗種原收集及保存102年已收集本省原生綠化苗木，計台灣樹蘭、台灣山桂花、黃皮、白水木、鐵色、長葉紫珠、霧社櫻、楊梅、杜英、五掌楠、朱砂根、台東火刺木、穗花棋盤腳、椬梧、蘭嶼羅漢松、台灣油杉、珊瑚樹、台灣扁柏、石斑木及淡綠葉衛矛等20種。除了朱砂根因發育緩慢及鐵色生育不佳重新扦插處理(目前還在24格穴盤中持續生育)，其餘18種原生綠化苗木都已移植入6吋盆中。此20種原生綠化苗木為楝科、芸香科、楊梅科、樟科、紫草科、大戟科、馬鞭草科、杜英科、玉蕊科、胡頹子科、羅漢松科、松科、忍冬科、柏木科、衛矛科、2種紫金牛科及3種薔薇科。4.綠美化苗木種原保存蒐集－－殼斗科全年收集13種殼斗科種原，分別為青剛櫟、小西氏石櫟、槲樹、短柄枹櫟、後大埔石櫟、鬼櫟、阿里山三斗石櫟、大葉石櫟、捲斗櫟、高山櫟、狹葉櫟、太魯閣櫟、栓皮櫟。植株生育健壯後，再調查生長狀況及性狀等以待種原保存用。以青剛櫟為試驗材料，利用兩種栽培介質及四種育苗容器作為處理，調查每階段青剛櫟種苗之生育情形，總體表現看來兩種育苗介質處理(有機介質、田土與有機介質混合比例1:1)之間無明顯差異，育苗容器處理上，目前以6吋硬塑膠盆、6吋黑寬軟盆植株表現較好，將繼續觀察各處理苗株生育表現，期能選出最適景觀綠化容器苗管理模式。5.台灣香藥草植物資源開發利用台灣本土多樣性資源豊富，利用既有的農業科技基礎，保存植物種原，經濟栽培台灣野生植物及開發利用植物種子種苗有利於生態保育。本計畫建立及保存香藥草資源650種並進行9種香藥草植物抗氧化分析，以月季及大風草之抗氧化能力最佳。 1. Renewed vegetable germplasm for 63 varieties of cultivated investigation harvesting packaging and stored seed. Including 30 new lettuce varieties collected and nitrate accumulation for testing the results showed that nitrate accumulation between varieties with significant differences compared to the EU regulations (grown under cover) only 024 026027 and 038 compliance most of them are over the allowable range2013 有機團隊-蔬菜有機種衣劑及種子有機處理基準之研究 Establishment of vegetables organic coating material and organic processing benchmark in seedling 102農科-9.2.4-種-X1 102AS -9.2.4-SS-X1 種苗經營課 Seed & Seedling Management Section 洪建民 Chien-Min Hung 不公開 Not public2013 有機團隊-番木瓜有機栽培及有機雜交種子生產研究 Study on organic cultivation and organic papaya seed production 102農科-9.2.4-種-X2 102AS -9.2.4-SS-X2 屏東種苗中心 Pingtung Seed & Seeding Research Center 邱展台 Chan-Tai Chiu 本試驗擬於春夏季定植木瓜，以植株幼年期度過雨季避開疫病危害，並於冬春季果實炭疽病發生率較低時開始成熟採收。另以礦物油及煙草抽出液防治螨類及秀粉介殼蟲危害，並調查以有機肥料取代化學肥料的效果。試驗結果顯示煙草抽出液無法抑制螨類，窄域油於螨類發生初期可抑制滿類族群，使用2-3次後，即失去效果，只能作玉預防性噴灑。雨季木瓜成熟果實病害發生嚴重，窄域油無法克服只能以避病方式克服。 Papaya were planted on spring and summer respectinely childhood spent the rainy season to plant to avoid disease hazards and to begin to mature at lower incidence of anthracnose of fruits in winter and spring harvest. Extraction of mineral oil and tobacco extraction control of mites and scale insects results of the investigation to be replaced by organic fertilizers and chemical fertilizers. Test results exhibited that tobacco extract could not inhibit mites; narrow range oil can inhibit the full class in the early colony after 2-3times which no longer worksbut jade preventive spraying. Rainy season mature fruit papaya disease seriously narrow range oil failed to overcome the disease can only be overcome by avoid disease.2013 作物種苗病害防治技術之開發與應用 Development and Application of Crop Seedling Diseases Controlling Technologies 102農科-10.2.5-種-X1 102AS -10.2.5-SS-X1 繁殖技術課 Propagation Technology Section 袁雅芬 Ya-fen Yuan 本年度已取得27個南瓜品系共324個樣本進行白粉病原菌接種試驗；同時自不同地點收集白粉病原菌進行繁殖與保存；並建立南瓜對白粉病抗性之病害等級；本年度接種的品系皆為較感白粉病特性之品種。經16S rDNA 序列分析結果，鑑定101年度篩選出編號14-12與15-25的拮抗微生物均為巨大芽孢桿菌(Bacillus megaterium)；測試11種培養基組合均未能使編號14-12與15-25拮抗微生物的細胞濃度達109/ml，但是由試驗結果可知添加適當鈣鹽有助於細胞生長。無性繁殖體在進行組織培養大量繁殖前，進行標的病害的檢測可確保繁殖體的健康，為生產健康種苗之基石。組織培養的標的病害包括病毒病、類病毒病以及菌質體病害，多以enzyme-linked immunosorbent assay (ELISA)、PCR(reverse transcription-PCR RT-PCR)，作為檢測植物病毒病之技術，其中ELISA仍是主要檢測植物病毒病之技術，血清為此技術之重要成本，因此本計畫擬以大腸桿菌表現轉殖之重組蛋白，作為生產標的病毒之蛋白來源，生產重要作物之檢測血清，本計畫於完成產馬鈴薯病毒PVS、PVX、PVY之抗血清之生產各80ml，力價稀釋可達8000-16000倍。 Twenty-seven different pumpkin hybrid lines were screened to the powdery mildew -resistance which inoculum pathogen species. According the inoculum results showed that the virulence were different by the host hybrid lines. The hybrid lines were almost easy to infect by the powdery mildew in these year. We construct a grade of the powdery mildew –resistance in pumpkin.By 16S rDNA sequences analysis antagonists No. 14-12 and 15-25 which were selected in 2012 were identified as Bacillus megaterium. We have tested 11 media formulas yet non of them had the effect to make the cell concentration of antagonists No. 14-12 and 15-25 up to 109/ml. Howeveraccording to the results it was found that adding suitable calcium salt could promote cell growth.Before enter a large number of plant tissue culture propagation to detect the plant target disease can ensure a healthy production procedure. The specific pathogen free tissue culture units also are the cornerstone for the production of healthy seedlings. Tissue culture is the subject diseases including viral diseases viral diseases and class mycoplasma disease. And techniques for detect these pathogen are enzymelinked immuno-sorbent assay ELISA and Reverse transcription-PCR RT-PCR.The cost of serum is the highest value of ELISA. This project is intended to express recombinant proteins in transgenic E. coli2013 植物種苗研究團隊-種子(苗)有害生物診斷鑑定技術、檢驗技術驗證及防治技術之研究與應用 Establishment and Research of Seed and Seedling Certification Techniques and Regulations 102農科-10.2.5-種-X2 102AS -10.2.5-SS-X2 繁殖技術課 Propagation Technology Section 邱燕欣 Yen-Hsin Chiu 不公開 Not public2013 耐溼冷馬鈴薯品種選育 Wet and cold resistant breeding for potato 102農科-14.1.2-種-X1 102AS -14.1.2-SS-X1 品種改良保護課 Plant Breeding Section 張勝智 Sheng-Chih Chang 因近年來全球氣候異常，造成溫度及雨量變化劇烈。台灣冬季為馬鈴薯栽培期，因異常的低溫高濕氣候，導致植株容易腐敗及病害發生嚴重進而減產，嚴重影響農民收益並造成生產成本增加。因此，本場利用所選育具優良生育特性之48個營養系，配合本地冬季低溫環境，進行低溫高濕的汰選評估，經100/101年期的汰選，於101/102年期進行11個具耐濕與高產生育特性的營養系(628、474、470、91A179、320、183、578、91N80、317、T75、462)與對照品種克尼伯進行耐濕冷特性、加工儲藏評估及晚疫病接種評估，完成選出5個分別(營養系470、628、91N80、320、462)具耐濕冷、生長勢強、高產、高耐病性(耐晚疫病)等特性之營養系。期許能協助農民或種苗業者克服因不良環境造成的減產問題以及農藥大量施用，所造成食品安全的問題。此外，亦可選育不同品種作為調適氣候變遷的影響，維持國內馬鈴薯生產的穩定。 The global climate change intensive recently. In Taiwan intensive heavy rains frequently during the winter resulting soil wet and sticky and thus bring down the quality of potato and decrease the yield. So the wet tolerance variety of potato is a greater demand. Screening out the wet tolerance strain of potato for producer to enhance the competitiveness of potato industry in the future.In this projectwe will select 11 clones (628、474、470、91A179、320、183、578、91N80、317、T75、462) with Kennebec (CK) in winter for field cultivation to assess wet stress resistance and disease susceptibility .We investigate the potato characteristics quality yield and potato storage of survey. We select 5 clones (470、628、91N80、320、462) which have good characters. We hopethat we can assist farmers and seed industries overcoming the reduction of output and problem of food safety. In addition we select different varieties to be adapt the weather and maintain the potato production in Taiwan.2013 設施內茄科蔬菜利用蜜蜂授粉生產之研究 Study on honeybee pollination for Solanaceae vegetable production in the greenhouse 102農科-14.1.2-種-X2 102AS -14.1.2-SS-X2 品種改良保護課 Plant Breeding Section 郭宏遠 Horng-Yeuan Kuo 利用蜜蜂於網室內高溫期進行彩色甜椒果實生產時，於植株第一朵開花前一星期將蜂箱放入網室內使蜂群馴化適應。網室內之光度及溫濕度影響蜜蜂離巢及訪花之頻率，以晴天高光度及非中午高溫期之時段離巢訪花頻率高。遮陰處理配合蜜蜂授粉之果形、果肉厚度、單果重及糖度均優於遮陰處理下自然授粉者。 Honeybee hive should be put into the net-house one week before anthesis of first flower for honeybee acclimation when sweet pepper fruit production in the net-house during high temperature period. Lighttemperature and relative humidity in the net-house would affect the honeybee activities of leaving hive and foraging the frequency of leaving hive and foraging was high when in high light intensity and nonhigh temperature period on sunny days. In addition the fruit shapeflesh thickness fruit weight and sweetness of fruit were better in honey bee pollination with shading treatment than those of self-pollinated with shading.2013 應用綠肥營造環境親和型水旱田輪作經營模式 The establishment of environmental friendly crop rotation model usinggreen manure 102農科-14.1.3-種-X1 102AS -14.1.3-SS-X1 種苗經營課 Seed & Seedling Management Section 鄭梨櫻 Lee-Ying Cheng 為因應氣候變遷對台灣雜糧生產之考驗及現行耕作制度的衝擊，以飼料玉米品種選擇搭配環境親和型栽培方式，規劃2年1輪環境親和型水旱田輪作模式，規劃重點為輪作綠肥與水旱田輪作。試驗結果，輪作綠肥相較於對照組可減施化學肥料避免土壤酸化、提升土壤有效性磷含量及降低土壤電導度，減緩土壤鹽化。以土壤地力維護及輪作收益為評估指標，輪作模式B為較佳之輪作模式。 In response to the impact of climate change on the grains production and existing farming systems in Taiwan applied different feed corn varieties with environment-friendly cultivation methods planned for 2-years a round crop rotation patterns.The rotation patterns focuses on paddy field and dry land rotation and green manure application. The results showed that compared with the control green manure application can reduce chemical fertilizer usage to avoid acidification improve soil phosphorus contentreduce soil EC value and slow down soil salinization.Soil fertility maintain and cash earnings of crop rotation for theassessment index rotation mode B is the preferred mode of rotation.2012 植物種苗研究團隊-基因轉殖及非基因轉殖種苗驗證及共存體系之建構-基因轉殖作物認驗證與檢監測模式之建立 Construction of the certification and coexistence system for GM and non-GM seedlings 101農科-1.1.1-種-X1 101AS -1.1.1-SS-X1 生物技術課 Biotechnology Section 沈翰祖 Han-Tsu Shen 基因轉殖作物檢監測體系由農糧署委託種苗改良繁殖場邀集農業試驗所、桃園區農業改良場、台南區農業改良場、花蓮區農業改良場、國立中興大學等單位建立「基因轉殖作物檢監測小組」，藉由「檢測技術建立」、「儀器與檢測精準度試驗」、「檢測樣品檢出能力試驗」、「能力試驗」等，已建立基因轉殖木瓜2個、玉米10個、大豆3個以及馬鈴薯1個品項之標準化檢測模式，其中大豆及馬鈴薯已向「財團法人全國認證基金會（Taiwan Accreditation Foundation TAF）」之實驗室提出增項認證申請。本年度檢監測小組進行基因轉殖大豆3次、玉米2次能力試驗。核酸定量檢測之量測不確定度(measurement uncertainty MU) 以bottom-up與topdown等兩種評估策略建立評估模式，以歐盟建議之試驗條件所得不確定度數值為30.6%，依儀器所提供條件所得不確定度數值為29.81%。已完成基因轉殖玉米與傳統農業共存栽培建議書與基因轉殖作物標準化取樣及監測模式，並以田間試驗模擬基因轉殖大豆花粉飄散趨勢，本年度春作試驗並未發生花粉污染之情形。已利用衛星定位(GPS)監測台灣水稻28個、油菜10個、馬鈴薯4個、玉米10個、大豆11個栽培區、野生大豆9個生長區等資料，且以地理資訊系統(Geographic Information System GIS)分析是否受到基因轉殖作物污染，並完成可攜式GIS系統。 The detection and monitoring system of transgenic crops in Taiwan was entrusted by the Agriculture and Food Agency (AFA) Council of Agriculture (COA) and the team member is Agricultural Research InstituteTaoyuan District Agricultural Research and Extension Station Tainan District Agricultural Research and Extension Station Hualien District Agricultural Research and Extension Station National Chung Hsing University and Seed Improvement and Propagation Station (SIPS). Technique for detection of GM crops consists of four distinct steps including detection capability proficiency test2011 木瓜全兩性株基因體定序研究(委辦) Study on the whole genome sequence of all hermaphorodite papaya cultivar 100農科-1.1.8-種-X4 100AS-1.1.8-SS-X4 生物技術課(中興大學) Biotechnology Section(National Chung Hsing University) 張惠如(劉俊吉) Hui-Ju Chang(Chun-Chi Liu) 不公開 Not public2010 作物種苗病害檢測、驗證及防治技術之開發與應用 Application and development of detection certification and control of crop diseases 99農科-9.2.1-種-X1 99AS-9.2.1-SS-X1 繁殖技術課 Propagation Technology Section 鍾文全 Wen-Chuan Chung 不公開 Not public2012 植物種苗團隊-基因轉殖作物種苗檢測監測體系之建立 The establishment of detection and monitors system for genetically modified seedling 101農科-1.1.1-種-X2 101AS -1.1.1-SS-X2 生物技術課 Biotechnology Section 張珈錡 Jia-Ci Chang 持續執行基因轉殖作物種苗TAF檢測實驗室，於本年度進行一次監督評鑑、一次認證實驗室內部稽核與一次認證實驗室年度管理審查會議。另完成實驗室電腦條碼管理系統套裝化測試，與配合農糧署辦理二場基因轉殖作物檢測討論會。並利用Realtime PCR建立基因轉殖玉米TC1507、BT-176、Bt11、MON810、NK603、3272、MIR604、GA21、59122、MON863等品項、基因轉殖大豆GTS 40-3-2、DP-305423-1、DP-356043-5等品項之螢光快速檢測技術，R2均達0.9以上。 The increasing presence of transgenic plant in the international markets has provoked a strong demand for appropriate detection methods to evaluate the existence of transgenic plants. Technique for detection of GM crops consists of four distinct steps including detection capabilityproficiency test the standard detection and monitoring model. The biological area of detection laboratory at SIPS has been accredited according to ISO/IEC 17025. There are several items to be accredited by TAF including transgenic papaya (Papaya ring spot virus coat protein gene prsv-cp gene; Papaya ring spot virus coat protein gene PRSV CP gene；papaya leaf distortion mosaic virus PLDMV～py16-cp gene) maize (TC1507 BT-176 BT112012 植物種苗團隊-植物種苗產業發展服務平台 Development and Service Platform forPlant Seed/Seedlings Industry 101農科-1.1.2-種-X1 101AS -1.1.2-SS-X1 繁殖技術課 Propagation Technology Section 李美娟 Mei-Jiuan Lee 不公開 Not public2012 植物種苗團隊-番木瓜種苗七號全兩性株調控基因分析與產業應用 Analysis of regulation genes of allhermaphrodite papaya cv. TSS No.7 and its application on industry 101農科-1.1.2-種-X2 101AS -1.1.2-SS-X2 繁殖技術課 Propagation Technology Section 李美娟 Mei-Jiuan Lee 不公開 Not public2012 種子檢查技術研習 Study on seed testing technology 101農科-4.2.1-種-X1 101AS -4.2.1-SS-X1 種苗經營課 Seed & Seedling Management Section 許鐈云 Ciao-Yun Syu 今年度本場派員1名參加國際種子檢查協會(International Seed Testing Association ISTA)認證實驗室(Accredited Laboratory)舉辦之種子活力檢查(Seed Vigour Testing)及種子發芽率檢查(Seed Germination Testing)研習課程，出國期間為101年9月8-21日。其中，種子活力檢查研習係20/20種子檢查室(20/20 Seed Labs Inc.)於愛特蒙頓舉辦；種子發芽率研習係加拿大食品檢驗局(Canadian Food Inspection Agency CFIA)於薩斯卡通舉辦，本研習計有加拿大、美國、英國 、德國、法國、荷蘭、澳大利亞、日本及我國等國家種子檢查人員參加。此次研習內容主要包含ISTA及種子檢查規則簡介、種子活力檢查方法(電導度法、胚根觀察法、人工老化法)、種子發芽率檢查方法(單子葉及雙子葉作物種子實習)、品質保證、ISTA檢驗證填寫及參觀加拿大食品檢驗局(Canadian Food Inspection Agency)種子實驗室及國家種子標本館(National Seed Herbarium)，並於課後請CFIA潔淨度人員講授禾穀類作物、豆科作物及十字花科作物種子潔淨度分析操作流程。 In this year one researcher of TSS attended two ISTA workshops on 8-21 Sept.2012 in Canada . The Seed Vigour Testing workshop was hold by 20/20 Seed Labs(Accredited Code: CA10) in Edmonton and the Seed Germination Testing workshop was hold by Canadian Food Inspection Agency (CFIA) (Accredited Code: CA08) in Saskatoon. The participators in these ISTA workshops came from Canada U.S.A. U.K. Germany France Netherlands2012 赴荷蘭研習健康種苗產程管理系統中產品品質與病理管控點之設立 Establishment of verification techniques and critical control points for healthy seedling management system from Netherlands 101農科-4.2.1-種-X2 101AS -4.2.1-SS-X2 繁殖技術課 Propagation Technology Section 袁雅芬 Ya-fen Yuan 台灣現階段驗證檢查架構以生產期間之控管為主。除了在行政架構上兩國極明顯不同，荷蘭在馬鈴薯種薯生產策略與產程管理上與我國現行系統不同，尤其在於收成後庫存管理、批次管理與認證追蹤上皆有技術及系統建立，相較目前於台灣並無此管理系統，實值得向荷蘭借鏡，作實地參訪與學習。與荷蘭相關單位接洽，完成行程安排與規劃重要行程: 1. 建立雙方在健康種苗驗證制度上技術經驗及資訊上交流之管道。2.提升我國在健康種苗產程管理執行之技術。 The health seedling supply system is the most basement of agriculture production. Taiwan small but densely populated has excellent location as an International Transportation Center in Southeast Asia. Our government needs to plan the trend and development for agriculture to focus at soft power including excellent traditional breeders and high biotech genetic-engine researchers as backing to develop the seeding industry and make the seed industry refined and internationalization in Taiwan. The future goal is to make Taiwan as the Asia-Pacific Plant Seedling Centre which constructed for the export market-oriented. To construct the healthy seedling procedure management system is one of the nucleus strategies to make Taiwan as the Asia-Pacific Plant Seedling Centre.TSS starts to construct the whole R ＆ D production procedure of health seedling healthy seed production system process control and industrial applications.And TSS also make connection and meet the demands of the international validation and certification system.The Netherlands is internationally renowned for its practical approaches and applications in the seedling industry since the first bulb test laboratory was set up. Cooperation between public (Plant Protection Service PD) and private sectors (including: Netherlands Inspection Service for Horticulture；Naktuinbouw NAKT; Flower Bulb Inspection Service BKD; Dutch General Inspection Service for Agricultural Seed) has resulted in innovative developments in breeding and variety developmentseed production storage seed certification and marketing. The Dutch approach to seed sector development and worldwide partnerships provide new insights and knowledge for the sustainable development of seedling supply systems in developing countries or countries with an economy in transition.In Taiwan2012 赴荷蘭研習利用分子標誌進行花卉及蔬菜品種鑑(檢)定 The study on molecular marker and DUS test techniques used for plant variety protection in Netherlands 101農科-4.2.1-種-X3 101AS -4.2.1-SS-X3 生物技術課 Biotechnology Section 張惠如 Hui-Ju Chang Naktuinbouw為荷蘭執行植物品種檢定的專責機構，亦負責歐盟成員國數種作物品種申請案件之檢定工作，具相當豐富的品種檢定業務經驗及人力。本年度於8月19日至9月1日前往Naktuinbouw進行研習，研習內容主要分為：一、蝴蝶蘭、其他蘭花(萬代蘭、文心蘭)、飄香藤等重要經濟作物之品種檢定，透過實質檢定、雙方檢定技術交流、性狀調查表開發及結果討論；二、蝴蝶蘭SSR分子標誌分析方法比較與討論、研擬未來雙方可行之合作方式與DNA資料庫的建立方法等。透過與其技術人員的交流與經驗分享，可在往後操作上更加留意一些可以增加準確度的地方，進而調和雙方技術與檢定結果。除前述技術之實務操作與討論，建立雙方溝通及交流管道外。並蒐集有關Naktuinbouw之品質監管及作業程序等資料訊息，期有助於促進國內檢定技術的提升及經驗之累積。 Naktuinbouw is the only authorized organization in the Netherlands which is responsible for DUS – testing of all varieties of ornamentalvegetable and agricultural crops. And Naktuinbouw is not only responsible for domestic applications but also for applications in EU. The study was mainly done in Naktuinbouw from Aug. 19th to Sep. 1. This study included doing the descriptions learning how to develop a test guideline and revise it by discussions and operations. The learning was especially involved in Phalaenopsis other orchids and other ornamentals. And the study also included visiting the Laboratories to use the same SSR primers and DNA of Phalaenopsis cultivars registered in Taiwan to do experimental analysis and then discuss some questions based on the experimental results. To enhance the standardization and authority of the technologies which were established through cooperation and technical exchange of both sides.2012 植物種苗農業職能及訓練管理成效之研究 The study of competency standard and training management in plant seedling industry 101農科-5.2.2-種-X1 101AS -5.2.2-SS-X1 技術服務室 Technical Service Section 鍾依萍 I-Ping Chung 教育訓練的目的是要增強農民的能力、提升農民解決問題之技能，使達到降低生產成本，以及提高作物品質之目標，除積極生產及管理技術之訓練外，對於訓練之成效以及推廣服務品質並應持續給予評估與改進。本計畫在「種苗生產暨管理技術輔導訓練」辦理種苗技術訓練班10梯次（入門4梯次、初階1梯次、進階4梯次、高階1梯次）、種苗專題講座6場、發行種苗科技專訊4期（7200本）。在「在訓學員訓練發展及成效評估」針對入門4梯次、初階1梯次、進階-組培班3梯次進行訓練考評調查，學員在反應層次評估，各項評估非常好及好者皆有85.70%以上；在學習層次評估方面進行進階班學員受訓前與後之測驗，得分由58.68分進步至78.51分進步程度達134%，顯示訓練後有能力改變之情形。問卷分析得知，各階段學員來受訓的目的不同；未來投入或繼續從事農業工作的意願方面，願意者皆有9成以上，想投入或想繼續從事農業工作的原因，皆是以「個人興趣」最高、其次為「家中有農地」或「看好農業前景」，顯示學員對農業有高度的興趣並看好其前景與發展。在「結訓學員追蹤調查」部份，是以電訪方式調查98~101年參加本場進階訓練班－組織培養訓練、穴盤育苗課程之結訓學員從農情形，有從農者佔79.55%、兼農者佔18.18%、未從農者佔2.27%；從農的年齡分佈集中於41-60歲（58.33%）。建議未來在課程規劃上能多吸引青年人加入，並持續提供現有從農學員所需課程與輔導，調查與關注學員從農面臨的問題，讓有意從農與仍在從農的學員能更積極地投入農業。 The aim of educational training is to enhance the farmers’ abilities in problem solving. In addition to the energetic production and the training of management skill the training results and service qualities should be evaluated and improved continuously. This project conducted 10 training courses of seeding skill and divided 4 different levels courses which included 4 periods of entry 1 period of beginners 4 periods of advanced and 1 period of high-level. Also it conducted 6 seeding subject workshops and published seasonal seeding journals which issued 7200 volumes in total.According to evaluation questionnaires of the training courses in the level of entry beginners and advanced more than 87% of the trainees have shown both great and good in reaction. Moreover the evaluation of learning in advanced shown that the test results from before to after training has progress 134%2012 建構亞太植物種苗產業服務資訊平台 Construction for Asia Pacific Seedlings Industry Services Platform 101農科-7.2.1-種-X1 101AS -7.2.1-SS-X1 技術服務室 Technical Service Section 蔡瑜卿 Yu-Ching Tsai 一、 亞太植物種苗產業服務資訊平台之建置完成亞太植物種苗產業服務資訊平台之建置，進行系統安裝及測試、相關文件編撰，本場既有10個資料庫進行資料整理共轉錄5016筆、新增資料452313筆至新建置的亞太植物種苗產業服務資訊平台，並完成2場教育訓練課程、本服務資訊平台營運模式規劃報告、植物種苗產業之育種領域技術計量分析報告各一份。二、蝴蝶蘭品種影像辨識輔助系統開發建置自動化的蝴蝶蘭品種檢定影像辨識系統，首先必須對蝴蝶蘭花朵的上萼瓣、下萼瓣、翼辦及唇瓣做影像的色彩、形狀、紋理特徵擷取。利用這些特徵向量查詢比對資料庫中每一個既有的蝴蝶蘭資料與待檢定之蝴蝶蘭的相似度，如此一來，鑑定人員可以輸入想要檢定的蝴蝶蘭品種之影像，系統會將所輸入之蝴蝶蘭影像與資料庫中已登錄的蝴蝶蘭品種進行比對，然後輸出排名前幾名的結果相似的品種(由高分到低分)回傳給檢定人員參考，以此來協助檢定人員判斷此蝴蝶蘭影像是否為新品種。 "1.A study of Constructing Asian Pacific Seedlings Industrial Information　Services Platform　Since the technology of information and communication and seedling　industry are developing rapidly the agricultural public sector must be　able to provide further depth and breadth of real-time industrial　information services for seedling industry towards highly refined and　international development. However information scattered repetitive or　uneven quality problems will be encountered while the amount of　information is continuous increasing. Therefore to plan and constructs　an integrated industrial information services which providing diversified　industrial information services to satisfy the information needs of the　seedling industry should be an important research issue. The research　used the methods of content analysis interviews questionnaires and　expert panels; it firstly investigated 10 database resources and nine　related domestic industrial service information platforms to study the　current status of industrial information resources and services. It　followed conducted a questionnaire survey and interviews to understand　the requirements of information services from users of seedling industry　so as to plan and construct the industrial information services platform.　The general construction of the Asian Pacific plant seedling industrial　information services platform contains platform purpose contents and　functions. One of the functions of the ""Industrial Services"" covers a　variety of information technical services2012 花卉團隊-以化學性誘變法進行馬拉巴栗新品系選育 Breeding of Pachira macrocarpa via chemical mutagenesis 101農科-9.2.2-種-X4 101AS -9.2.2-SS-X4 品種改良保護課 Plant Breeding Section 郭孄婷 Lan-Ting Kuo 將採收之馬拉巴栗果實剖開，取出種子，以NaN3不同濃度進行浸種，分別為0 mM、0.5mM、1mM及2mM，浸種24hr、48hr，計算其發芽率並觀察誘變之結果，0 mM、0.5mM、1mM及2mM NaN3處理24hr，種子之發芽率分別為11.43%、10%、22.86%及11.76%，處理48hr後，種子之發芽率分別為0%、0%、1.52%、12.12%。目前已有三株變異株，變異之性狀分別為葉脈中間具有淺色斑紋及節間短縮，葉形細長捲曲。組織培養建立方面，未成熟種子切段進行癒合組織誘導，可觀察到各段節於傷口處有癒合組織產生，以含24-D 1mg/L之培養基效果較佳，24-D 2mg/L之處理，其培殖體易褐化，部分處理則有子葉轉綠之情形，逆分化效果較輕微。所產生之癒合組織多發生於切口處，呈現鬆散狀，無觀察到擬胚的產生，需進一步調整培養基，若以含24-D及2ip之培養基繼代培養，癒合組織可持續生長。 The seeds of Pachira macrocarpa were collected and treated with 0mM0.5mM 1mM and 2mM for 24hrs or 48hrs. After treatments the hygrochastic seeds were planted apart. Then counted the germination rates of seeds and observed if there was mutation. The germination rate of hygrachastic seeds treated with 0 0.5 1 2mM NaN3 for 24hrs were 1.43%2012 植物種苗團隊-植物品種檢定流程分析、人員訓練及分子標誌開發 plant seed/seedling team – Analyzing the examining process flow training faculty members and developing molecular marker of plant variety right 101農科-9.2.3-種-X1 101AS -9.2.3-SS-X1 生物技術課 Biotechnology Section 張惠如 Hui-Ju Chang (a) 重要蘭科花卉品種分子標誌技術開發：將成功大學、桃園場、臺南場及本場所開發之多組可應用於蝴蝶蘭(包括朵麗蝶蘭)商業品種之SSR引子，以24個共同試驗材料(12個原生種及12個商業品種)進行鑑別能力的篩選與測試。分析結果顯示有14組引子對具有較高的多型性與鑑別能力。並進一步以相似度高的20組品種權品種與對照品種進一步測試後，發現僅有大白花的旭東威士忌與V3無法鑑別，其他各組登記品種與對照品種，在單組或多組SSR分子標誌組合後，皆能被鑑別。未來將進一步由此14組SSR引子對中，挑選更佳之引子組合，並建立此分子鑑定系統的參考品種，並將試驗方法撰寫為成熟的標準試驗方法。(a-1)委託成功大學生物科學系吳副教授文鑾針對鑑別蝴蝶蘭（Phalaenopsis spp.）品種分子標誌技術進行整合。本年度利用一套共同的試驗材料，透過雙方共同測試比較SSR分子標誌之鑑別力，選出其中十組高可擴增率且高度鑑別力的SSR分子標誌，其PIC (polymorphism information content)值介於0.877 (NCKU4)至0.941 (NCKU3)之間。另外，分析十組SSR分子標誌出現頻率最高的61個對偶基因，並選定可涵蓋這些對偶基因的十個蝴蝶蘭品種，作為未來基因型鑑定流程的參考品種，以確認每次鑑定流程的結果可重複性。(b) 檢定人員訓練及植物品種權教育推廣：已於2012年10月31日舉辨「2012年作物新品種檢定講習會」，參與人數共計101人。本次研習會講題除介紹一些新作物檢定方法外，亦介紹「蝴蝶蘭品種影像辨識系統」及「數位影像編輯軟體GIMP」之使用方式，為應用於檢定作業及性狀表開發的輔助工具。另外，更以「蝴蝶蘭品種權之國際合作」一題，詳細的介紹並說明臺灣的品種權將以蝴蝶蘭為首開展國際合作，提供有興趣的業者相關資訊，也促進檢定人員了解國際合作的概念及發展方式。(c) 木瓜三倍體選育運用於品種保護研究：以上年度利用秋水仙素1.5%處理幼苗莖頂，以流式細胞儀測定認定為4倍體植株後，定植於田間，進行自交及雜交，其自交一代及雜交一代經流式細胞儀檢測獲得10株4倍體植株，再經壓片鏡檢只有1株確認為4倍體。 The projects of this program have three components. One of the components is to establish varieties identification through SSR molecular marker of new and/or commercial varieties of Phalaenopsis. Ten out of 33 polymorphic SSR markers developed from NCKU(National Cheng Kung University) TDARE(Taoyuan District Agricultural Research and Extension Station) TDARE(Tainan District Agricultural Research and Extension Station) and TSS (Taiwan Seed Improvement and Propagation Station) were selected through testing the transferability and discriminative power among 12 Phalaenopsis species and 12 Phalaenopsis cultivars and the PIC(polymorphism information content) ranged from 0.69 (Pm4077) to 0.94(NCKU2 NCKU3 NCKU9). The results showed that these 14 SSR markers can be used to complement the DUS test for identifying varieties with similar morphology effectively. The second is to training the faculty members to know the relative technologies of DUS test and system of plant variety protection through technique short-term training and keynote speech.The other one is tripolid papaya selection and improvement. Last year use of colchicines over 1.5% top of seedling stems by flow cytometry after the determination deemed to be tetrapolid of plant planting in the fields selfing and hybridization self-generation and their hybrids through flow cytometry detection. There were 10 plants multipolid and then confirmed by microscopy only 1 for tetrapolid.The results of this program will provide helpfully informations of plant variety protection.2012 植物種苗團隊-植物品種開發及種苗驗證之應用研究-植物種苗認驗證體系建構 Establishment of the Accreditation system for plant seedlings 101農科-9.2.3-種-X2 101AS -9.2.3-SS-X2 生物技術課 Biotechnology Section 莊淑貞 Su-Jean Chuang 1.種苗檢測技術認驗證TAF實驗室設備建構及維護：本年度實驗室之儀器建置與驗證。新購置儀器(包括核酸定量儀、聚合酵素梯度溫度連鎖反應儀、植物生長箱、微量分注器以及桌上型離心機)符合TAF檢測實驗室需求之確認及各廠牌測試比較，並已完成採購及驗收程序。完成實驗室已設置儀器(包括ELISA讀值儀、微量分注器、酸鹼度計及聚合酵素溫度連鎖反應儀等)之年度校正維護，以符合TAF檢測實驗室管理。2.瓜類退綠黃化病毒及草莓潛隱輪斑病毒驗證體系建構：參酌國內外文獻，已完成瓜類退綠黃化病毒（Cucurbit chlorotic yellows virusCCYV）與草莓潛隱輪斑病毒（Strawberry latent ringspot virus SLRSV）之檢測技術盤點。瓜類退綠黃化病毒目前檢測技術主要有：RT-PCR、Western blotting、ELISA及Immunoelectron microscopy；草莓潛隱輪斑病毒目前檢測技術主要有：RTPCR、IC-RT-PCR、ELISA與病徵判別。以不同的樣品進行各種檢測方法的方法測試，挑選最適合的檢測方法做為標準檢測流程。3.種子(苗)品質純度分子檢測技術研發及標準化：種子公司番茄品種植體材料(雜交一代及父、母本親)，及種苗場番椒新品種種苗2號及4號，共3組9個材料。番椒共篩選出8/100組有用引子，11個識別片段。番茄共篩選出7/100組有用引子，7個識別片段。經回收、定序、解序及重新設計條帶專一性引子，番茄SCAR標誌7組增幅出6個專一性條帶，番椒SCAR標誌10組增幅出6個專一性條帶。以番茄採種種子為材料，及以5個番茄識別標誌，進行F1種子純度分子檢測。檢測結果，顯示2個雜交種子其花粉為來自外來花粉非雜交種之父本親，本批番茄採種種子的F1種子純度為97.6%。4.十字花科種子健康檢查驗證體系之建立：本計畫目的係為準備種子檢查室十字花科種子黑腐病菌檢測認證工作，已依國際種子檢查協會規定之檢測方式建立適合本實驗室之十字花科種子黑腐病菌檢測流程。實際以人工汙染種子進行檢測流程的測試，可於FS及mCS20ABN兩種選擇性培養基上觀察到測試菌落的標準型態；在病原性測試也可將分離出之黑腐病菌菌株接種於甘藍葉片上顯示出典型病徵；靈敏度測試結果亦可測得達0.02%的帶菌率。 The purpose of this plan first is to promote the plant pathogens detection and isolation efficiency of the laboratory. This yearregarding TAF-certification for laboratory equipment design and layout of the laboratory has already been completed and laboratory bench and instruments have been acquired calibrated and approved for operations.Second purpose of this plan with reference to published literaturedetection methods for Cucurbit chlorotic yellows virus CCYV and Strawberry latent ringspot virus SLRSV have been researched and cataloged. Current detection methods regarding Cucurbit chlorotic yellows virus CCYV include: RT-PCR2012 RFID技術應用於種子倉儲管理之研究與開發 Study and development on RFID technology for the seed production and marketing management 101農科-7.3.1-種-X1 101AS -7.3.1-SS-X1 種苗經營課 Seed & Seedling Management Section 許鐈云 Ciao-Yun Syu 種苗場擁有9座種子冷藏庫，長年配合政府政策負責生產供應全省雜糧、綠肥及蔬菜作物種子，操作從契作生產及外購種子、調製、加工、倉儲、運輸至推廣銷售等一系列種子產銷流程作業。去(100)年度針對種子倉儲部分建構「種子倉儲管理系統」部分，並結合無線射頻辨識(RFID)應用，達成種子倉儲流程作業電子化，除可供本場內部良好管理，增加種子倉儲管理透明性及即時性，此外結合RFID監控倉儲溫溼度環境情況，同時亦減少種子損耗，降低購買者損失。本(101)年度擴大應用層面，於軟體系統方面將第一期開發的系統加以擴充，增加會計(含出納)功能。 Taiwan seed improvement and propagation station (TSS) has 9 seed　refrigerated storages and is responsible for the seed production of　cereal green manure and vegetable. Moreover there are a series of seed　producing and marketing procedure operation including producingpurchasing processing packaging storage transportation and marketing.In last year it’s aim to accomplishment of the efficient method of seed　storage management by RFID technology in order to maintain the seed of　quality to decrease the waste of manpower and time and to enhance the　check time of the inventory in the seed storage effectively. In this　year it’s important to extend the capability of the system including　accounting and cashier.2012 建立飼料玉米種子籌供體系 Establishment of hybrid corn production and supplying system 101農科-9.2.1-種-X1 101AS -9.2.1-SS-X1 農場 Research Farm 陳學文 Hsueh-Wen Chen 台南24號親本花期配合間隔（ASI），父本開花期較母本吐絲期約晚3.51天~5.92天；果穗結實粒數為粒552.06~591.42粒；而果穗結實率則介於85.6%~92.7%；台農1號親本花期配合間隔（ASI），父本開花期較母本吐絲期約晚0.44天~4.73天；果穗結實粒數為粒299.11~309.22粒；而果穗結實率則介於82.7%~94.67%玉米台南24號以栽植密度25公分之結實率最高，台農1號以栽植密度15公分之結實率最高。台中地區春作玉米台南24號及台農1號春作皆以栽植密度15公分之結實率最高。台中地區秋作因生育期尚未結束，依目前數據分析，台南24號親本花期配合間隔（ASI），父本開花期較母本吐絲期約晚3.8天~5.1天；台農1號親本花期配合間隔（ASI），父本開花期較母本吐絲期約晚1.4天~3.8天。101年春作計觀察12項飼料玉米品種，觀察結果以台南24號及103對銹病抗性最佳，葉枯病則各品種罹病情形相近。台農1號、2#及台南20號最早熟，104最晚熟。單株產量以104、933及008最高，台農1號最低。 The objectives was established appropriate planting date for seed production of hybrid corn Tainan no.24 and Tainon no25cm sowing was higher. The results of Tainon no.1 found that male parent anthering date later 0.44 to 4.73 days than female parent silking date. The numbers of corn grains were 299 to 309. The rates of seed set were between 93.7%-94.7% which the 15cm sowing was higher in Pingtung. The rates of fertility were between 82.7%-90.8% which the 25cm sowing was higher in Taichung.From the investigation of spring season the rates of seed set of Tainan no.24. was increased with the planting density increased and TN no.1 wasn’t in Pingtung.There are the rates of fertility of Tainan no.24.and Tainon no.1 were increased with the planting density increased inTaichung.We suggested that the indirect effects of planting density seed yield. It also shows that the overall performance of the crop non-single factor can be decided such as fertilizers applied planting time soil properties is also extremely important and this need to be further explored.The collection of international evaluation and utilization of twelve feed corn varieties were observed in the spring of 2012 the results showed that 103 and Tainan 24 had the best performance on rust resistance and leaf blight resistance are similar among varieties.TNG12012 雜糧作物種子調製倉儲技術改進研究 Improvement of Seed process and storage techniques 101農科-9.2.1-種-X2 101AS -9.2.1-SS-X2 種苗經營課 Seed & Seedling Management Section 洪建民 Chien-Min Hung 本研究所調查及紀錄調製過程中不同乾燥作業環境和種子水分變化與油耗量關係之數據，期望能建立視不同的乾燥作業環境需求，供現場操作人員判斷及採取最適乾燥操作策略，以達到最省能源、最短乾燥時間、最佳能源利用效率等研究目的。研究資料顯示：乾燥期間大氣日平均溫度範圍12.1O C～20.7O C；日平均相對溼度：59%～94%時，種穗水分含量由30.7%降至17.8%需72小時，油耗量1090公升。大氣日平均溫度範圍7.5～16.5 OC、日平均相對濕度58～96%時，種穗水份含量由30.5%降至18%需75.4小時，油耗量為1127公升。由研究資料得知，玉米種穗和種粒之減乾率和乾燥效能受乾燥期間大氣溫、濕度變化影響。 This research investigated the relationship of the operated environment in different dry conditions the changes in seeds moisture and the consumption of diesel oil. The object of the research was to establish the strategy of the best operation to dry seeds to short the energy and drying time. When the average temperature was 12.1-20.7 OC and the average RH was 59%～94% it cost 72hr to decrease the moisture of the corncobs and seeds from 30.7% to 17.8% and the consumption of diesel oil was 1090kg. When the average temperature was 7.5-16.5OC and the average RH was 58%～96% it cost 75.4hr to decrease the moisture of the corncobs and seeds from 30.5% to 18% and the consumption of diesel oil was 1127kg.The difference rate of moisture of the corncobs and seeds and efficiency of drying seeds were directly influence by the change of temperature and RH during the drying time.2012 優質番木瓜品種選育、採種及栽培技術改進 Good quality varieties selection and improvement the technology ofcultivation and seed production on papaya 102農科-9.2.2-種-X1 102AS -9.2.2-SS-X1 屏東種苗中心 Pingtung Seed & Seeding Research Center 邱展台 Chan-Tai Chiu 不公開 Not public2012 植物種苗團隊-蔬菜品種改良及採種技術研究 Study on improvement of vegetable varities and technology of seed production 101農科-9.2.2-種-X2 101AS -9.2.2-SS-X2 品種改良保護課 Plant Breeding Section 郭宏遠 Horng-Yeuan Kuo 本計畫之目的在育出高產量、高品質、耐熱和抗病之胡瓜、南瓜、苦瓜、番椒及茄子之新品種，並進行具潛力新品系之採種技術研究。 The main purpose of this project is to breed for promising varieties with desirable characteristic such as high yield superior quality heat tolerance and diseases resistance and to do the seed production tests for the potential new hybrids of cucumber pumpkin bitter gourd pepper and eggplant.2012 仙履蘭及熱帶球根花卉品種改良與種苗生產技術開發 Improvement on slipper orchid and tropic bulb flowers breeding and establishment the seedling productiontechnique 101農科-9.2.2-種-X3 101AS -9.2.2-SS-X3 品種改良保護課 Plant Breeding Section 劉明宗 Ming-Chung Liu 依照仙履蘭育種四大目標進行雜交授粉工作，101年雜交授粉9組合，本年挑選出多花及較耐熱性之親本進行雜交，但授粉成功機率低，有可能是亞屬間雜交及夏季溫室溫度較高之因；從仙履蘭雜交組合中以PA94002雜交組合後裔之不同單株具有Paph. primulinum品種多花特性，花朵會同時開放或者接續開放，且有的後裔也有單花的出現，因此可再從這些後裔中，篩選單花或雙花特性，並可同時開花為佳。球根花卉品種選育在彩色海芋方面，94及95年雜交後裔中已選出具潛力單株共4株，進行單株性狀比較，褐黑色系列中以品系95069具有花色特殊之優點，花莖較長之優點，單株95036-a是所有單株比較中不論花莖及花苞皆最長，且花苞形狀較適宜切花包裝。孤挺花新品種選育中，從孤挺花優良雜交單株進行性狀比較觀察，獲得具潛力單株4株，其中白色系以24X76表現最佳，花梗長約38.2公分，具有種球小即可順利抽梗之特性。單株D94001之花色表現較佳，為鮭魚色，重瓣化明顯，適合做盆花用途；單株H912003為紫紅色，顏色亮麗，可作為切花與盆花用途。以孤挺花新品種種苗一號-粉珍珠及種苗二號-紅豔之小鱗球為材料，進行開花球養成之肥培管理試驗，試驗結果顯示供試之不同施肥頻度在葉片數差異不大，但是葉長與葉寬方面則以高頻度施肥(每週施肥一次)似乎較佳，而對種球是否有影響，則必須待種球採收後才能得知。宮燈百合定植田間網室，進行不同肥料比例試驗，以N-P2O5-K2O﹦30-10-10之肥料比例，對於植株地上部之生育較佳；施用N-P2O5-K2O=20-20-20之肥料比例進行施肥濃度試驗，結果以肥料稀釋1000倍，對地上部生育較佳。 According to four goals in slipper orchids breeding we have selected superior progenies ‘PA94002’ with Paph. Primulinum’s multifloral characterestics. Bulbous flowers in calla lilies breeding during in 2005 and 2006 hybrids we have selected potential progeny of ‘95069’ with dark-purple spathe and good horticultural characteristics. It can be used as cut flower. Another potential progeny of ‘95036-a’ with long spathe and peduncle length and good spathe shape which is easy packed .Bulbous flowers in amaryllis we have selected 4 potential hybrid progenies of double-flower. The white line of ‘24X76’ the best performance of flower shape salmon color line of ‘D94001’ is better performance for the double tepals and purple-red color of ‘H912003’ is bright color of flower. For the fertilization frequency test in amaryllis of TSS No.1-Pearl and TSS No.2-Red Splendor the results show the different fertilization frequency is no significant difference on leaf number. The leaf length and width is better on high frequency fertilization.Sandersonia tubers were planted in soil under protected net greenhousewhich were tested different fertilized formula. The result is fertilized formula N-P2O5-K2O=30-10-10 better for sandesonia plants growth2012 植物種苗團隊-種子品質及處理技術研發 Developement for the seed processing techniques to promoting seed quality 101農科-9.2.3-種-X3 101AS -9.2.3-SS-X3 種苗經營課 Seed & Seedling Management Section 黃玉梅 Yu-Mei Huang 不公開 Not public2012 植物種苗量產體系建立 Establishment of mass production system of plant seedlings 101農科-9.2.3-種-X5 101AS -9.2.3-SS-X5 繁殖技術課 Propagation Technology Section 王至正 Jhih-Jheng Wang 不公開 Not public2012 植物種苗團隊-植物新品種檢定技術之開發與執行 Plant Seed Group-The Technical Development and DUS Test Execution ofPlant Variety 101農科-9.2.3-種-X4 101AS -9.2.3-SS-X4 品種改良保護課 Plant Breeding Section 安志豪 Chih-Hao An 本年度已完成草擬藍眼菊、觀音蓮及草擬修訂茼蒿品種性狀表與試驗檢定方法初稿。關於蝴蝶蘭及文心蘭等作物資料庫建立方面，已收集15個蝴蝶蘭及5個文心蘭商業品種並完成品種性狀調查及資料庫建置。本年度受理新品種性狀檢定工作計蝴蝶蘭與朵麗蝶蘭126件、文心蘭3件及玫瑰1件；正進行性狀檢定中之案件為蝴蝶蘭與朵麗蝶蘭30件及文心蘭2件；檢定完成資料整理中為蝴蝶蘭與朵麗蝶蘭20件；檢定完成且審查結束為蝴蝶蘭與朵麗蝶蘭37件、文心蘭2件、蕙蘭1件、捧心蘭1件及玫瑰1件。 The purpose of this project is to establish the test guidelines and execute DUS tests. We modified the test guideline and table of characteristics of osteospermum alocasia and edible chrysanthemum. We also collected 15 commercial varieties of phalaenopsis and doritaenopsisand 5 commercial varieties of oncidium and investigated their characteristics for establishing variety database. We accepted 126 cases of phalaenopsis and doritaenopsis 3 cases of oncidium and 1 case of rose to execute DUS tests for protecting the breeder’s rights this year. 30 cases of phalaenopsis and 2 case of oncidium have been executing DUS tests. 37 phalaenopsis and doritaenopsis 2 oncidium 1 cymbidium 1 lycaste and 1 rose have been granted to be new varieties2012 植物種苗團隊-亞太地區植物種苗產業研究與應用 Studying and application on Vegetable plant seed industry in Asia-Pacificregion 101農科-9.2.3-種-X6 101AS -9.2.3-SS-X6 技術服務室 Technical Service Section 周明燕 Ming-Yenn Chou 不公開 Not public2011 植物種苗團隊-植物育種研發成果移轉機制建構及媒合平台建置 Study on mechanism and platform for matching and transfer of COA's plant breeding achievements 100農科-4.2.2-種-X5 100AS-4.2.2-SS-X5 品種改良保護課 Plant Breeding Section 郭宏遠 Horng-Yeuan Kuo 1.強化植物育種半成品媒合平台及資料庫功能及整併至農業技術交易網由種苗場、中興大學、工研院及農業策進辦公室共同合作，將植物育種半成品媒合平台整併至農業技術交易網。目前試驗良場所育種人員可至成果網(http://agriti.coa.gov.tw/User/PlantHalfFinishList.aspx)修正確認資料並作正式上線前之測試。2.充實半成品資料庫內容由農委會所屬各研究單位提報具發展潛力之半成品資料共30筆，上傳供農業技術交易網進行先期功能測試之用。3.教育訓練本年7月18日於種苗改良繁殖場舉辦半成品媒合平台推廣說明會。會中介紹本計畫執行內容，並展示網站合併後之介面與使用說明，與會人員充分交流，提供修正之建議。4.訪談種苗協會，並舉辦半成品媒合平台座談會本年10月19日與種苗改進協會施理事長訪談，討論業者對半成品媒合平台之意見，12月7日舉辦半成品媒合平台座談會，邀請試驗改良場所與種苗業者，研商植物育種半成品商品化之連結。5.半成品材料移轉合約草稿完成半成品材料移轉合約草稿乙份，可供未來進行半成品移轉參考之用。 1.Plant Materials Matching Platform website up-graded and emerged With the assistance of Taiwan Seed Improvement and Propagation Station (TSIPS) the team of Law Department of National Chung-Hsing University (NCHU) Industrial Technology Research Institute (ITRI) has successfully emerged the NCHU developed platform to the Agriculture TechnoMart of ITRI. All member researchers can now test upload and edit their data Through the following link : (http://agriti.coa.gov.tw/User/PlantHalfFinishList.aspx)2.Data collectionAll research institutions of Council of Agriculture provide their potential plant material data to TSIPS on their demand and then transfer data to NCHU and ITRI for uploading and collecting. Then the researchers can check the data and test the function before formal operation next year.3.Training sessionThe team of NCHU held a training session at Taiwan Seed Improvement and Propagation Station on July the 18th. The session began with the introduction of the research project and then the instruction of the Plant Material Matching Platform and ended with an open Q＆A.4. Interview and SeminarThe team interviewed the chairman of Taiwan Seeds Trade Association to discuss about plant materials and the platform. After the interviewTSIPS and the team hold a seminar at December 7th to connect research institutes and commercial enterprises. 5.Trasfer Agreement Draft Completed drafting the plant materials transfer agreement2012 作物遺傳種原收集、保存與利用 Preservation collection and application of germplasm 101農科-9.2.3-種-X7 101AS -9.2.3-SS-X7 品種改良保護課 Plant Breeding Section 林正雄 Cheng-Hsiung Lin 1.完成82個豇豆品種、10個西瓜品種及25個萵苣品種等，合計117個蔬菜種原之更新與繁殖。2.本年度蜜雪梨收穫量為6140.7公斤，果品糖度平均為10.8Brix，較歷年來偏低，推測原因為今年採收前颱風及豪大雨所致造成蜜雪梨果品糖度及品質不如歷年來的好。3.收集20種本省原生綠化苗木，每品種至少保存20~50株，植株生育健壯後，再調查每品種生長狀況及性狀等以待品種保存用。 1. We had renewed 25 species of lettuce 82 species of cowpea and 10 species of watermelon in this year. In the meanwhile we had completed the characteristic investigation of all.2. Not only maintained the original parent species of Tainung No.2 pear but had harvested 6000Kg fruits in this year.3. We had collected 20 original species of tree this year and had propagated 20 trees per species at least.2012 台灣香藥草植物資源開發利用 Development and utilization of indigenous aromatic herbs resources in Taiwan 101農科-9.2.3-種-X8 101AS -9.2.3-SS-X8 繁殖技術課 Propagation Technology Section 羅英妃 Ying-Fei Lo 不公開 Not public2012 有機農業團隊-蔬菜種子有機種衣劑與種子有機處理基準之研究 Establishment of vegetables organic coating material and organic processing benchmark in seedling 101農科-9.2.4-種-X1 101AS -9.2.4-SS-X1 種苗經營課 Seed & Seedling Management Section 黃玉梅 Yu-Mei Huang 1. 以高嶺土：滑石粉1：1混合後添加一層機能性資材於茄科種子以紙上法進行發芽試驗在下列濃度下不影響發芽率：番茄種子施用75%甲殼素、25%木黴菌及海草粉，茄子種子施用全量甲殼素、木黴菌及75%海草粉，甜椒種子施用全量甲殼素、75%竹碳粉、木黴菌及50%苦茶粕、海草粉，辣椒種子施用全量竹碳粉、75%甲殼素、苦茶粕、木黴菌及50%海草粉。但以高嶺土加滑石粉做為底衣粉配方結果顯示在茄子、甜椒及辣椒上皆會影響發芽天數，因此另以麥飯石粉及皂土為底衣粉配方可縮短發芽天數，但與未披衣及60℃烘乾20分鐘之種子做對照，結果顯示目前的烘乾溫度會明顯影響茄子平均發芽天數，故未來將以不同烘乾方式減少對披衣種子發芽之影響，其苗期試驗仍在進行中。另外，萵苣種子以高嶺土：滑石粉3：1做為底衣粉及10%阿拉伯膠為底衣液時不影響發芽率，未來將進一步進行添加一層機能性資材之試驗。2. 溫度等九種之種子物理處理中技術與資材應符合現行有機規範，而滲調常使用之鹽類如聚乙烯二醇(PEG)並不符合。溫湯處理55℃明顯降低十字花科萵苣種子發芽率，溫湯處理60℃明顯降低六種十字花科種子發芽率，乾熱處理606570℃則不影響種子發芽率。溫湯處理60℃明顯降低茄科辣椒種子發芽率，乾熱處理606570℃則不影響種子發芽率。十字花科甘藍與青花菜種子經接種黑腐病菌(Xanthomonas campestris pv.campestris.)後，乾熱處理606570℃後培養基上的種子仍有菌落的產生，但不影響其發芽率。 Solanaceae seeds were first coated with kaolinite and talc powder mixed of 1:1 and then added one layer of functional materials. When added 75% chitosan25% Trichoderma and seaweed powder in tomato seeds; 100% chitosan Trichoderma and 75% seaweed powder in eggplant seeds; 100% chitosan75% biocarbon powder and Trichoderma 50% tea seed pomace and seaweed powder in sweet pepper seeds; 100% biocarbon powder2012 作物種苗病害檢測、驗證及防治技術之開發與應用 Application and development of detection certification and control of crop diseases 101農科-10.3.1-種-X1 101AS -10.3.1-SS-X1 繁殖技術課 Propagation Technology Section 袁雅芬 Ya-fen Yuan 本年度已取得15個南瓜品系共216個樣本進行白粉病原菌接種試驗；同時自不同地點收集白粉病原菌進行繁殖與保存；並建立南瓜對白粉病抗性之病害等級；目前接種的品系中，僅少數品種為可能較具抗白粉病特性之品種。以濃度為108CFU/ml的西瓜果斑病菌汙染乾淨的西瓜種子，發芽後種子置床於72穴穴盤中，並以不同編號的根圈微生物菌液，澆灌處理上述接種的西瓜幼苗，篩選出的拮抗細菌編號15-7、15-8、15-9及15-10可使西瓜幼苗存活率較對照組增加3倍，可做為生物農藥開發之用。委託廠商依序列合成之馬鈴薯病毒peptide經Western blotting 反應無法以商業生產之抗血清檢測，仍須以病毒鞘蛋白全長序列選殖，並誘導表現之重組蛋白生產血清較佳。胡瓜栽培期間以亞磷酸溶液處理，調查對露菌病防治之影響，由調查結果顯示，使用亞磷酸溶液500倍與1000倍噴施皆具有減緩或抑制露菌病發生的效果，但以亞磷酸溶液500倍處理之效果優於亞磷酸溶液1000倍，且以連續噴施對抑制胡瓜露菌病發生的效果較為持久。而在使用窄域油300倍、500倍及苦棟油300、500倍對於葉蟎發生率的調查結果顯示，雖然以窄域油及苦棟油處理對於葉蟎發生率皆具有抑制的效果，但防治效果較為短暫。 Fifteen different pumpkin hybrid lines were screened to the powdery mildew -resistance which inoculum pathogen species. According the inoculum results showed that the virulence were different by the host hybrid lines. There were a few of hybrid lines resistant to the powdery mildew. We construct a grade of the powdery mildew –resistance in pumpkin.Watermelon seeds inoculated with 108CFU/ml Acidovorax avenae subsp.citrulli were planted in 72-cell plug after germination. The seedlings were poured with culturing media of different rhyzosphere bacterial. By this method 4 antagonist bacterial : No. 15-7 15-815-9 and 15-10 were screened. They made the survival rate of watermelon seedlings increasing 3 times than the control. These bacterial may be used to develop microbial pesticides.The amino acid sequences of potato viruses coat protein were analyzed by a contractor. However the synthesized peptides were not detected by western blotting with commercial antiserum anti PVS PVX PVY. It is necessary to clon full-length coat protein genes of viruses. Then inducing expression of recombitant proteins for antiserum production is better.The cucumber treated with different concentrations phosphorous acidinvestigated the effect for the downy mildew during cultivated period.Result showed that the different concentrations phosphorous acid was effective on the cucumber downy mildew. The phosphorous acid applied at 2g/l was better than at 1g/l and the effect was better while applying continuously. The spider mites could be control by treated with the narrow range oil(2㏄/l 1㏄/l) and China-berry oil(2㏄/l2012 花卉團隊-生產蕙蘭無特定病毒之種苗檢測系統之建立 The establishment of detection system of Cymbidium Specific-Pathogen-Freeseedling 101農科-10.3.1-種-X2 101AS -10.3.1-SS-X2 繁殖技術課 Propagation Technology Section 邱燕欣 Yen-Hsin Chiu 為防止蘭花病毒藉由蕙蘭種苗散佈蔓延，以ELISA法檢測種苗業者生產之蕙蘭種苗病毒ORSV、CymMV之發生率，期能提升蕙蘭種苗之品質，以維護消費者的權益，並進一步提升蕙蘭種苗內外銷市場競爭力。 To prevent virus diseases from spreading through Cymbidum seedlings we plan to drive and carry out a health certification system for Cymbidum seedlings which is expected to improve the quality and the market competition strength of Cymbidum seedlings for furthur export trade.2012 植物種苗團隊-動植物有害生物診斷鑑定技術及種子苗病害驗證技術之研究與應用 Establishment and Research of Seed and Seedling Certification Techniques and Regulations 101農科-10.3.1-種-X3 101AS -10.3.1-SS-X3 繁殖技術課 Propagation Technology Section 邱燕欣 Yen-Hsin Chiu a.符合目標市場外銷國之種子苗驗證技術盤點與研發文獻搜尋並比較國際試驗室間檢測番茄嵌紋病毒(Tomato Mosaic Virus，ToMV)的檢測方式保括ELISA、RT-PCR、ICRT-PCR等檢測技術，本計畫前期合成針對ToMV專一之引子對以及植體之Internal control primer set 可建立RT-PCR偵測受檢檢體內之ToMV，確定檢測之正確率。101年擬定檢查流程文件-番茄植體檢測番茄嵌紋病毒作業標準一式，並通過財團法人全國認證基金會(Taiwan Accreditation FoundationTAF)技術認證。b.外銷重要蔬菜番茄及甜椒種子處理技術之研究本計畫利用氯化鉀電解水、熱水及殺菌劑等方法，研究最適處理條件進行種子滅菌處理，以減少茄科細菌性斑點病之種傳情形與其初次接種源。以HClO濃度50 ppm以上的氯化鉀電解水處理5分鐘以上即可有效除滅甜椒種子與番茄種子上的病原菌；45°C熱水處理30分鐘以上可明顯降低甜椒種子上的帶菌情形，甚至50°C熱水僅處理10分鐘即可完全除滅甜椒種子上的病原菌，同時不降低種子發芽率。殺菌劑試驗中以0.6%漂白水滅菌效果最好，而嘉賜銅處理，除有效降低帶菌情形、甚至完全除滅病原菌外，同時也不會降低種子的發芽率。c.外銷蔬菜種子品質提升技術研究 (委辦)本研究的目的在開發不影響種子發芽率之種子殺菌技術，以防治十字花科黑腐病。應用100年度建立之條件『3% H2O2浸漬30秒再以70℃熱風烘乾種子30分鐘』處理1Kg天然帶黑腐病菌種子，結果顯示雖然可以完全殺滅黑腐病菌，但種子發芽率由80%降低至45%。考慮種子原先品質對試驗結果之影響，本年度改用發芽率95%以上之花椰菜及甘藍菜種子，經Xanthomonas campestris pv. Campestri (XCC1-1 strain)污染作為本試驗帶菌種子材料，殺菌處理分別為：『3% H2O2浸漬30秒再以70℃熱風烘乾種子30分鐘』、經70oC乾熱處理7天，及熱硫酸鋅，種子處理後分裝儲藏於防潮箱，每月追蹤其發芽率及帶菌情形。結果顯示所有處理對發芽率皆無顯著不良影響，發芽率穩定維持在95%以上，滅菌效果則以70oC乾熱處理7天為最佳，連續6個月種子洗出液之XCC1-1皆維持零檢出，其他處理之殺菌效果較不完全、也較不穩定。因此，本試驗的結論是『70oC乾熱處理7天』處理為最佳條件方便於大量種子處理，對發芽後兩週幼苗之外觀沒有影響，但估算其狀苗指數，對甘藍沒有影響，但對花椰菜則會降低，因此後續對成株之影響仍需評估。d.建立重要蘭花病毒ORSV、CymMV新檢測技術建立胜肽親和性篩選系統(peptides 12)對於標地蛋白之親和性汰選技術，建立及生產與CymMV病毒鞘蛋白具親和性之噬菌體，篩選出可利用於ELIS檢測CymMV之噬菌體。e.建立苦瓜種子發芽檢查程序研究 (委辦)苦瓜種子種皮厚且硬，會妨礙水分吸收及氣體交換，發芽過程中常有發霉的現象，造成種子發芽率不佳及不整齊等問題。本實驗以四品種苦瓜為研究材料，結果顯示苦瓜種子發芽最適溫度為30℃，最適發芽前處理為60℃溫湯浸種10分鐘，並且以紙間法加入4 c.c.發芽水促進發芽效果最佳，可提高苦瓜種子發芽率80%以上。為符合國際ISTA規範，將苦瓜種子依照發芽率高、中、低三等級，委請三個實驗室進行發芽試驗。統計分析三個實驗室發芽數據確定三批發芽活力之差異性未超過容許度，顯示所建議苦瓜種子發芽前處理之方法可推薦給ISTA之種子檢查規則使用。 a. 符合目標市場外銷國之種子苗驗證技術盤點與研發By papers literature research we selected the RT-PCR as the major tech as our TAF testing method for Tomato Mosaic Virus. At the first we design a set of RT-PCR primer for ToMV specific primer set. We also design the internal control primer set for more reliable detection result. In 2012the stand operation procedure of ToMV detection of Taiwan Accreditation Foundation Taiwan Accreditation Foundation (TAF) was established. ToMV Detection of Tomato leaf in-House Method(Document No.:No.WI33).b. 外銷重要蔬菜番茄及甜椒種子處理技術之研究The methods including electrolyzed KCl water solution hot water and bactericides were adopted to study the seed disinfection treatments for decreasing the seed transmission and primary inoculum of bacterial spot of tomato and sweet pepper. The electrolyzed KCl water solution with fifty ppm and above of HClO concentration were effectively to disinfect the artificial infested sweet pepper and tomato seeds that were treated five and above minutes. The treatments of thirty and above minutes with 45°C hot water obviously decreased the Xv-carried ratio of artificial infested sweet pepper seeds. The treatment of ten minutes with 50°C hot water could totally disinfect the Xv on artificial infested sweet pepper seeds and meanwhile that did not reduce the germination rate. In the trial of varied bactericides the treatment with 0.6% sodium chloride showed the best disinfection on artificial infested sweet pepper seedsand the treatment with Kasugamycin and copper oxychloride could effectively reduce Xv-carried ratio and even thoroughly disinfect the artificial infested sweet pepper seeds without diminishing the germination rate.c. 外銷蔬菜種子品質提升技術研究 (委辦)The objective of this study was to establish a disinfection method for seeds of Crucifer vegetables for preventing black rot disease caused by Xanthomonas campestris pv. campestri (XCC). As applied the previous established protocol of ‘ 30 seconds of 3% H2O2 followed by heated dry at 70oC for 40 min’ to large scale experiment (1 Kg seeds) could completely disinfect the pathogen carried seeds; however2012 應用綠肥營造環境親和型水旱田輪作經營模式 The establishment of environmental friendly crop rotation model using green manure 101農科-14.1.2-種-X1 101AS -14.1.2-SS-X1 種苗經營課 Seed & Seedling Management Section 鄭梨櫻 Lee-Ying Cheng 為降低氣候變遷對台灣飼料玉米生產之衝擊，調整玉米品種並利用輪作綠肥設計低投入環境親合型輪作模式，評估各模式對土壤環境及農民收益之效力。試驗結果，輪作綠肥可提升土壤有效性氮含量並降低土壤總體密度，所增加之氮素可讓後續作物減施化肥，甚而因土壤肥力改善而提高後續水稻產量，雖然減少一個期作現金收入而降低收益，惟其整體效益仍待後續評估。 To reduce climate change impact on Taiwan feed corn production adjust the corn varieties and use green manure to design low-input environmental friendly crop rotation model assessing the effect of modes on soil environment and farmers ' income. The results indicated green manure rotation will increase effective nitrogen content in soil and reduce soil density the increase of nitrogen for subsequent crop fertilizer reduction even increased follow-up of rice production due to improved soil fertility.Although one less as cash income while reducing total income but their overall benefits remain to be follow-up assessments.2012 冬作耐溼馬鈴薯品種選育 Wet resistant breeding for potato in winter 101農科-14.1.2-種-X2 101AS -14.1.2-SS-X2 品種改良保護課 Plant Breeding Section 薛佑光 Yu-Kuang Hsueh 已由國際馬鈴薯中心(International Potato Center，ICP)獲得之雜交種子繁殖二千株實生苗，並從中選拔具有生長勢強、種薯產量高及品質佳之營養系48個，進行冬季耐濕品系選拔。100/101年期冬作馬鈴薯耐濕試驗，以低溫及高濕環境作為汰選方法，經調查及評估48個營養系，在生長勢、生育中期之株高及葉色、葉綠素含量、生育後期之罹病與倒伏比例、採收後單株薯重等結果，馬鈴薯代碼為1 8 3、91N80、470、91A179、T75、462、317等7個參試營養系具有最佳的表現。擬參考本次初步汰選資料，持續進行選拔與評估。 We selected the 48 clones by the International Potato Center of two thousand hybrid seedlings which are strong growth potential high tuber production and good quality. We selected better growth tuber yield and quality clones on wet and low temperature in winter. In this project we used the low temperature and high wet environment to investigate 48 clones. The potato number are 183 91N80 470 91A179 T75 462 317etc. They have the better growth potential2011 植物種苗團隊-種子品質及處理技術研發 Developed the seed processing techniques to promoting seed quality 100農科-4.2.3-種-X1 100AS-4.2.3-SS-X1 種苗經營課 Seed & Seedling Management Section 黃玉梅 Yu-Mei Huang 不公開 Not public2010 植物種苗團隊-符合外銷國之種子苗驗證技術研發、制度研究與建置 Establishment and Research of Seed andSeedling Certification Techniques and Regulations 99農科-9.2.1-種-X2 99AS-9.2.1-SS-X2 繁殖技術課 Propagation Technology Section 鍾文全 Wen-Chuan Chung 不公開 Not public2012 氣候變遷對授粉昆蟲行為模式與生態影響及生產因應對策-設施內茄科蔬菜利用蜜蜂授粉生產之研究 Study on influence of climate changing on the pollinator behavior modelecological impacts and produce countermeasures-Study on honeybee pollinaiton for Solanaceae vegetable production in the greenhouse 101農科-14.1.2-種-X3 101AS -14.1.2-SS-X3 品種改良保護課 Plant Breeding Section 郭宏遠 Horng-Yeuan Kuo 於密閉網室內施放蜜蜂進行番茄果實生產時，蜂箱需於第一花序開花前一星期放入網室內使蜂群馴化適應。網室內之溫濕度影響蜜蜂離巢及訪花之頻率。蜜蜂授粉之番茄果實生長速率高於自然授粉者，以授粉後3-4週內尤其顯著。蜜蜂授粉處理之果長、果寬、果肉厚度、單果重及種子數均優於自然授粉者，僅糖度略低。 Honeybee hive should be put into the net-house one week before blossom of first inflorescence for honeybee acclimation when tomato fruit production in the close house. Temperature and relative humidity in the net-house would affect the honeybee activities of leaving hive and foraging. In addition the growth rate of tomato fruit of honeybee pollination treatment was better than that of self-pollinated especially during the 3-4 weeks after pollination. Furthermore the fruit length fruit widthflesh thickness fruit weight and seed numbers per fruit were better than those of self-pollinated besides sugar of fruit2012 組織培養節能設備及技術之開發 Energy-saving equipment and technology development for tissue culture 101農科-14.1.5-種-X1 101AS -14.1.5-SS-X1 繁殖技術課 Propagation Technology Section 文紀鑾 Chi-Luan Wen 本計畫結果如下1.在培養室中建立一套10種不同型式(T5- fluorescent lamps)之光線控制系統。2.利用3種不同不週期調查對2種彩色海芋(cv. Florex and Pacific pink)及一種石斛蘭對芽體增殖之影響。彩色海芋之芽體置於3種不同光週期(8、12、16-h光期)，30天後，2種彩色海芋在12及16-h光期下培養之芽體高度高於8-h光期，Florex and Pacific pink芽體增殖倍率在12及16-h光期下培養明顯高於8-h光期。芽體鮮重與乾重在8-h光期下明顯降低，在12-h及16-h二種光週期比較下在芽體高度、芽體增殖倍率、鮮重及乾重均不影響。在石斛蘭(cv. Taiseed Tosnobile)上，在8-h及12-h二種光週期下培養芽體高度、芽體增殖倍率、鮮重及乾重均會明顯比在16-h光期下降低。3.在2種彩色海芋發根培養於塑膠培養瓶中以單層或雙層封膜試驗中，雙層封膜可顯著降低瓶苗之污染，而且雙層封膜不影響芽體高度、發根的數量及芽體鮮乾重。 The results of this program are examined see below:1. Set one auto-control system of 10 different lights (T5- fluorescent lamps) for tissue culture in culture room.2. The effects of 3 different photoperiods on shoot proliferation from 2 calla lily cultivars (cv. Florex and Pacific pink) and 1 dendrobium micro-shoots were tested. Shoot cluster of calla lily were cultured in culture room under 8 12 and 16-h photoperiods. After 30 d The shoots of 2 calla lily cultivars cultured under the 12 and 16-h photoperiods were taller than those grown under 8 h. The shoot proliferation rate of Florex and Pacific pink increased when plants were grown at 12 and 16-h photoperiods. Reduction in fresh and dry weights of shoots was significant already under 8-h photoperiods. 12 and 16-h photoperiods had no significant in shoot height shoot proliferation rate fresh and dry weights. In dendrobium (cv. Taiseed Tosnobile) Reduction in shoot heightshoot proliferation rate fresh and dry weights of shoots was significant under 8-h and 12-h photoperiods.3. In this experiment of sealing with membrane for tissue culture. 2 calla lily cultivars (cv. Florex and Pacific pink) rooting culture by plastic vessels and sealing with one or two layer membrane. Reduction contamination was significant by plastic vessels sealing with two layer membrane. Furthermore sealing with two layer membranes had no significant in shoot height the number of rooting fresh and dry weights.2012 健康種苗生產供應與健康管理體系之建立 Establishment of health seedlings production and health management system 101農科-14.2.2-種-X1 101AS -14.2.2-SS-X1 繁殖技術課 Propagation Technology Section 王至正 Jhih-Jheng Wang 不公開 Not public2011 植物種苗團隊-作物特定性狀分子標誌建立及選殖 The establishment and application of specific molecular markers for the crops species 100農科-1.1.1-種-X1 100AS-1.1.1-SS-X1 生物技術課 Biotechnology Section 莊淑貞 Su-Jean Chuang a.番茄抗斑點萎凋病毒基因型之分子鑑定：番茄斑點萎凋病毒病，為番茄溫室栽培的主要病害之一，近年更有蔓延的趨勢。危害症狀變化大，從苗期到成株各部位均可感染呈現症狀， Sw5抗性基因控制下接種TSWV的反應呈現無病斑或僅限制性過敏反應。本試驗完成在Sw5基因位置上建立分子標誌並利用F2族群分析抗性基因之分離情形，結果栽培種中之Sw5a與Sw5b抗性基因表現連鎖關係，分離比為1:2:1並已運用於大量樣品基因型檢定。b.木瓜性別相關基因及其分子標誌之建立：由DNA層次進行分子標誌的篩選工作，希望能獲得與木瓜全兩性性狀相關之分子標誌。經過多組的SSR及RAPD引子PCR試驗篩選，電泳分析發現：目前所設計之SSR引子對在各試驗材料間，並無篩選出具差異性的條帶。利用RAPD-PCR試驗分析，試驗結果發現有一組UBC-726引子在具全兩性性狀之(A1-3+A1-4+A1-5+A1-7)、(A68-6+A68-7)與不具全兩性性狀之 A1-1 、(A68-4+A68-5)的試驗材料間具有差異性條帶，未來將進一步分析此差異性條帶的序列資料與鑑別性確認。c.番椒抗炭疽病基因型之分子鑑定：以種苗公司提供之番椒品種，選定同一父母本後代之抗病性及感病性的番椒之葉片及果實，萃取蛋白質進行二維電泳分析，篩選出差異性蛋白質點期望作為分子標誌。目前已將於約19kDa處發現的蛋白質點（感病品種的特有而抗病品種沒有），重複進行數次二維電泳分析，將此蛋白質點取出送至生技公司進行定序工作，並已比對出此蛋白質點為抗、感病的相關基因PR10。 The projects of this program have three components including.The first is the establishment and application molecular markers of the resistance to sport wilt virus in tomato analysis system. An allelicspecific markers SCAR-Sw 5-1313 and SCAR-sw 5-676 were developed for differentiating allelic variation at Sw5 locus. It amplifies fragmentsfrom both resistant and susceptible tomato accessions with different molecular sizes that corresponding to the presence of Sw5/Sw5 Sw5/sw5 and sw5/sw5 alleles. Sw5a and Sw5b were resistance gene to sport wilt virus in chromosome 9 of tomato. Genes (Sw 5a and Sw 5b) could be identified by molecular markers Tss#SW5a and Tss#SW5b respectively. F2 segreation rate of Sw5/Sw5 Sw5/sw5 and sw5/sw5 was 1:2:1.The second is to establishment the molecular markers for sex determination in Carica papaya (papaya) by using RAPDand SCAR analysis.Marker Napf76/77-800 was specific to hermaphrodite and maleness. MarkerCFw/CRv-700 was specific to female.Marker Ypa7F/Ypa7R-1000 was specific to maleness. The markers could be applied for sexual identification in papaya seedling stage.The third is studing in multi-genes of agricultural anthracanose disease resistance in Capsicum spp by proteiomic analysis using twodimention electrophoresis. We utilized 2-D electrophoresis to screen the variational point of proteins between anthracnose- resistant and susceptible line extracted from the leaves and fruits of hot pepper.Partial point sequencing has been accomplished and we are currently undergoing protein type analysis and sequence conversions to establish the molecular markers differentiating anthrancnose-resistant lines.2011 植物種苗團隊-作物種苗微體繁殖技術之開發與改進 The study of healthy seedling production by tissue culture 100農科-1.1.1-種-X2 100AS -1.1.1-SS-X2 繁殖技術課 Propagation Technology Section 文紀鑾 Chi-Luan Wen 不公開 Not public2011 植物種苗團隊-仙履蘭產業技術開發及應用 Paphiopedilum industry technologydevelopment and application 100農科-1.1.1-種-X3 100AS-1.1.1-SS-X3 生物技術課 Biotechnology Section 廖玉珠 Yu-Ju Liao 不公開 Not public2011 植物種苗團隊-基因轉殖作物種苗檢測監測體系之建立 The establishment of detection and monitors system for genetically modified seedling 100農科-1.1.6-種-X1 100AS-1.1.6-SS-X1 生物技術課 Biotechnology Section 張珈琦 Jia-Ci Chang 已蒐集基因轉殖玉米種苗Bt11、E176、GA21、MON810、NK603、MON863、TC1507、T25等8個品系，並構築PCR檢測片段至核酸載體為標準樣品。基因轉殖大豆Roundup Ready 品系參考樣品，並建立標準檢測流程。另嘗試開發以螢光偵測分子儀建立基因轉殖作物nptII gene快速檢測方法，初步試驗結果顯示，應用螢光偵測技術可大幅縮短檢測時間，1分鐘內即可得到結果，唯結果判讀上，雖基因轉殖作物之螢光值可達非轉殖作物2倍以上，然尚無法穩定。在執行基因轉殖作物種苗TAF檢測實驗室方面，本(100)年度6月份確認通過延展評鑑，認證有效期至103年7月21日，11月份完成TAF增項申請，將基因轉殖玉米檢測、西瓜果斑病菌檢測、十字花科黑腐病菌檢測和海芋病毒檢測等4項納入ISO/IEC 17025檢測項目，並於12月份完成本年度認證實驗室內部稽核和年度管理審查會議。另外，已完成實驗室電腦條碼管理系統軟體升級和套裝化。本年度亦配合農糧署辦理一場基因轉殖作物檢測討論會。 The increasing presence of transgenic plant in the international markets has provoked a strong demand for appropriate detection methods to evaluate the existence of transgenic plants. The major objective of this study was to establish a detection technique for the GM maize soybean and broccoli. The events of GM maize are Bt11 E176 GA21 MON810 NK603MON863 TC1507 T25 etc. The event of GM soybean is Roundup Ready Soy.For this purpose some screening methods based on target gene and select marker was development. The results showed that the GM crops could be detected through PCR-based markers. We constructed the reference materials of GM maize with the PCR products. And we prepared the reference materials of GM soybean with seed powder. The fluorescent detector could be detected the nptII gene in 1 minute. In addition our aboratory has acquired TAF accreditation and increased four detection projects. And establish the computer management system for laboratory records management.2011 植物種苗團隊-基因轉殖及非基因轉殖種苗驗證及共存體系之建構 Construct the certification and Coexistence system for GM and Non-GM seedlings 100農科-1.1.6-種-X2 100AS-1.1.6-SS-X2 生物技術課 Biotechnology Section 沈翰祖 Han-Tsu Shen 基因轉殖作物檢監測體系由農糧署委託種苗改良繁殖場邀集農業試驗所、桃園區農業改良場、台南區農業改良場、花蓮區農業改良場、國立中興大學等單位建立「基因轉殖作物檢測監測小組」，藉由「檢測技術建立」、「儀器與檢測精準度試驗」、「檢測樣品檢出能力試驗」、「能力試驗」等，已建立抗輪點病毒病木瓜、雙重抗木瓜輪點病毒及木瓜畸葉嵌紋病毒性狀基因轉殖木瓜、衛生署公告許可之上市品項之基因轉殖玉米及大豆等建立標準化檢監測模式，並利用multiplex PCR技術進行基因轉殖木瓜檢測，可在1次PCR反應中同時檢測papain gene、nptII gene、py16-cp gene與prsv-cp gene。其中基因轉殖木瓜及大豆種苗場已依據國際品保標準I S O / I E C 17025取得「財團法人全國認證基金會（Taiwan Accreditation Foundation TAF）」之生物領域測試實驗室認證，建立基因轉殖作物種苗認驗證體系。另蒐集國內外核酸定量檢測之量測不確定度(measurement uncertainty MU)、國內外基因轉殖玉米與大豆花粉飄散與傳統農業共存(coexistence)栽培模式研究成果，建立基因轉殖作物標準化取樣及監測模式並以田間試驗模擬基因轉殖玉米飄散趨勢，建立預測模式。亦配合種苗改良繁殖場利用衛星定位(GPS)調查台灣玉米、大豆栽培區資料，以地理資訊系統(Geographic Information System GIS)分析是否受到污染，並完成 GIS 規劃報告及 舉辦「基因轉殖作物生物安全座談會」。 The detecting and monitoring system of transgenic crops in Taiwan was entrusted by the Council of Agriculture which invited Agricultural Research institute Taoyuan District Agricultural Research and Extension Station Tainan District Agricultural Research and Extension StationHualien District Agricultural Research and Extension Station NationalChung Hsing University and Taiwan Seed Improvement and Propagation Station to form the team of detecting and monitoring transgenic crops. By means of accuracy detection capability and ability test the standard detecting and monitoring model have already been built up. In additionthe technique of multiple PCR was employed to detect transgenic papaya.The papain gene2011 植物種苗團隊-種苗生技廠商企業化經營輔導 Guiding agro-biotech manufactor of phalaenopsis for mass production of healthy seedling 100農科-1.1.8-種-X1 100AS-1.1.8-SS-X1 生物技術課 Biotechnology Section 廖玉珠 Yu-Ju Liao (一)聘請經營管理顧問針對無菌操作台10台以上之組織培養業者。輔導業者建立標準化生產作業程序：建立植物組織培養生產作業基本條件，包括作業程序、硬體設備、環境與設備維護等。輔導業者於組織培養生產作業的基本條件下建立自己的作業標準程序及落實管理文件化。（二）輔導業者提升蝴蝶蘭種苗品質：協助業者篩檢蝴蝶蘭分生苗母本病毒。1.以ELISA及RT-PCR檢測蝴蝶蘭病毒及協助業者建立無病毒母瓶。2.病蟲害監測及防治服務，蝴蝶蘭瓶苗出瓶後移植至開花苗階段之病蟲害監測記錄及防治諮詢。專案輔導5家無菌操作台10台以上之組織培養業者，提昇經營管理效益、產程作業管理、種苗品質，提升全方位競爭力。 The plan object is to match with the mission of bio-technique industry instruction group of Administration Yuan which is to promote development of bio-technique industry. The promotion objects are orchid seedlings growers with enterprise production scale. The instruction cases are aim at healthy management of maternal stock production procedure and quality control mechanism of healthy seedling seedling patent and international marketing etc. And hope to expand the scale of enterprise and increase product profits then achieve the object to promote successful investment cases.2011 植物種苗團隊-植物品種檢定流程分析、人員訓練及分子標誌開發 plant seed/seedling team – Analyzing the examining process flow training faculty members and developing molecular marker of plant variety right 100農科-1.1.9-種-X1 100AS-1.1.9-SS-X1 生物技術課 Biotechnology Section 張惠如 Hui-Ju Chang (一) 建立蝴蝶蘭新品種或商業品種DNA-PCR鑑定分析等相關資料，以對此產業的發展提供較為深入、充足及直接可用的相關訊息。並對品種育種權利的保護及侵權鑑定提供即時有利證據。(二) 使本場從事品種檢定相關業務之人員能熟悉品種檢定相關技術及品種保護制度面之了解，並進而提昇品種檢定及鑑定之技術水準。實際參與品種檢定之工作人員皆參加相關之技術講習會及參與聆聽專題演講。(三)透過分子標誌研究成果盤點，能確實掌握本相關核心技術與需進一步補強之技術缺口。配合國際上相關研究與應用分析資訊，將有助於我國相關研究之發展。而對以分子標誌進行侵權鑑定之流程，能有更明確之規劃與目標。 The projects of this program have three components. One of the components is to establish varieties identification through SSR molecular marker of new and/or commercial varieties of Phalaenopsis. The second is to training the faculty members to know the relative technologies of DUS test and system of plant variety protection through technique short-term training and keynote speech. The other one is to inventory count of molecular marker research that can help us to know the core technologies and reinforcement of the technical gap.The results of this program will provide helpfully informations of plantvariety protection.2011 植物種苗團隊-建立茄科重要性狀之分子標誌-番茄抗黃化捲葉病毒基因 The establishment of molecular markers platform by marker-assisted selection-Tomato yellow leaf curl virusTYLCV 100農科-1.1.10-種-X1 100AS-1.1.10-SS-X1 生物技術課 Biotechnology Section 孫永偉 Yung-Wei Sun 已建立Ty1-SCAR分子標誌，可擴增Ty-1抗病基因300 bp之DNA條帶，可擴增ty-1感病基因200 bp之DNA條帶。已建立Ty2-TYLCV-SCAR分子標誌，可擴增Ty-2抗病基因850bp之DNA條帶，可擴增ty-2感病基因750 bp之DNA條帶，可擴增TYLCV病毒基因550bp之DNA條帶。已建立Ty3-SCAR分子標誌，可擴增Ty-3抗病基因550 bp之DNA條帶，可擴增Ty-3感病基因250 bp之DNA條帶。上述分子標誌可協助育種者早期篩選抗病植株及確認抗病基因型(R/R、R/S、S/S)，提高育種效率。 In this study we designed primers of TYLCV Ty-1 Ty-2 Ty-3 genes. The results showed we has been established Ty1-SCAR molecular marker could be amplified resistance gene Ty-1 300 bp of DNA band could be amplified ty-1 susceptibility gene 200 bp of DNA bands. Established Ty2-TYLCV-SCAR molecular marker could be amplified Ty-2 resistance gene 850 bp of DNA band could be amplified ty-2 susceptibility gene 750 bp of DNA band andcould be amplified TYLCV virus gene 550 bp of DNA band. Established Ty3-SCAR molecular marker could be amplified resistance gene Ty-3 550 bp of DNA band could be amplified Ty-3 susceptibility gene 250 bp of DNA band.The molecular markers could help breeders early screening and confirmation disease resistant plant genotypes (R / R R / S S / S)2011 植物種苗團隊-植物種苗產業發展服務平台 Development and Service Platform forPlant Seed/Seedlings Industry 100農科-1.1.11-種-X1 100AS-1.1.11-SS-X1 繁殖技術課 Propagation Technology Section 李美娟 Mei-Jiuan Lee 基因轉殖木瓜multiplex PCR檢測技術與一般PCR檢測共累積715個檢測檢體資料，結果與原通過認證之檢測方式一致；於本年辦理基因轉殖大豆與玉米定性檢測訓練；認證實驗室輔導提供基因轉殖檢測監測小組成員TAF認證實驗室申請之資料與諮詢，並協助防檢局台中分局與禾鑫企業開發社等進行TAF測試實驗室認證申請，動植物防疫檢疫局台中分局於100年11月23日通過TAF認證。經網路搜尋盤點共13篇海芋病毒檢測技術論文，檢測對象以TuMV、DsMV為主，檢測方法以ELISA與RT-PCR較多，檢測技術研發單位為台大、興大、農試所、台中場、種苗場及美國農部。依據indirect-ELISA流程建立海芋病毒(TuMV、DsMV) 檢測作業標準1式。完成各廠牌病毒檢測標準品與試劑之品質查核，經本場申請，TAF於11月2日派員評鑑。完成標的使用者之需求訪查，多數使用者（個人）期望透過本產業服務資訊平台取得「產品資訊（含產品目錄）」（佔14.5%）、「品種資訊」（佔13.8%）及「專利資訊」（佔11.7%）等重要資訊；從組織/單位角度來看使用者期望之產業服務功能方面，多數使用者認為「品種資訊」（佔14.7%）、「技術服務」（佔11.8%）、「種子種苗相關產品供應」（佔11.8%）、「市場行銷」（佔10.3%）及「產業輔導資訊」（佔10.3%）是重要資訊服務，其分析結果可配合訪談進一步明確使用者之資訊服務需求。植物種苗繁殖技術盤點以專利文獻為主，學術文獻為輔之技術盤點分析手段，選擇植物種苗相關繁殖技術為研究標的，進行技術盤點分析。研究結果得知近三年國內外植物種苗相關繁殖之專利申請數量已逐漸降低，本研究推論該技術領域已逐漸成熟；而在學術研發能量上，1993年起政府開始投入大量研究經費支持植物種苗繁殖相關研究，此舉也帶動我國博碩士論文相關研究，相關研究也逐漸增加。從技術知識分析來看，全球植物種苗繁殖相關專利以新植物或獲得新植物之方法；藉由組織培養技術之植物再生為主要應用技術，佔35.9%。 The most common way of Genetically Modified (GM) or transgenic papaya inspecting method is Polymerase Chain Reaction (PCR) inspecting methodto amplify the sample target DNA fragment. We used the Multiplex PCR can reduce the testing costs. Held training for detected the GM soybean and maize with PCR. And we helped government units or company to apply the laboratory accreditation.13 papers related with calla lily viruses detection techniques were searched on websites and checked. The main detected targets are TuMV and DsMV. The most used techniques are ELISA and RT-PCR which are developed by NTU NCHU ARI TDAIS TSS and USDA. We have setted up the SOP of calla lily viruses (TuMV and DsMV) detection according to indirect-ELISA procedures. Also we have finished the quality checking of standards andreagents from 4 brands. After replying TAF has dispatched officials to estimate on Nov. 2.2011 植物種苗團隊-生物技術於植物品種開發及種苗驗證之應用研究-植物種苗認驗證體系建構 Construct the Accreditation system for plant seedlings 100農科-1.1.12-種-X1 100AS-1.1.12-SS-X1 生物技術課 Biotechnology Section 莊淑貞 Su-Jean Chuang 1.植物種苗產業發展服務平台－－標準檢測隔離設施建構：依據健康種苗檢測研發與基礎驗證之需求，進行隔離環境維修規劃並完成兩間隔離溫室自動門之維修，及克尼伯馬鈴薯G 1基本薯之生產，經檢驗為合格種薯共274.8kg。在G2種薯生產部分，已利用符合G2種薯生產標準之隔離網室（32目網室）1棟，完成G2種薯1400公斤之繁殖並通過驗證。另外依照防檢局100年9月7日1001485525號令修正發布之「馬鈴薯種薯病害驗證作業須知」標準，進行兩間G2繁殖圃隔離網室雙門的改善維護及完成種苗認驗證隔離溫室之高床製作及水牆、RO水供應系統運作。2.十字花科黑腐病及西瓜果斑病種子驗證體系建構:：參酌技術盤點之國內外文獻，以不同的樣品進行各種檢測方法的方法及靈敏度測試，目前已完成十字花科黑腐病及西瓜果斑病的標準檢測作業流程（SOP），並與國內相關機構學校合作，完成檢測能力試驗，建立符合TAF檢測實驗室認證文件各一式送交TAF認證基金會進行增項認證的審核工作。3.種子(苗)品質純度分子檢測技術研發及標準化：種苗公司自有4個小果番茄雜交品種之雜交組合(兩親本及雜交一代)為材料，100組ISSR通用引子中篩選出6組DNA標誌分別存在父本與F1或母本與F1，無法對F1材料單獨進行識別。利用SCAR方法對6組DNA標誌進行條帶回收、定序及專一性引子設計並對引子專一性進行確認。本計畫並進行Multiplex-PCR最佳反應條件建立，共開發1組Multiplex-PCR具條帶專一性的引子組，可同時對所收集的種苗公司自有4個小果番茄雜交品種之F1種子純度進行自交母本種子及父本種子之檢出。4. 種子健康檢查驗證體系之建立：為達到國際種子檢查協會(ISTA)要求各種子檢查室檢驗品質的精準度、整體性與一致性，本計畫依據ISTA發佈之國際種子檢查規則(International Rules for Seed Testing)，建立「種子健康檢查」品質手冊。並根據品質手冊規範制定「十字花科蔬菜種子黑腐病檢測」標準作業流程，進行行政管理與試驗技術過程並記錄表單，然後透過內部稽核達到品質與能力控管。本計畫除已完成「十字花科蔬菜種子黑腐病檢測」，之加項認證文件及檢測能力訓練等準備外，亦遵循ISTA檢查規則第7章之附錄(Seed Health Testing Methods)所列各病害檢測方法積極逐項建立標準作業流程以應將來業務運作時加項認證之須。(附錄中目前經ISTA認可之檢測方法共26項。) The purpose of this plan is to promote the plant pathogens detection and isolation efficiency of the green house.This year we promoted the function of the two plant pathogens detection and isolation green horses to fit in the certification requirements of the pathogens detection (The main detection methods of cruciferous black rot bacteria(Xanthomonas campestris pv. Campestris) and watermelon bacterial fruit blotch (Acidovorax avenae subsp. Citrulli)). These pathogens detected methods will be tested and then developed into a standardized screening process also accreditated by Taiwan Accreditation Foundation. One required index of high quality of seed/seedling is genetic purity. One of the objects of this program is through four directions to setup the control mode in seed/seedling industry by using molecular markers. Last object of this program is to set up the establishment conforms to the health testing international certification laboratory. To complete the seed testing quality control system instrument data. The information of accreditation of seed health testing has been collected from international Seed Testing Association (ISTA) National Seed Health System (NSHS) of USDA-APHIS and the International Seed Health Initiative (ISHI-Veg). That information will be used to complete the task of accreditation on seed health testing by Taiwan seed testing laboratory.2011 赴荷蘭研習植物品種保護技術 The study on technique used for plant variety protection by attending to Netherlands 100農科-4.1.1-種-X1 100AS-4.1.1-SS-X1 品種改良保護課 Plant Breeding Section 郭孄婷 Lan-Ting Kuo 本次研習主要在荷蘭Naktuinbouw進行。Naktuinbouw為荷蘭植物品種檢定之專責單位，除負責荷蘭國內之植物品種申請案件檢定之外，並負責歐盟申請案件之檢定。此次研習實際參與蝴蝶蘭、其他蘭花及其他觀賞植物等作物之品種檢定技術，包括實務操作、植物檢定方法之開發訂定及更新、資料庫建置等，並參訪實驗室部門了解DNA標誌在品種權上的應用。 The study was mainly done in Naktuinbouw Netherland. Naktuinbouw is the only authorized organization in the Netherlands which is responsible for DUS – testing of all varieties of ornamental vegetable and agricultural crops. And Naktuinbouw is not only responsible for domestic applications but also for applications in EU. This study included doing thedescriptions learning how to develop a test guideline and revise it and establishing database of varieties etc. The learning was especially involved in Phalaenopsis other orchids and other ornamentals. And the study also included visiting the Laboratories to learn about applications of DNA markers in plant variety right.2011 種子檢查技術研習 Study on seed testing technology 100農科-4.1.1-種-X2 100AS-4.1.1-SS-X2 種苗經營課 Seed & Seedling Management Section 許鐈云 Ciao-Yun Syu 今年度共有2員分別參加國際種子檢查協會(International Seed Testing Association，ISTA)研討會，其中一員參加品保制度研習於100年8月8~12日在印度 邦加羅爾舉辦，由Indo-American hybrid Seeds(India)種子公司承辦。研習由主席 Rasha El-Khadem博士主持，並與Ronald Don博士共同擔任本次研習講師。本次5日的研習包含品保制度簡介-顧客抱怨等25項課程及8月9日至編號IN07之ISTA認證實驗室進行實際操作，課程中並進行超過10次的小組討論與報告。另一員參加種子純度研習於9月27~29日於加拿大薩斯卡通(Saskatoon)舉行，由加拿大食品檢驗局(Canadian Food Inspection Agency，CFIA)承辦。研習內容包含： ISTA的目標、組織架構、檢查規則介紹； 「純潔種子定義( Pure Seed Definition PSD) 」探討；風選程序(Uniform Blowing Procedure)的使用、規範與校正；常見「其他種子(Other Seeds)」的鑑定與實習；純度分析相關植物學、品保制度及國際檢驗證核發之介紹等。研習結束後，參觀薩斯卡通CFIA種子實驗室的種子發芽與純度分析等業務，及前往渥太華拜訪CFIA植物病理實驗室的ISTA種子健康檢查與植物病害檢疫業務。 In this year two persons of TSS participated in different ISTA workshops individually. One attended the 2011 workshop on quality assurance in India which Indo-American hybrid Seeds(India) company held at Bangalore during August 8 to 12. The workshop was began by the Chairman of Dr.Rasha El-Khadem and co-chaired with Dr. Ronald Don lecturer in this study. The five-day study contained Introduction to quality assurance system - customer complaints and other 25 courses and more than 10 panel discussions and reports were include. At August 9 all of the Study participants went to No.IN07 of the ISTA laboratory for experimental operation and quality assurance exercises.The other attended the 2011 workshop on seed purity in Canada was held by Canadian Food Inspection Agency (CFIA) at Saskatoon during September 27th-29th. The content of this workshop was including the introduction of ISTA and seed testing rules pure seed definition (PSD) botany about seed purity analysis uniform blowing procedure calibration identification of other seeds2011 因應氣候變遷建立玉米採種體系 Setting up F1 seeds production system of feed corn for coping with climate change 100農科-4.2.1-種-X1 100AS-4.2.1-SS-X1 農場 Research Farm 陳學文 Hsueh-Wen Chen 不公開 Not public2011 優質番木瓜品種選育、採種及栽培技術改進 Good quality varieties selection and improvement the technology of cultivation and seed production on papaya 100農科-4.2.2-種-X1 100AS-4.2.2-SS-X1 屏東種苗中心 Pingtung Seed & Seeding Research Center 邱展台 邱展台 本年度持續進行引進之耐儲運黃肉品系之雜交第四代新選拔純化，選拔優良優良單株。另以果皮斑點少之品系為親本，進行雜交後代觀察。耐儲運品系雜交第4代，選拔出15個單株，其中有1個單株為黃肉，14個單株為紅肉，果實重量從363公克至1181克左右的小型至中型果，果實總可溶性物10-14 % Brix，果實品質優良，經雜交分離選拔後至F4已獲得紅肉耐儲運之小果品系。以Golden為親本之雜交一代的果皮生理性斑點仍相當多，有待進一步分離選拔。以幼年期及結果初期度過颱風多雨，以植株幼年期或結果初期，對疫病之抗性較強，在未特別增加農藥施用的情況下，植株及幼果未罹患疫病。 This year is ongoing selecting transportation- enduring character of fourth generation of the yellow strain crossed with mu1 strain. Use the skin flecks smaller lines as parents observation of the hybrids. Obtain 15 single plants from the 4th generation of transportation- enduring crossed with mu1 strain. There are 14 single plants possesses red flesh and 1 plant is yellow flesh fruit weight from 363 grams to around 1181 gramsof small to medium-sized fruit fruit total soluble solid 10-14%Brixwith excellent quality. F4 via hybrid separation and the selection of resistant storage and transportation the small fruits with red flesh has been obtained. Golden strain for the parents of the hybrids peel of physiological fleck is still a considerable number. It is need further separation and selection. Due to plant and fruit young stage have strongresistance to the blight planting seedlings in early summer plants and young fruits are not suffering from epidemic diseases.2011 植物種苗團隊-蔬菜品種改良及採種技術研究 Study on improvement of vegetable varities and technology of seed production 100農科-4.2.2-種-X2 100AS-4.2.2-SS-X2 品種改良保護課 Plant Breeding Section 郭宏遠 Horng-Yeuan Kuo 不公開 Not public2011 仙履蘭及熱帶球根花卉品種改良與種苗生產技術開發 Improvement on slipper orchid and tropic bulb flowers breeding and establishment the seedling productiontechnique 100農科-4.2.2-種-X3 100AS-4.2.2-SS-X3 品種改良保護課 Plant Breeding Section 劉明宗 Ming-Chung Liu 球根花卉品種選育在彩色海芋方面，94及95年雜交後裔中已選出具潛力單株共8株，進行單株性狀比較，結果以紫黑色系之品系95080具有花型佳之優點，可作為盆花品系，鵝黃色之單株29-2-(4)具花莖及花苞長之特色，但是花苞會有張開現象，需進行進一步評估與觀察。孤挺花方面，以具潛力9重瓣單株進行性狀比較觀察，其中白色系列以4X31-(1)表現最佳，為中小花系，屬於半重瓣，花梗長約38.2公分，具有種球小即可順利抽梗之特性。粉色系中以單株83X144之花色表現較佳，為花瓣中心呈粉紅色並向外暈白色，單株167X31為較特殊之重瓣品系，屬喇叭型重瓣，具有花型奇特之優勢。仙履蘭品種選育方面，依照仙履蘭育種四大目標進行雜交授粉工作，以Maudiae Type hybrids、標準型之交配種(complex Type hybrids)、多花與單花交配種、單花交配種(非complex及Maudiae Type)等四類雜交，本年度成功雜交共9組合。在雜交後裔篩選方面本年度共篩選出2個雜交組合之單株分別是PA94098及PA94116，具有花期長花型圓整、花梗長、栽培容易及生長速度快等優點。以孤挺花種苗二號-紅豔之周徑10~12公分種球為材料，進行開花球養成之肥培試驗，試驗結果顯示供試之不同施肥比例對於種球的增大上沒有顯著差異，而種球增大的速率以高頻率的施肥較佳。孤挺花種苗二號-紅豔之周徑13~14公分之生育，在定植後自第一片葉至第四片葉的抽出，平均所需時間依序分別約為12.3、24.6、28.0及35.5天，在完全生長葉片長度以第三、第四片葉表現較長，葉寬以第一、第二葉片較寬，定植60天之生育期間每粒小球平均可生長4.5±0.4片葉片。以BA及 Kinetin 50ppm、100 ppm及200 ppm浸泡金花石蒜雙鱗片10 min及40 min進行金花石蒜雙鱗片之繁殖，結果以BA藥劑處理100ppm浸泡10min小鱗球增生效果比較好，但經藥劑處理不同濃度及浸泡時間其效果並未比不經藥劑處理者佳，因此如能在蛭石中殺菌處理良好，即可達到良好的繁殖率。宮燈百合種球養成試驗，結果於黑籃中養成方面切球處理的子球重量為7.1g、次生球發生率為6.7%；未切球處理的子球重量為11.7g、次生球發生率為1.5%。在田間栽培方面未切球處理的子球重量為11.7g、次生球發生率為3.5%；切球處理的子球重量為7.5g、次生球發生率為6.7%。 Bulbous flowers in calla lilies breeding during in 2005 and 2006 hybrids we have selected potential progeny of ‘95080’ with dark-purple spathe and good horticultural characteristics. It can be used as potted flower. Another potential progeny of ‘29-2-(4)’ with bright yellow color spathe and long peduncle and spathe length but the spathe shape is not good .It needs to improve the spathe shape in the future.Bulbous flowers in amaryllis we have selected 9 potential hybrid progenies of doubleflower.The white line of ‘4X31-(1)’ the best performance of flower shape belonging to medium-small flower size pink color line of ‘83X 144’ is better performance for the center of pink petals and white shaded and ‘167X31’ line is unique of flower type. According to four goals in slipper orchids breeding we have selected 2 superior progenies‘PA94098’ and ‘PA94116’ with with a long flowering period round flowers long peduncle length easy cultivation and growth etc. For the cultivation test in amaryllis of TSS No.2-Red Splendor2011 花卉團隊-以化學性誘變法進行馬拉巴栗新品系選育 Chemical mutagens mutagenesis in Pachira macrocarpa 100農科-4.2.2-種-X4 100AS-4.2.2-SS-X4 品種改良保護課 Plant Breeding Section 郭孄婷 Lan-Ting Kuo 將採收之馬拉巴栗果子剖開，取出種子，以NAN3不同濃度進行浸種，分別為1mM、2mM及5mM，處理24hr、48hr後，將已開裂之種子與未開裂之種子分開種植，計算其發芽率並觀察誘變之結果，1mM、2mM及5mM NaN3處理24hr，已開裂種子之發芽率分別為50%、34.88%及22.2%，處理48hr後，所有處理之發芽率皆為0%。目前已有二株變異株，變異之性狀分別為葉脈中間具有淺色斑紋及節間短縮，葉形細長捲曲。組織培養建立方面，未成熟種子切段進行癒合組織誘導，可觀察到各段節於傷口處有癒合組織產生，以含24-D 1mg/L之培養基效果較佳，24-D 2mg/L之處理，其培殖體易褐化，其他處理則有子葉轉綠之情形，逆分化效果較輕微。所產生之癒合組織多發生於切口處，呈現鬆散狀，無觀察到擬胚的產生，需進一步觀察。 The seeds of Pachira macrocarpa were collected and treated with 1mM、2mM and 5mM NaN3 for 24hrs or 48hrs. After treatments the hygrochastic seeds were planted apart. Then counted the germination rates of seeds and observed if there was mutation. The germination rate of hygrachastic seeds treated with 1、2、5mM NaN3 for 24hrs wre 50% 34.88% and 22.2%.After 48hr the germination rate of all treated seeds were 0%. There have been two mutated plants induced by NaN3 treatment. One is with leaf variegation along the central vines and the other is with thinner and undulated leaves. As to callus induction medium with 1 24-D 1mg/L had the best effect on callus induction and 24-D 1mg/L caused browningfrequently. Under the other treatments2011 植物種苗團隊-植物新品種檢定技術之開發與執行 The Technical Development and DUS Test Execution of Plant Variety Protection System 100農科-4.2.3-種-X2 100AS-4.2.3-SS-X2 品種改良保護課 Plant Breeding Section 安志豪 Chih-Hao An 本年度已完成草擬仙客萊、觀音蓮及草擬修訂仙客萊品種性狀表與試驗檢定方法初稿。關於蝴蝶蘭及番茄等作物資料庫建立方面，已收集15個蝴蝶蘭及10個番茄商業品種並完成品種性狀調查及資料庫建置。本年度受理新品種性狀檢定工作計蝴蝶蘭與朵麗蝶蘭103件、文心蘭1件、蕙蘭2件及玫瑰8件；正進行性狀檢定中之案件為蝴蝶蘭與朵麗蝶蘭26件、文心蘭1件、蕙蘭1件及捧心蘭1件；檢定完成資料整理中為蝴蝶蘭與朵麗蝶蘭12件；已完成品種檢定報告為蝴蝶蘭與朵麗蝶蘭5件；檢定完成且審查結束為蝴蝶蘭與朵麗蝶蘭42件、文心蘭1件及石斛蘭1件。孤挺花色素分析經分析後橘色、紅色、粉紅及紫色系孤挺花花青素影響呈色有色素1與色素2，經由兩種色素含量比例的差異使花朵呈現不同顏色，不同品種間具有不同色素種類及含量之變化。 02011 優質植物種苗量產體系建立 Establishment of production system ofhigh quality seedlings 100農科-4.2.3-種-X3 100AS-4.2.3-SS-X3 繁殖技術課 Propagation Technology Section 林上湖 Shang-Hu Lin 於3月、6月、9月擷取燈秤花、苦藍盤頂芽及次節位之插穗，分別處理不同濃度IBA進行扦插試驗。苦藍盤之扦插較燈秤花易發根，且頂芽插穗有較佳之發根率，春（3月）、夏（6月）二季扦插發根率較秋季（9月）佳。燈秤花則以秋季扦插發根率較佳，不論插穗節位，皆具有較佳的發根率，但需要較長的發根時間。台灣欒樹與光臘樹種子發芽試驗中不論是泡水一天處理或亦直接播種者，在介質處理上，泥炭土與田土兩者在發芽率上並無差異。台灣欒樹種子發芽後128格穴盤苗在不同介質生育比較上，在泥炭土處理組之株高上比田土較高且有明顯的差異。台灣欒樹不論是24格穴盤苗亦或4.5吋膠袋苗，以泥炭土為介質者，各項生長表現亦優於田土。台灣欒樹4.5吋盆苗及24格穴盤苗移植至6吋塑膠盆後，以泥炭土為介質者不論在株高、葉片數、植株莖徑、大於10cm主根長數量及根重均比以田土為介質者表現較佳。在光臘樹部份，128格穴盤苗階段不同介質間，株高表現無顯著差異，但在葉片數上，田土高於泥炭土。在24格穴盤苗亦或4.5吋膠袋苗階段，則以田土為介質者，各項生長表現優於泥炭土。光蠟樹4.5吋膠袋苗與24格穴盤苗，移植至6吋塑膠盆後，以田土為介質者不管在株高、葉片數、植株莖徑、大於10cm主根長數量及根重上，均比泥炭土為介質者佳且有明顯差異。不同繼代次數馬鈴薯-克尼伯品種組織培養苗間植株生長及薯球產量在二代之間差異並不明顯。惟四代之間因種植時間受到不同環境因子之影響，薯球產量差異明顯。此外本試驗收集19個盆菊品種，檢測得知以Chrysanthemum virus B為菊花主要之病毒種類，約佔25%之危害率，無病毒株進行隔離並養成採穗株，於38/28℃之高溫處理比25/18℃影響植株葉片大小及節間長度，亦影響取穗品質。菊花施用必達肥30-10-10 可得較多的花朵數，以20-20-20及15-10-30比例可得較多的插穗數目。 The cuttings of shoot positions (Apical sub-apical) of Elaeagnus macrophylla Thunb. and Ficus formosana Maxim.that were taken cuttings and IBA treatment in March June and September. The result showed that the rooting rates of Ficus formosana Maxim. were easier than Elaeagnus macrophylla Thunb.The rooting rates of Apical shoot portions were easierthan sub-apical shoots of regardless of cutting collection seasoncutting position and IBA concentrations The rooting rates of Elaeagnus macrophylla Thunb. were high in September. But the higher rooting rates of Ficus formosana Maxim. need more time.In germination test of Taiwan goldenrain tree and Light sacrificial tree seeds the results of germination rates demonstrated that there was no significant difference between seeds accepted water soaking for one day and those with no soaking treatment whether seeding in soil or in peat moss mix. The averaged plant height of Taiwan goldenrain tree seedlings which germinated in 128-cell plug with peatmoss mix was significantlyhigher than the result with soil medium. The performance of Taiwan goldenrain tree seedlings grown in 24-cell plug with peat moss mix was better than those with soil medium. It was also the same in the result of seedlings grown in 4.5-inch plastic bags. After transplanting to 6-inch pots from 4.5-inch pots or 24-cell plug the performance of plantheight leaf number stem diameter the number of roots longer than 10 cm and root weight of plants grown in peat moss mix was better than those in soil medium. In the stage of 128-cell plug seedlings the leaf number of Light sacrificial tree seedlings grown in soil medium was more than those in peat moss mix. The averaged plant heights of both treatments were not significantly different. In the stage of 24-cell plug or 4.5-inch plastic bag seedlings2011 植物種苗團隊-亞太地區植物種苗產業研究與應用 Plant nursery research group- Studying and application on plant seedling industry in Asia- Pacific region 100農科-4.2.3-種-X4 100AS-4.2.3-SS-X4 技術服務室 Technical Service Section 周明燕 Ming-Yenn Chou 不公開 Not public2011 種子調製倉儲技術 Seed process and storage improvementtechniques 100農科-4.2.3-種-X5 100AS-4.2.3-SS-X5 種苗經營課 Seed & Seedling Management Section 洪建民 Chien-Min Hung 不公開 Not public2011 綠肥作物開發與利用 The exploitation of green manure crop 100農科-4.2.4-種-X1 100AS-4.2.4-SS-X1 種苗經營課 Seed & Seedling Management Section 鄭梨櫻 Lee-Ying Cheng 土壤是植物根群生長與發育重要的場所，土壤理化性好壞將影響植株生長與發育。本計畫為了解種植景觀綠肥對土壤肥力之影響，以作為休閒農場花海栽培管理模式之參考。本計畫調查結果，經過種植景觀綠肥的田區土壤容重較道路土壤容重為小；土壤孔隙度則為大。不同品種波斯菊以混合種波斯菊維護土壤理化性效益最佳。 Physical and chemical properties of soil affect plant root growth and development. The aim of the project is to understand the effect of landscape green manure corps on soil fertility. The project survey found that the volume weight is lowest in mix cosmosthe porosity is highest。 The volume weight is lowest in parking lot and trails.2011 有機團隊-有機種衣劑開發及種子苗管理機制之建立 Establishment of organic coating material and seedling management 100農科-4.2.5-種-X1 100AS-4.2.5-SS-X1 種苗經營課 Seed & Seedling Management Section 黃玉梅 Yu-Mei Huang 一、重要有機種子生產國制度研究1.本計畫位瞭解部前國內有機種苗供需雙方的現狀以及意見，進行問卷調查。經驗証有機農場部分發函件回收39件。種苗業者部分分發函件回收17件。2.整體而言，約83%的有機農家有在自行留種作業，沒有者17%。水稻秧苗自備者僅18%，向外面購買秧苗者82%；其中買到有機秧苗約36%，買不到有機秧苗71%有向驗證單位報告。有機農家外購雜糧種苗時60%有指定「有機種苗」。指定「有機種苗」，可買到的農家有42%，買不到的有58%。外購雜糧種苗時68%有指定「無農藥處理」。約44%的農家表示買到經藥劑處理的種子；其中有一半未向驗證單位報告。覆函的問卷中，就全部蔬菜種苗而言，自行採種留種的部分佔31%，與其他農友交換所得來的佔12%，外購種苗佔57%。外購種苗沒有一家能購買到有機種苗，而購買到的種苗中約三分之二沒有經過化學藥劑處理(41%)，約三分之一有經過藥劑處理(16%)。無法購到未經藥劑處理的蔬菜作物種子，只能買到經藥劑處理的種子時，有63%有事先向驗證單位報告，37%未事先報告。3.政府有機農法目前對種苗的規範，有48%的有農家認為合理。認為不合理者有52%。有23%認為政府應該強制規定須使用有機生產的種苗，若無法購買，則可以使用非有機生產，但絕對不可以經農藥處理的種苗。大體同前述的意見，但不得以主張可以用經農藥處理種苗者，有35.9%。有25.6%認為不需強制使用有機種苗，但絕對不可以經農藥處理的種苗。4.種苗業者僅6%有在國內販賣有機種苗，但有接受到詢問的業者有41%。未有出口有機種苗者，有接受到國外詢問的業者僅12%。有在進行採種工作的業者，並無一個在國內外進行有機採種，但有35%接到國內詢問，17%接到國外詢問。有意願嚐試在國內進行有機採種者有24%，在海外採種者有17%。若政府成立有機種苗資料庫，有65%業者願意提供資料。二、蔬菜種子有機種衣劑及添加物之開發以高嶺土與麥飯石及滑石粉與皂土依100:0、25:75、75:25及0:100等不同比例混合披衣於油菜種子，以100:0的高嶺土：麥飯石及0:100的高嶺土：麥飯石粉在紙上法皆為98%，砂床法為93%，田間萌芽率96%，與對照組無顯著差異。未披衣種子平均發芽日數為1.0天，顯著低於各處理，經披衣處理者，以100%高嶺土處理者為1.7天最低。以珍珠石粉為披衣基質添加機能性材質在十字花科種子披衣處理中，添加甲殼素及竹碳粉不影響披衣種子紙上法發芽率。添加不同機能性材質對油菜幼苗生育影響差異不顯著；在結球白菜上添加木徽菌、蘇力菌及甲殼素能提高其鮮重、乾重、莖長及莖徑；在甘藍上，經披衣處理者其幼苗生長皆較未披衣處理者高；花椰菜添加蘇力菌及苦茶粕對鮮重有顯著增加，乾重及莖長則各有高低，莖徑經披衣處理者皆較未披衣者高。各處理間對黑斑病之罹病效果不顯著。試驗中所用之添加物皆為市售商品化之產品，其濃度較高，未來朝向不同稀釋比例期能減少添加物對種子發芽影響。在茄科作物披衣處理試驗上，以高嶺土與滑石粉50:50之比例及SA在番茄及辣椒在各種發芽率表現上最為穩定，添加其他膠類雖表現對辣椒無明顯差異，來年將以及SA配方之底衣液為基礎，添加各機能性材質，期能對番茄幼苗生長有所助益。 In Taiwan about 83% of the certified organic farms experience seedsaving activities. Bur in organic rice producers only 18% grew their ownseedlings in their farm those purchased from outside only 36% acquiredorganic rice seedlings. Those purchased seeds of other field crops fromoutside only 25% acquired organic seeds. In organic vegetable production31% farms grew their own seeds 12% got seeds from other organic farm.There were 57% organic vegetable producers acquired seeds from seedcompanies none of them were organic seeds. Forty-eight percent organicfarmers were satisfied with current organic regulations on seedsrequirements. Twenty-three percent organic farmers favoured regulationsthat designate the use of organic seeds. Only 6% of seed/seedlingsuppliers sold organic seeds/seedlings; 41% suppliers received enquires of organic seeds domestically 17% received enquires from abroad. None of the investigated seed producers produced organic seeds. As high as 65% seed/seedling suppliers interested in joining a organic seeds database.By using the rate of 100:0 25:75 75:25 0:100 kaolinite and maifan stone talc and bentonite coated on the rape seed. The germination rate of pure kaolinite and maifan stone were 98% on top paper2011 作物種苗教育訓練效益評估之研究 Benefit appraisal of crops seedling technical training 100農科-5.2.1-種-X1 100AS-5.2.1-SS-X1 技術服務室 Technical Service Section 鍾依萍 I-Ping Chung 本計畫係針對種苗業者以及農民施以種苗技術之輔導訓練，並對本場辦理之農業技術訓練班在訓及結訓學員實施意見調查。在訓練輔導方面，本年度計辦理植物組織培養技術訓練班2梯次；蔬菜穴盤育苗技術訓練班1梯次、專題講座6場、發行種苗科技專訊四期。在訓學員訓練成效反應層次評估，非常好及好者達86.47%，學習層次評估上則由50分（100%）進步至72.75分（146%）。學員得知農業訓練相關訊息，以農政單位網站及當地農會為主要訊息來源，兩者佔70.26%。「朋友告知」亦佔有相當之比例（19.62%）。大部分結訓學員（84.81%）在受訓前無組織培養相關經驗。訓練後有23.42%之學員從事組織培養相關工作。有62.81%學員未來有意願投入，不會投入者10.74%，尚未決定者26.45%，亦佔相當之比例。最需要農政單位輔導的項目依序為生產技術、資金融通、財務管理、行銷管理、農地利用管理、人事管理、產銷組織等項目。目前從事農業工作中碰到並迫切需要解決的問題， 有達70.89%，（112人）的學員對「專業知識與技術」之需求，其次依序為土地、資金，產銷組織及行銷通路。 This plan is serves with counselling training the seedling technology in view of the seedling entrepreneur as well as the farmer and handles the opinion in .The student result to respond level extremely good and good reaches86.47% the study level appraised on progresses by 50(100%) score to 72.75(146%).The website of politics agricultural station and the local farmer association are the main sourses where student got the trainingmesseges both account for70.26 % . “ The friend informs ” also holds quite proportion(19.62%).The majority of the student (84.81%) are nontissue culture experience before the training but 23.42% students are engaged in the tissue culture work after the training . 62.81% students will future have the wish investment but 10.74%will not be still have 26.45% students are not yet make the decision. Most needs the projectwhich the agricultural politics unit counsels is production technology farmlandfundsmarketing2011 植物種苗團隊-提升植物種苗產業經營管理能力輔導策略研究 Enhance the ability of plant seed industry management counseling Strategy 100農科-5.2.1-種-X2 100AS-5.2.1-SS-X2 技術服務室 Technical Service Section 周明燕 Ming-Yenn Chou 植物種苗產業管理模式會因其在產業價值鏈所在位階差異而有不同需求。種子業需要更多的研發管理及行銷管理能量，以提升企業研發及產品行銷能力；育苗業較類似服務業型態，因此更需要品質管理及顧客管理觀念導入。組織培養業及蘭花生技業著重在量產及產品品質，產程管理及品質管控是最必要的能力。 Plant seedling industry management in the industry value chain due to their rank differences where there are different needs. Seed industry needs more energy R ＆ D management and marketing management to enhance research and development and product marketing capabilities; nursery industry than a similar service type and therefore need the concept of quality management and customer management to import. Tissue culture andorchid industry biotechnology industry focused on mass production and product quality production process management and quality control is the most necessary capacity.2011 植物種苗團隊-以知識密集服務為導向之種苗產業服務網站建置研究 Establishment of the Knowledge-Intensive Service Website for Seedling Industry 100農科-6.1.1-種-X1 100AS-6.1.1-SS-X1 技術服務室 Technical Service Section 蔡瑜卿 Yu-Ching Tsai 1.1-12月進行種苗網站資料維護603筆資料，辦理100年度種苗科技專訊4期之線上出刊作業。2.蒐集新增國內園藝景觀苗木業者、仙履蘭業者包含基本資料及主要產品、服務項目等資料143筆；台灣流通新品種資料107筆；植物繁殖技術相關文獻等3807筆等書目資料及電子全文。3.完成台灣園藝景觀苗圃產業概況分析報告一篇。4.辦理2場種苗業者座談會、4梯次提升種苗企業經營能力講習會及1場植物種苗科技研發成果研討會及海報展示，提供種苗業者及研究人員最新訊息與促進交流。調查參加種苗相關活動之種苗業者接受訊息管道，仍以書面紙本為主(68.4%)。5.不同類型種苗業對網路訊息傳遞應用情形調查：顯示種苗業產業類型不同，銷售對象與地區範圍不同，應用網路的情形有很大差異，以內需型且為銷售行為屬於地區性的種苗業類型網路應用的比例較低。6.種苗產銷資訊平台建立意願調查：以景觀苗圃業為調查對象，因其產銷資訊複雜不易取得，結果顯示83%業者願意加入資訊交流平台，多數業者對於景觀苗圃生產資訊的透明化皆持贊成態度，期望能增加曝光以及行銷之機會，但大部份需要協助整理資料上網。 The plan has compiled the information of seedling industry techniquesactivities and continued to check to evaluate to analyze in being system to investigate the opinion of users and to improve the function of website system. The plan provides the academia the industrial circles and the government departments with knowledge service about seedling industry.2011 建構亞太植物種苗產業服務資訊平台 Construction for Asia Pacific Seedlings Industry Services Platform 100農科-6.2.1-種-X1 100AS-6.2.1-SS-X1 技術服務室 Technical Service Section 蔡瑜卿 Yu-Ching Tsai 不公開 Not public2011 RFID及二維條碼應用於種子產銷管理之研究與開發 Study and development on RFID and QR code technology for the seed production and marketing management 100農科-6.3.1-種-X1 100AS-6.3.1-SS-X1 種苗經營課 Seed & Seedling Management Section 許鐈云 Ciao-Yun Syu 種苗場擁有9座種子冷藏庫，長年配合政府政策負責生產供應全省雜糧、綠肥及蔬菜作物種子，操作從契作生產及外購種子、調製、加工、倉儲、運輸至推廣銷售等一系列種子產銷流程作業。本年度針對種子倉儲部分建構「種子倉儲管理系統」部分，並結合無線射頻辨識(RFID)應用，達成種子倉儲流程作業電子化，除可供本場內部良好管理，增加種子倉儲管理透明性及即時性，此外結合RFID監控倉儲溫溼度環境情況，同時亦減少種子損耗，降低購買者損失。 Taiwan seed improvement and propagation station (TSS) has 9 seed refrigerated storages and is responsible for the seed production of cereal green manure and vegetable. Moreover there are a series of seed producing and marketing procedure operation including producingpurchasing processing packaging storage transportation and marketing. In this year it’s aim to accomplishment of the efficient method of seed storage management by RFID technology in order to maintain the seed of quality to decrease the waste of manpower and time and to enhance the check time of the inventory in the seed storage effectively.2011 作物遺傳種原收集、保存與利用 preservation collection and application of germplasm 100農科-8.1.1-種-X1 100AS-8.1.1-SS-X1 品種改良保護課 Plant Breeding Section 林正雄 Cheng-Hsiung Lin (一) 進行萵苣種原32個品種、十字花科蔬菜40個品種、瓜類30個品種等更新繁殖。(二) 持續台農種苗二號蜜雪梨之種原管理與保存。(三) 收集及保存本土綠美化種原20種，建立其繁殖技術及親本園。 1. Propagate and regenerate 100 vegetable varieties also investigate their properties.2. Continuing to keep and preservate the parents of Tainon No.2 pear.3. Collecting and preservating 20 varieties of local landscaping trees;developing its propagative technics and parent farm.2011 台灣香藥草植物資源開發利用 Development and utilization of indigenous aromatic herbs resources inTaiwan 100農科-8.4.1-種-X1 100AS-8.4.1-SS-X1 繁殖技術課 Propagation Technology Section 羅英妃 Ying-Fei Lo 不公開 Not public2011 作物種苗病害檢測、驗證及防治技術之開發與應用 Application and development of detection certification and control of crop diseases 100農科-9.2.1-種-X1 100AS-9.2.1-SS-X1 繁殖技術課 Propagation Technology Section 邱燕欣 Yen-Hsin Chiu a.作物抗病育種之病害檢定技術建立：完成番椒44品系167株果實接種炭疽病菌Colletotrichum acutatum，接種結果顯示有5品系植株病斑大小在4㎜以下，其他所有品系病斑均在4㎜以上；番椒29品系73株果實接種炭疽病菌Colletotrichum gloeosporioides，接種結果顯示有3品系植株不發病，有12品系植株病斑大小在4㎜以下，其他所有品系病斑均在4㎜以上；番椒9品系16株果實接種炭疽病菌Colletotrichum capsici，接種結果顯示有1品系植株不發病，有4品系植株病斑大小在4㎜以下，其他所有品系病斑均在4㎜以上。有抗性之植株種子將進行下一世代之選種。完成20瓶海芋瓶苗之POTY、CMV、TuMV、DsMV、ZaMV、ZaMMV、DsMV病毒檢測。完成馬鈴薯12瓶瓶苗之PLRV、PVS、PVX、PVY病毒檢測。完成61個馬鈴薯臺農一號G1種薯之PLRV、PVS、PVX、PVY病毒檢測。b.無病原種子種苗生產、處理及驗證技術之研究：以20株根圈微生物澆灌西瓜幼苗，評估微生物對西瓜幼苗生長之影響，發現微生物澆灌對西瓜幼苗株高有些微抑制；但其中12株對西瓜幼苗鮮重及乾重有促進的作用，可較對照組增加50%的重量。其中2支微生物(14-5及14-9)澆灌可以有效抑制西瓜幼苗果斑病罹病度。經試驗微生物對種子污染的西瓜幼苗果斑病防治效果較差，僅2支微生物(14-12、15-24)處理可有50%植株存活率，未來可進一步探討微生物與植物交互作用機制，分析其拮抗物質，以研製病害防治用生物製劑。c.抗病毒血清製備技術之開發與利用：完成蘭花病毒CymMV、ORSV 、CaCV鞘蛋白基因選殖，經誘導表現及免疫轉漬，確認所生產蛋白。進行蘭花病毒鞘蛋白大量生產及純化，送廠商進行動物(紐西蘭兔)免疫。未來所獲得的抗血清將應用於蘭花病毒檢測工作上。d. 作物病蟲害防治用 藥調查、研析及其 合理化應用技術開 發、改進：茄子栽培期間以窄域油500倍、苦棟油500倍、窄域油500倍+苦棟油500倍進行防治處理，並以不噴施藥劑做為對照，調查不同藥劑處理對茄子蟲害防治的效果，結果顯示上述藥劑處理對浮塵子、薊馬、銀葉粉蝨之防治效果並不明顯，對葉蟎雖然具短期防治效果，但效果亦不理想。在疫病防冶調查結果，使用亞磷酸500倍與1000倍溶液噴施4次後皆具有減緩或抑制疫病發生的效果，而亞磷酸溶液500倍處理對疫病的防治效果則較亞磷酸溶液1000倍處理效果為佳。 a.b. The influence on watermelon seedlings growth of 20 rhizobacteria were evaluated by watering the seedlings with overnight culture. And it is showed that by watering rhizobacteria the length of seedlings were suppressed a little. But 12 rhizobacteria have promoted the fresh weight and dry weight of the seedlings by 50% comparing with the untreated control. Among them 2 rhizobacteria (14-5 14-9)have suppressed bacteria blotch disease on watermelon seedlings effectively. After testing we discovered most rhizobacteria could not cure the pathogen-polluted seedlings. Only rhizobacteria No. 14-12 and 15-24 made 50% seedlings surviving.c. Three coat protein genes of orchid viruses-CymMV、ORSV 、CaCV have been cloned and confirmed by induced expression and wetern blotting with antivirus serum. After mass production and purification these virus coat proteins have been sent to immune rabbits.d. The eggplant treated with the narrow range oil(2㏄/l) China-berry oil(2㏄/l) narrow range oil(2㏄/l) plus China-berry(2㏄/l) investigated the effect for the insect pest of eggplant during cultivated period.Result showed that the effect of these treatments were not good for Leafhopper Thrips2011 花卉團隊-生產蕙蘭無特定病毒之種苗檢測系統之建立 The establishment of detection system of Cymbidium Specific-Pathogen-Freeseedling 100農科-9.2.1-種-X2 100AS-9.2.1-SS-X2 繁殖技術課 Propagation Technology Section 邱燕欣 Yen-Hsin Chiu 本計畫擬建立蕙蘭生產繁殖流程內病毒管控之檢測點 以協助農民避免生產流程可能造成之病毒感染影響產品價值。使用ELISA技術進行蕙蘭病毒檢測 檢測病毒包括ORSV與CymMV。檢測對象為分株苗之蕙蘭母本 結果顯示田間病毒普遍農民必須慎選種苗來源.為因應未來蕙蘭組培模式並依照組培模式擬定一檢測系統以供參考。 By this plan we try to establish the virus disease detection and management of Cymbidium production proceed. To avoid the value of Cymbidium Orchids been lower and lower cause by the virus infection.The ELISA was used as the detection method. And we also try to establish one virus detection and management model which is suitable for Cymbidium orchid tissue culture propagation system.2011 植物種苗團隊-符合外銷國之種子苗驗證技術研發、制度研究與建置 Establishment and Research of Seed and Seedling Certification Techniques and Regulations 100農科-9.2.1-種-X3 100AS-9.2.1-SS-X3 繁殖技術課 Propagation Technology Section 袁雅芬 Ya-fen Yuan 為協助業者將優質植物種苗（子）行銷國外市場，提升農產業競爭力，本研究規劃一完整且即時能提供各國植物種苗（子）檢疫資料庫，蒐集相關法規，共計836條條文，期能縮短資料檢索時間。文獻搜尋並比較國際試驗室間檢測番茄嵌紋病毒(Tomato Mosaic Virus，ToMV)的檢測方式保括ELISA、RT-PCR、ICRT-PCR等檢測技術，本計畫前期合成針對ToMV專一之引子對以及植體之Internal control primer set 可建立RT-PCR偵測受檢檢體內之ToMV，確定檢測之正確率。研究發現H2O2及乾熱處理對黑腐病污染及田間收取之種子皆有良好的減菌效果，藉由處理條件之最佳化調整，可有效殺滅表面攜帶之菌類。苦瓜種子發芽最適合之處理為60℃溫湯浸種10分鐘，發芽率可達80 %以上，沙床法與捲紙法可提高發芽率至90%以上。建立胜肽親和性篩選系統(peptides c7c peptides 7 peptides 12)對於標地蛋白之親和性汰選技術，建立及生產與ORSV病毒鞘蛋白具親和性之噬菌體，篩選出可利用於ELIS檢測ORSV之噬菌體。 For the purposes to help entrepreneurs marketing high grade crop seeds and seedlings overseas and upgrading competitive power of agricultural industries we have mapped out a complete database collecting 836 articles in total which provides various countries’ quarantine regulations immediately. Hopefully this can reduce time for data searching.By papers literature research we selected the RT-PCR as the major tech as our TAF testing method for Tomato Mosaic Virus. At the first we design a set of RT-PCR primer for ToMV specific primer set. We also design the internal control primer set for more reliable detection result.The protocol of H2O2 treatment followed by heating dry for killing Xanthomonas campestris pv. campestris (XCC) on cabbage seeds were evaluated to show better effects. A more précised H2O2 treatment will also be a pursuit.Bitter gourd seeds immersed in ℃ water for 10 min the germination rate could be reached above 80 %. The seeds at the sand bad or the paper roll were increased the germination rate above 90 %.By phage display systemthere were three kinds of phage library being selected by affinity panning selection including phage c7c library phage 7 library and phage 12 library. In phage c7c system2010 植物種苗團隊-作物特定性狀分子標誌建立及選殖 The establishment and application of specific molecular markers for the crops species 99農科-1.1.1-種-X1 99AS-1.1.1-SS-X1 生物技術課 Biotechnology Section 莊淑貞 Su-Jean Chuang 不公開 Not public2010 植物種苗團隊-作物種苗微體繁殖技術之開發與改進 The study of healthy seedling production by tissue culture 99農科-1.1.1-種-X2 99AS-1.1.1-SS-X2 繁殖技術課 Propagation Technology Section 文紀鑾 Chi-Luan Wen 不公開 Not public2010 植物種苗團隊-仙履蘭重要病害之分子監測及防治管理 Establishment of disease detection prevention and cure method in slipper orchids 99農科-1.1.5-種-X1 99AS-1.1.5-SS-X1 生物技術課 Biotechnology Section 張珈錡 Jia-Ci Chang 為獲得穩定的仙履蘭炭疽病菌(C. gloeosporioides)的接種源，已合成一個能有效促進產胞的培養基。針對仙履蘭炭疽病菌對蘭科或非蘭科寄主的專一性，得知該菌可為害蝴蝶蘭、嘉得利亞蘭、國蘭及文心蘭等蘭科植物，但不會為害非蘭科的作物。評估仙履蘭四品種對炭疽病的抗感性，結果顯示四品種均可被此病原感染，其中以iveum x sib和Hung Sheng Glow x Incantation雜交後裔最為感病。測試七種精油對仙履蘭炭疽病菌生長之影響，結果顯示草果與冬青精油抑菌的效果最佳。網室評估三種不同精油配方防治炭疽病菌的效果，結果顯示施用AF1-99配方配合撲克拉錳(6000倍)的防治的效果比單獨施用AF1-99配方或撲克拉錳的防治效果較佳。網室評估四支木黴菌防治炭疽病菌的效果，結果顯示T. viride與 T. harzianum兩支木黴菌可有效降低炭疽病的發生率。為達到早期病害檢測之目標，本試驗針對仙履蘭炭疽病菌設計專一性引子對90F/328R：(3'-GAC TTT GAA ATG CGA CTA CAG-5')/ (3'- GCC TTG CGA CGG AAC ATG GCG-5')可專一性偵測仙履蘭炭疽病菌，於300 bp可擴增一特異性條帶。進一步利用孢子懸浮液、菌株以及健康植株與菌株DNA不同混合比例，測試此專一性引子對之靈敏度。結果指出，於孢子濃度約100顆孢子/μl，或菌株DNA濃度約1 ng/μl，或植菌比為50：1，皆可有效檢測出仙履蘭炭疽病菌。 In order to obtain a stable inocula of Colletotrichum gloeosporioides a medium was formulated for enhancing sporulation of C. gloeosporioides. Orchid or nonorchid plants were inoculated to etermine host specificity of C. gloeosporioides. C.gloeosporioides was mainly pathogenic on Phalaenopsis Cattleya Cymbidium and Oncidium orchids. Four cultivars of Paphiopedilum were evaluated for their response to C . gloeosporioides. Among four cultivars tested two hybrid descendants niveum x siband and Hung Sheng Glow x Incantation were the most susceptible. Seven essential oils were investigated for their antifungal activities on C. gloeosporioides. Essential oils obtained from Amomum tsao-ko and Ilex were the most effective for suppressing mycelial growth of C. gloeosporioides. Three essential oil formulations were studied their antifungal activities on C.gloeosporioides in greenhouse. Results showed combination of AF1-99 oil formula and prochloraz manganese (6000 times) was the most effective for controlling the incidence of anthracnose disease compared with AF1-99 formula alone or prochloraz manganese (6000 times) alone. In greenhouse experiment four isolates of Trichoderma were tested for their abilities for control of anthracnose.Among four Trichoderma isolates tested T. viride and T. harzianum effectively reduced the incidence of anthracnose disease. A set of specific primers 90F/328R:(3’-GAC TTT GAA ATG CGA CTA CAG-5’)/ (3’- GCC TTG CGA CGG AAC ATG GCG -5’) was desiged to amplify a portion of the β-tubulin gene for the detection of C. gloeosporioides from slipper orchid. Results showed that a DNA fragment of about 300 bp was only amplified in slipper orchid2010 植物種苗團隊-基因轉殖作物種苗檢測監測體系之建立 Establishment of detection and monitors system for genetically modified seed 99農科-1.1.6-種-X1 99AS-1.1.6-SS-X1 生物技術課 Biotechnology Section 沈翰祖 Han-Tsu Shen 已蒐集基因轉殖玉米種苗Bt11、E176、GA21、MON810、NK603、MON863、TC1507、T25等8個品系，並構築PCR檢測片段至核酸載體為標準樣品。基因轉殖大豆Roundup Ready 品系參考樣品，並建立標準檢測流程，。執行基因轉殖作物種苗TAF檢測實驗室已完成本年度監督評鑑，並進行實驗室電腦條碼管理系統套裝化測試，且完成二次認證實驗室附加稽核、一次認證實驗室內部稽核與一次認證實驗室年度管理審查會議。並配合農糧署辦理二場基因轉殖作物檢測討論會。 The increasing presence of transgenic plant in the international markets has provoked a strong demand for appropriate detection methods to evaluate the existence of transgenic plants. The major objective of this study was to establish a detection technique for the GM maize soy and broccoli. The events of GM maize are Bt11 E176 GA21 MON810 NK603MON863 TC1507 T25 etc. The event of GM soybean is Roundup Ready Soy.For this purpose some screening methods based on target gene and select marker was development. The results showed that the GM crops could be detected through PCR-based markers. We constructed the reference materials of GM maize with the PCR products. And we prepared the reference materials of GM soybean with seed powder. The fluorescent detector could be detected the nptII gene in 1 minute.2010 植物種苗團隊-種苗生技廠商企業化經營輔導 Guiding agro-biotech manufactor of phalaenopsis for mass production of healthy seedling 99農科-1.1.8-種-X1 99AS-1.1.8-SS-X1 生物技術課 Biotechnology Section 廖玉珠 Yu-Ju Liao 不公開 Not public2010 植物種苗團隊-植物品種檢定流程分析、人員訓練及分子標誌開發 plant seed/seedling team – Analyzing the examining process flow training faculty members and developing molecular marker of plant variety right 99農科-1.1.9-種-X1 99 AS -1.1.9- SS -X1 生物技術課 Biotechnology Section 張惠如 Hui-Ju Chang (a)重要蘭科花卉品種及球根花卉品種分子標誌技術開發：將所設計86組SCAR引子，篩選30種紅花系商業品種(受品種權保護或其對照之品種)。目前10個引子組成六個引子對組合，可產生不同的差異性條帶，進一步將差異性條帶的有無給予0或1的編碼。經過識別編碼的組合而可區分紅花30個蝴蝶蘭商業品種。未來將擴大試驗材料族群，依花色粗分成四組，進一步進行SSR引子對之篩選工作。由種苗場種原圃取得31個品種(系)參試材料，包括花色為桔色系的14個品種(系)(含種苗二號-香吉士)，及紅色系的品種(系)(含種苗一號-桃姬)的17個品種(系)共31個品種(系)。DNA-PCR分析運用於親緣相似性分析，桔色花系或紅色花系單獨或合併分析，均有少數品種(系)無法由篩選出的32條再現性及穩定性高之分子標誌作完全區分。(a-1)委託嘉義大學生物農業科技系張助理教授岳隆針對蝴蝶蘭（Phalaenopsis spp.）品種分子標誌技術開發。本年度收集商業品種及雜交品系共52種，以AFLP及SCAR方法進行篩選品種間差異性。(b)檢定人員訓練：已於99年4月29日於中興大學舉辦「植物品種檢定流程及品種資料庫建制」之教育訓練，主要參與對象為農糧署、農業試驗所、林業試驗所、各農業改良場及學校機關等之實際參與植物品種檢定之人員，共有70人參與。另與中華種苗學會合辦於99年10月20日在種苗改良繁殖場舉辦「作物新品種檢定講習會」，講授者將執行過程及經驗與參與植物檢定人員分享，彼此熱烈討論。此次參與講習會之成員共有80人，對於作物新品種檢定技術有顯著提昇效果。(C)建置植物品種權檢定及鑑定整合平台：已完成品種權整合服務平台系統開發，並於「植物品種檢定流程及品種資料庫建制」之教育訓練，開放行政管理人員、技術管理人員及技術檢定人員上線操作。完成品種權整合服務平台系統雙軌試測，預定於12/10正式上線服務。另於性狀檢定資料庫內108項作物檢定性狀表建置工作，之後將透過檢定人員講習會，持續推動檢定人員將各作物之性狀資料建置上網，擴增資料庫內容。建立蝴蝶蘭影像取樣標準作業流程，初步建置蝴蝶蘭屬花朵性狀影像之色彩特徵擷取與資料庫平台。(d)分子標誌運用於育種、品種保護、品種純度與基因轉植作物安全管理之技術盤點與產業應用研究：本研究針對具外銷市場發展潛力與目前重要輸出之作物品項，將研究資料區分成三大項：輔助育種〈茄科、葫蘆科、十字花科〉、品種鑑定與分析〈毛豆與蘭科〉、純度鑑定等，其中在輔助育種項目中再細分成生理指標及病害兩細項，進行收集分析。目前已完成輔助育種：24篇、品種鑑定：14篇、純度鑑定：17篇等，共55篇資料整理分析。 The projects of this program have four components. One of the fourcomponents is to establish varieties identification through molecularmarker of new and/or commercial varieties of Phalaenopsis and calla lily.The second is to training the faculty members to know the relativetechnologies of DUS test and system of plant variety protection throughtechnique short-term training and keynote speech. The other one is toinventory supporting information system of character examine managementof plant breed right and plant variety identification of “Plant Varietyand Seed Law” for buildup the platform of infringement service. The lastone is knowledge management of molecular markers were used in breedingvariety protection and purity test.2010 植物種苗團隊-利用分子標誌輔助育種技術平台 The establishment of technique platform by marker-assisted selection 99農科-1.1.10-種-X1 99AS-1.1.10-SS-X1 生物技術課 Biotechnology Section 張惠如 Hui-Ju Chang 本研究係於文獻上及基因庫中發表之序列資料，設計合成偵測TYLCV病毒基因及Ty-1、Ty-2、Ty-3基因引子對，利用收集之試驗材料進行篩選。經過PCR試驗及電泳分析後，試驗結果顯示在目前的材料中，已建立偵測TYLCV與跟Ty-1、Ty-2、Ty-3基因連鎖之分子標誌各一組。另外，也將Ty-2及TYLCV之偵測引子對，進行建立Multiplex-PCR測試試驗，經試驗證實已可以在同一PCR條件反應下，同時偵測Ty-2及TYLCV。 The whitefly-transmitted Tomato yellow leaf curl virus (TYLCV) is major pathogen of tomatoes. In this study using the information from literature and published sequence databases design and synthesis primers.Based on our results the polymerase chain reaction-based markers were developed for detection of three major resistance genes of Tomato yellow leaf curl virus Ty-1/Ty-2/Ty-3 and a gene fragment of TYLCV. On theother hand the detection Ty-2 and TYLCV primer pairs were tested to establish Multiplex-PCR test. The result confirmed the reaction could be in the same PCR conditions to simultaneous detection of Ty-2 and TYLCV.2010 植物種苗團隊-植物種苗產業發展服務平台 Development and Service Platform for Plant Seed/Seedlings Industry 99農科-1.1.11-種-X1 99AS-1.1.11-SS -X1 繁殖技術課 Propagation Technology Section 張定霖 Ting-Lin Chang 不公開 Not public2010 植物種苗團隊-生物技術於植物品種開發及種苗驗證之應用研究-植物種苗認驗證體系建構 Construct the Accreditation system for plant seedlings 99農科-1.1.13-種-X1 99AS -1.1.13-SS -X1 生物技術課 Biotechnology Section 沈翰祖 Han-Tsu Shen 本計劃之執行在於提升病理檢測與隔離溫室效能，本年度計完成提升隔離溫室兩棟，可符合蘭花、馬鈴薯、豇豆…等病毒檢測TAF認證需求。一般基因轉殖大豆均利用PCR進行檢測，故依此建立基因轉殖大豆(Roundup Ready GTS 40-3-2)定性檢測標準作業流程，並於10月19日通過全國認證基金會(TAF) ISO/IEC 17025測試實驗室認證。十字花科細菌性黑腐病(Xanthomonas campestris pv. campestris)的檢測方法主要以選擇性培養基、SDS免疫擴散反應、ELISA、Biolog 快速鑑定系統為主，而瓜類細菌性果斑病（Acidovorax avenae subsp. citrulli）的檢測方式則以病徵診斷、組織鏡檢、病原菌分離鑑定、專一性引子對PCR反應、血清診斷及選擇性培養基為主。這些檢測方法經測試篩選後，將製訂為標準檢測流程，供未來診斷檢測之用。 The purpose of this plan is to promote the plant pathogens detection and isolation efficiency of the green house.This year we promoted the function of the two plant pathogens detection and isolation green horses to fit in the certification requirements of the virus detection (viruses which infect the crop include orchid potato and asparagus bean) of the Taiwan Accreditation Foundation. The most common way of Genetically Modified (GM) soybean inspecting method is Polymerase Chain Reaction (PCR) inspecting method to amplify the sample target DNA fragment.Establish the SOP of GM soybean (Roundup Ready GTS 40-3-2) qualitative detection method and passed the ISO / IEC 17025 accreditation. The main detection methods of cruciferous black rot bacteria (Xanthomonas campestris pv. campestris) are selective medium culturing SDS immunodiffusion ELISA and Biolog rapid identification system. For watermelon bacterial fruit blotch (Acidovorax avenae subsp. Citrulli)the detection methods are symptoms diagnosis tissue examinationpathogen isolation and identification2010 植物種苗團隊-赴荷蘭、法國研習植物品種保護技術 The study of technique for plant variety protection by attending to Nertherlands and France 99農科-4.1.1-種-X1 99AS -4.1.1-SS-X1 品種改良保護課 Plant Breeding Section 薛佑光 Yu-Kuang Hsueh 本次研習主要在荷蘭Naktuinbouw與法國CPVO進行。Naktuinbouw為荷蘭植物品種檢定之專責單位，除負責荷蘭國內之植物品種申請案件檢定之外，並負責歐盟申請案件之檢定。Naktuinbouw同時提供各項關於園藝作物繁殖材料品質的檢測服務和發證工作，具有法定效力，各項服務皆有收費標準，部分經費由政府負擔。此次研習實際參與蝴蝶蘭、其他蘭花、鬱金香、百合及甜椒等幾項重要植物之品種檢定技術，包括實務操作、植物檢定方法之開發訂定及更新等。也對品種之影像紀錄及資料庫查詢利用的架構及內容皆有認識及了解。並收集Naktuinbiuw之組織架構、業務、作業流程及品質管控之資訊，將有助於國內品種檢定經驗及水準之提升。不同國家品種權保護執行機構及管理各有不同，在歐盟各會員國除有獨立之執行機構外，並依歐洲植物品種權規範成立CPVO（Community Plant Variety Office 歐盟植物品種局），可在各會員國之間快速取得有效的品種權。本次參訪歐盟CPVO及法國Brion/GEVES品種研究機構，了解歐盟在申請新品種保護所需之審核及檢定過程，以及臺歐合作植物品種權蝴蝶蘭相互承認檢定報告書後，檢定案件之情形，藉此模式可加速蝴蝶蘭案件在歐盟審查之時程，並參訪法國國家品種檢定單位。 The study mainly was done on Naktuinbouw the Netherlands and CPVOFrance. Naktuinbouw is the only organisation in the Netherlands authorised to assess varieties of floricultural arboricultural and vegetable crops for distinctness uniformity and stability (DUS testing) for registration purposes and/or granting Plant Breeders' Rights and outside the EU is responsible for applications. Naktuinbouw on horticultural crops while providing the quality of reproductive material testing services and approve certifications with legal effect the service Jieyou charges funded in part by the Government. The study actually involved in Phalaenopsis and other orchids tulips lilies and sweet pepper varieties of plants such as verification of several important technologies2010 植物種苗團隊-基因轉殖、非基因轉殖及有機農業共存模式之建立 Establishment of GM and Non-GM crop coexistence model 99農科-4.1.1-種-X2 99AS-4.1.1-SS-X2 生物技術課 Biotechnology Section 張珈錡 Jia-Ci Chang 農委會國際處於本年3月25日安排加拿大農業部國際科技合作局周堅強副局長至種苗場參訪，並討論至加拿大進行基因轉殖作物檢測與生物安全等相關內容研習與參訪事宜。研習參訪單位為加拿大Agriculture and Agri-Food Canada ( AAFC)位於Saskatoon與Ottawa的研究中心、位於Ottawa的農業部與我國駐加拿大台北經濟文化代表處等，日期為9月11日至20日共10天，本次團員包括中興大學農藝系郭寶錚教授、台南區農業改良場楊藹華研究員、農試所鳳山分所李文立主任、種苗改良繁殖場沈翰祖副研究員等，並由團員共推郭寶錚教授為團長。目的與加拿大AAFC 研究人員進行基因轉殖作物檢測與生物安全科技交流。與加拿大農業部達成未來基因轉殖作物檢測與生物安全等科技人員互訪、我國派農業科系博士班或博士後研究生至加拿大進行相關科技研究等共識。我國駐加拿大台北經濟文化代表處科技組亦同意協助未來台加合作事宜。 For study the biosafety management of genetically modify crops in North America. So the COA combine the Taiwan Seed Improvement and Propagation Station Taiwan Agricultural Research Institute Tainan District Agricultural Research and Extension Station and college of agriculture and national resources of national Chung Hsing University tovisit the Agriculture and Agri-Food Canada ( AAFC) at Canada. The study project include the GM and Non-GM crops co-existence and the traceability and detection of GM crops2010 植物種苗團隊-推動亞太植物種苗交易中心與國際行銷策略研習 Plant nursery research group-The trainning for promoting Asia-Pacific plant nursery trading center and establishing international markrting policy 99農科-4.1.1-種-X3 99AS -4.1.1-SS-X3 繁殖技術課 Propagation Technology Section 李美娟 Mei-Jiuan Lee 期望以國際合作尋求荷蘭花卉拍賣市場將供需的資訊整合，以供我國建置建構亞太植物種苗網路資訊交易服務平臺之參考。希望透過此資訊整合的方式，以預約交易模式協助台灣種苗產業的銷售。 The aim of this project is to seek the help of the Netherlands to support the flower auction market supply and demand information by international cooperation for constructing the Asia-Pacific Network Information Plant Seed trading services platform in Taiwan. Through this information integrated approach to promote the reserved trading of Taiwan seeds and seedlings industry.2010 休耕田綠肥開發與利用 The exploitation of green manure crop for fallow field 99農科-4.2.1-種-X1 99AS -4.2.1-SS-X1 種苗經營課 Seed & Seedling Management Section 鄭梨櫻 Lee-Ying Cheng 一、綠肥種類與輪作模式對休耕田生態維護之效益評估為評估不同綠肥種類與輪作模式對休耕田生態維護之效益，應用4種綠肥於4項休耕田輪作模式並以全年休耕及一及二期皆種水稻為對照進行2年1輪試驗，調查各模式田區雜草量消長與病蟲害情形。99年進行第2年試驗，試驗結果，連續種植綠肥區之豆科綠肥有連作障礙現象，其中又以太陽麻最嚴重。各項綠肥以青皮豆及田菁可以前作綠肥自播方式進行一期作綠肥栽培。二、景觀綠肥油菊採種試驗為評估油菊於臺灣採種之可行性，本年度進行播種密度（每公頃15公斤、20公斤及25公斤）試驗。於9月播種，試驗結果各播種密度之開花期無明顯差異。 一、The evaluation of green manure crop for environment conservation on fallow field To evaluate the effects of green manure crop for environment conservation on fallow fieldthere are six different fallow system on paddy field were conducted and four green manure crops （Sesbania、Soybean、Sun hemp、Niger）were planted while field was fallow.The growth and decline of field were investigated.The results indicated that planting green manure or rice can repress weed growth and planting rice is more effective.Among the green manure cropsNiger had the best performance regarding the weed repression effect.二、Study of seed production of NigerThe aim of this study is to evaluate the possibility of seed production of Niger in Taiwan in order to solve the shortage of Niger seed due to unqualified seed（always mixed with weed seed）from overseas.This year we are going to decide the accurate planting density for seed production of Niger in Taiwan.2010 因應氣候變遷建立玉米採種體系 Setting up F1 seeds production system of feed corn for coping with climate change 99農科-4.2.1-種-X2 99AS-4.2.1-SS-X2 農場 Research Farm 陳學文 Hsueh-Wen Chen 不公開 Not public2010 優質番木瓜品種選育、採種及栽培技術改進 Good quality varieties selection and improvement the technology of cultivation and seed production on papaya 99農科-4.2.2-種-X1 99AS -4.2.2-SS-X1 屏東種苗中心 Pingtung Seed & Seeding Research Center 邱展台 Chan-Tai Chiu 不公開 Not public2010 植物種苗團隊-蔬菜品種改良及採種技術研究 Study on improvement of vegetable varieties and technology of seed production 99農科-4.2.2-種-X2 99AS-4.2.2-SS-X2 品種改良保護課 Plant Breeding Section 郭宏遠 Horng-Yeuan Kuo 不公開 Not public2010 花卉團隊-蘭花及球根花卉品種改良與種苗生產技術開發 Improvement on orchids and bulb flowers breeding and establishment the seedling production technique 99農科-4.2.2-種-X3 99AS-4.2.2-SS-X3 品種改良保護課 Plant Breeding Section 劉明宗 Ming-Chung Liu 球根花卉品種選育在彩色海芋方面，94及95年雜交後裔中已選出具潛力單株30株。孤挺花方面，從10個單株中獲得具潛力單株4株，分別是H911002、H912002、H922005及H921001，經量化繁殖比較試驗則以H911002繁殖倍率較高。蘭花品種選育方面，以蝴蝶蘭雜交後裔之小苗出瓶數量、死亡數、葉幅、葉片數等，作為初步篩選優良雜交組合之依據，共調查22個雜交組合，以P96057雜交組合最佳。在耐寒試驗篩選方面四個蝴蝶蘭雜交組合中。以P95040之雜交組合較具耐寒性。拖鞋蘭選育中，以短梗亞屬及多花亞屬當父本雜交各個不同亞屬種類當母本之拖鞋蘭雜交後裔比較，結果以P .bellatulum當父本，P. henryanum及P. liemianum當母本之平均葉片數及葉寬較多。在建立蕙蘭健康種苗驗證體系試驗方面，調查南投地區7家蕙蘭栽培業者，採樣12品種計119株，在嘉義地區調查5家業者，採樣6品種計46株，發現以錦旗、瑞梅跟大勳3品種之病毒發生率較高，為23.08%、50%及33.33%；其餘品種如玉花、太平洋、神童等，則無病毒檢出。利用培養基法測試七種精油對木黴菌與蝴蝶蘭黃葉病菌生長之影響，得知草果與冬青兩精油，可有效抑制蝴蝶蘭黃葉病菌的生長，但不會抑制木黴菌的生長。將上述具有抑菌的精油，依不同的比例混合成精油配方，然後進行培養基法測試不同經油配方對蝴蝶蘭黃葉病菌生長之影響，結果顯示AF1-99配方使蝴蝶蘭黃葉病菌的生長明顯受抑制。網室評估不同精油配方防治黃葉病菌的效果，結果顯示施用AF1-99配方配合撲克拉錳的防治的效果比單獨施用AF1-99配方或撲克拉錳的防治效果較佳。網室評估木黴菌防治黃葉病菌的效果，結果顯示木黴菌混拌至水草的防治的效果比覆蓋木黴菌的防治效果較佳。 We have selected 30 potential hybrid progenies during in 2005 and 2006 hybrid of calla lily and compared 4 potential hybrid progeniesincluding H911002 H912002 H922005 and H921001of amaryllis for bulk propagation. H911002 is the best for bulk propagation. We also have evaluated 22 hybrid progenies from the different horticultural characteristics in phalaenopsis. P96057 is the best hybrid combination.P .bellatulum as pollen parent and P. henryanum or P. liemianum as motherparent is the best combination for leaf numbers and leaf width in hybridprogenies.In order to establish the healthy propagation system we checked seven cymbidium’s farms in Nan-Tao about 119 plants including 12 varieties and 46 plants including 6 varieties in Chai-Yi. We found higher CymMV and ORSV virus infection in ‘Ching-Chi’，’Rei-Mei’ and ’Dai-Hsin’ varities.Seven essential oils were investigated for their antifungal activities on Fusarium solani and Trichoderma spp. Essential oils obtained from Amomum tsao-ko and Ilex suppressed more effective in mycelial growth of F.solani and less in that of Trichoderma spp. Essential oils with antifungal activities were used to develop different formulations to confirm their antifungal activity. AF1-99 formula (1000 times) significantly inhibited mycelial growth of F. solani. Two isolates of Trichoderma were examined for their sensitivity to prochloraz manganese (6000 times) fungicide. Results showed that the response of twoTrichoderma isolates grown on prochloraz manganese-amended culture media were resistant. Three essential oil formulations were studied their antifungal activities on C. gloeosporioides in greenhouse. Results showed combination of AF1-99 oil formula and prochloraz manganese (6000 times) was the most effective for controlling the incidence of anthracnose disease compared with AF1-99 formula alone or prochloraz manganese (6000times) alone. Mixing of Trichoderma inoculation into the sphagnum moss was effective for control of F. solani compared with Trichoderma mulch in greenhouse experiments. richoderma mulch had no negative effect on growth of root of Phalaenopsis whereas mix of Trichodema into the sphagnum moss did inhibit the growth of root.2010 植物種苗團隊-種子品質及種苗繁殖安全模式研發 Developed the seed treatment technologypromoting seed quality and seedlings cultivation in safe mode 99農科-4.2.2-種-X4 99AS-4.2.2-SS-X4 種苗經營課 Seed & Seedling Management Section 黃玉梅 Yu-Mei Huang 不公開 Not public2010 優質植物種苗量產體系建立 Establishment of production system ofhigh quality seedlings 99農科-4.2.2-種-X5 99AS-4.2.2-SS -X5 繁殖技術課 Propagation Technology Section 林上湖 Shang-Hu Lin "拖鞋蘭的商業雜交品種PA6466 (Maudiae type)、PA5909 (多花)及PA6394 (Complex type)，以不同稀釋倍率、不同頻率施用百得肥（15-20-25）之試驗當中，Maudiae type以2000x/2w之處理有較佳的效果，各生長量達42%以上，；多花雜交品種PA5909之葉片數以2000x/2w之施肥處理較佳，生長量可達79%，平均葉片數達8片；Complex type PA6394葉片數以2000x/2w之施肥處理較佳，生長量可達69.10%。在根系的生長發育上，以2000x的處理對根生長抑制較小 。拖鞋蘭小苗及中苗之植株間差異以葉面積差異最大，預計進一步分析C/N比，以了解各性狀及開花能力間是否具有相關性。蝴蝶蘭白花品種“V3”(Phal . Sogo Yukidian [P . Yukimai × P . Taisuco Kochdian]; 大花)、紅花“KHM0378”(Dtps. Sinica Sunday; 多花性; 大花)及“KHM1269”(多花性; 小花)三個品種為植物材料，以不同鹽類養液，分別為種繁No. 1、種繁No. 2、種繁No. 3、種繁No. 4、種繁No. 5及種繁No. 6進行栽培，並紀錄蝴蝶蘭之生長發育及介質pH值和EC值之變化情形。由試驗結果得知，蝴蝶蘭""KHM1269""品種3寸盆苗以種繁NO.6之營養生長狀況為佳，3.5寸盆苗則以種繁NO.2之營養生長狀況較佳；""KHM.378""品種3寸盆苗以種繁NO.2、NO.4之營養生長狀況為佳，3.5寸盆苗則以種繁NO.2之營養生長狀況較佳；而""V3""品種3寸盆苗之生長發育，則以種繁NO.2、NO.4之狀況為佳，，3.5寸盆苗則以種繁NO.2、NO.3之營養生長狀況較佳。豇豆-三尺青皮品種經過不同有益微生物處理後，植株成活率、地徑生長量、果莢重量及均重表現均以木黴菌處理組之表現最佳，但相對於萎凋病之致死侵襲，致提昇產量之效果並不穩定。而健旺誘導劑等處理(Messenger STS)對豇豆-三尺青皮品種均具有提昇植株生長及果夾產量之效果，但同樣無法排除萎凋病對整體型質及產量之影響。" "The plantlets of commercial hybrid of Paphiopedilum PA6466 (Maudiaetype)、PA5909 (multiflower) and PA6394 (Complex type) were fertilizedwith Peters (15-20-25)with different diluted folds (1000x 2000x)andfrequency(per week every 2week and every 4 weeks).The results showedthat 2000x/2w had the best effect on growth of PA6466 (more than 42%) PA5909 leaf number (up to 79% and the average leaf number 8) and PA6394(grew more than 69.1%). The growth of roots was also better when fertilized with Peters 2000x/2w. Comparing small plantlets (5 months-old) and medium plantlets (12-18 month-old) of Paphiopedilum Maudiae type and Complex type the leaf areas were the most different between two size.And have to determine the C/N ratio to associate with the florescenceability.The Phalaenopsis with white flower"" V3 "" (Phal. Sogo Yukidian [P. Yukimai × P. Taisuco Kochdian]; Big flower)、the Phalaenopsis with red flower "" KHM0378 "" (Dtps. Sinica Sunday; Flowery; Big flower) and "" KHM1269 "" (flowery ; Tiny flower) are plant materials by culturing the differently liquid salinely solution in order to Seed Propagation No.1、Seed Propagation No.2、Seed Propagation No.3、Seed Propagation No.4、Seed Propagation No.5 and Seed Propagation No.6. Record the growth and the change situations of medium pH value and EC value. Result of the experiment the Phalaenopsis "" KHM1269"" cultured in 3 inch pot with planting Seed Propagation No.3 was growing as the best state and in 3.5 inch pot with planting Seed Propagation No.3 was growing as the best state .the Phalaenopsis "" KHM1269"" cultured in 3 inch pot with planting Seed Propagation No.2、No.4was growing as the best state2010 台灣植物資源產業化應用 Application of industry of Taiwan vegetation resources 99農科-4.2.2-種-X6 99AS-4.2.2-SS-X6 繁殖技術課 Propagation Technology Section 林上湖 Shang-Hu Lin 於3月、6月、9月擷取大葉胡頹子、狹葉羊奶頭頂芽及次節位之插穗，分別處理不同濃度IBA進行扦插試驗；狹葉羊奶頭之扦插較大葉胡頹子易發根，且頂芽插穗有較佳之發根率，春（3月）、夏（6月）二季扦插發根率較秋季（9月）佳；大葉胡頹子則以秋季扦插發根率較佳，不論插穗節位，皆具有良好的發根率，其他季節為則以次節位插穗之發根率為高，因此，依據本試驗結果顯示，春、夏二季為狹葉羊奶頭、秋季為大葉胡頹子較佳之插穗擷取扦插之季節。長萼瞿麥於2月中旬進行種子播種及扦插繁殖時，可得到高發芽率且植株生長良好的基隆蠅子草種苗，為繁殖之最佳適期。利用3吋生物可分解塑料軟盆及相同規格之一般塑膠軟盆之128格櫻花穴盤苗換盆生育試驗結果顯示，溫室內可分解塑料軟盆在地徑、苗高、根重、莖重及葉重表現方面均優於一般塑膠軟盆。而露地試區二者之間差異則並不明顯。 The cuttings of shoot positions (Apical sub-apical) of Elaeagnus macrophylla Thunb. and Ficus formosana Maxim.that were taken cuttings and IBA treatment in March June and September. The result showed that the rooting rates of Ficus formosana Maxim. were easier than Elaeagnus macrophylla Thunb..The rooting rates of Apical shoot portions were easierthan sub-apical shoots. of regardless of cutting collection seasoncutting position and IBA concentrations The rooting rates of Elaeagnus macrophylla Thunb. were high in September. But the higher rooting rates of Ficus formosana Maxim. were obtained in March and June‘s cuttings.Dianthus superbus L. var. longcalycinus(Maxim) Will could get bettergermination rate and well-performance seedling by seed sowing and cutting in the mid March when is the best suitable propagation period.Use of 3 inch bioplastic containers and same size plastic containers to conduct survival and growth of 128 cell plug seedlings of Prunus campanulata test results showed that two kings of containers in stem diameter shoot height root weight leaf weight and stem weight2010 植物種苗團隊-植物新品種檢定技術之開發與執行 The Technical Development and DUS Test Execution of Plant Variety Protection System 99農科-4.2.2-種-X7 99AS-4.2.2-SS-X7 品種改良保護課 Plant Breeding Section 安志豪 Chih-Hao An 本年度已完成訂定夏堇和黛粉葉品種性狀表與試驗檢定方法及草擬仙客萊品種性狀表初稿。關於蝴蝶蘭、文心蘭及番茄等作物資料庫建立方面，已收集15個蝴蝶蘭、5個文心蘭及番茄商業品種並完成品種性狀調查及資料庫建置。本年度受理新品種性狀檢定工作計蝴蝶蘭與朵麗蝶蘭77件、文心蘭11件、捧心蘭1件及玫瑰3件；正進行性狀檢定中之案件為蝴蝶蘭與朵麗蝶蘭31件、文心蘭4件、石斛蘭1件及玫瑰15件；檢定完成資料整理中為蝴蝶蘭與朵麗蝶蘭15件；已完成品種檢定報告為蝴蝶蘭與朵麗蝶蘭10件、文心蘭2件；檢定完成且審查結束為蝴蝶蘭與朵麗蝶蘭38件、文心蘭4件及玫瑰6件。孤挺花色素分析經初步分析後橘色、紅色、粉紅及紫色系孤挺花花青素影響呈色有色素1與色素2，經由兩種色素含量比例的差異使花朵呈現不同顏色，類黃酮素有別於花青素，其常有共色作用而影響花色素表現，扮演了重要的輔色素作用。 The purpose of this project is to establish the test guidelines and execute DUS tests. We modified the test guideline and table of characteristics of torenia dieffenbachia and cyclamen. We also collected 15 commercial varieties of phalaenopsis and doritaenopsis and 10 commercial varieties of oncidium and tomato and investigated theircharacteristics for establishing variety database. We accepted 77 cases of phalaenopsis and doritaenopsis 11 cases of oncidium 1 cases of lycaste and 3 case of rose to execute DUS tests for protecting the breeder’s rights this year. 31 cases of phalaenopsis and doritaenopsis4 cases of oncidium 1case of dendrobium and 15 cases of rose have beenexecuting DUS tests. 10 cases of phalaenopsis and doritaenopsis and 2 cases of oncidium have been finished DUS reports. 38 phalaenopsis and doritaenopsis 4 oncidium and 6 roses have been granted to be new varieties. After Hippeasrum preliminary analysis pigment .The effect petals color of orange red2010 馬鈴薯健康種薯繁殖供應體系應用無線射頻辨識技術之研究 Studies on the Application Healthy Seed Potatoes Supply System by RFID Technology 99農科-6.1.3-種-X1 99AS-6.1.3-SS-X1 技術服務室 Technical Service Section 黃少鵬 Shao-Peng Huang 不公開 Not public2010 植物種苗團隊-亞太地區植物種苗產業研究與應用 Plant nursery research group-Studying and application on plant nursery industry in Asia-Pacific region 99農科-4.2.2-種-X8 99AS-4.2.2-SS-X8 技術服務室 Technical Service Section 李美娟 Mei-Jiuan Lee 1.探討亞太地區種苗產業現況及發展趨勢，盤點台灣植物種苗進入亞太地區市場利基所在。2.篩選臺灣有國際競爭潛力的物種和其在國際種苗分工上最有比較利益的環節。3.從全球植物種苗產業定位，規劃台灣成為亞太植物種苗網路資訊交易中心。 1. To explore the status and the development trends of plant seedling industry in the Asia Pacific region and inventory Taiwan niches for penetrating into the market of the Asia-Pacific region.2 . To screen the seedlings item of Taiwan with potential for international market competition and the comparative advantage part of plant seedling industry chain.3. To plan Taiwan to be the Asia Pacific Network Information Exchange Center of plant seedlings.2010 建立有機栽培之種子苗生產管理機制 Study on the management mechanism for organic seeds and seedlings growing 99農科-4.2.2-種-X9 99AS -4.2.2-SS-X9 技術服務室 Technical Service Section 周明燕 Ming-Yenn Chou 不公開 Not public2010 種子調製倉儲技術與園藝設施管理研究 The study of seed process/storage techniques and horticulture facilities management 99農科-4.2.3-種-X1 99AS-4.2.3-SS-X1 種苗經營課 Seed & Seedling Management Section 鍾伊婷 I- Ting Chung (一)、以人工手採之成熟大波斯菊(Cosmos bipinnatus Cav.)種子為對照組，比較機械採收處理(1)先機械脫粒後乾燥機乾燥及(2)先乾燥後機械脫粒等二種處理對於種子發芽率之影響。結果顯示，人工採收之成熟種子的發芽率較機械採收處理者高，為87%，具顯著差異。機械脫粒及乾燥之調製次序變動對大波斯菊種子發芽率之影響不顯著。(二)、油菊[Guizotia abyssinica (L. f.) Cass.] 整株採收後，以人工手採成熟種子為對照組；機械採收處理分為(1)先乾燥後脫粒及(2)先脫粒後乾燥二種方式。結果顯示，先乾燥後脫粒處理之發芽率與人工採收之成熟種子的發芽率達90%以上，與先脫粒後乾燥處理之發芽率80.2%，具顯著差異。利用脫穀筒進行機械脫粒之種子發芽率仍可維持80%以上，且較傳統人工脫粒節省人力成本。(三)、於番茄 (Lycopersion esculentum Mill.)“花蓮亞蔬18號”植株始花後，以硫酸銨、磷酸鈣、氯化鉀或硫酸鉀，氮磷鉀比20:20:20之肥料進行試驗。隔週施用一次推薦用量(12 g /per plant )及1.5倍推薦用量(18 g /per plant )之肥料，比較二種鉀肥及二種肥料用量對大果番茄 “花蓮亞蔬18號”果重、錘度、酸度及糖酸比之影響。試驗結果顯示，施用肥料濃度增加，使果重及酸度下降，錘度及糖酸比提高。 1. Garden cosmos (Cosmos bipinnatus Cav.) seeds mature at different times. Control is the mature seeds harvested by workers dried by sunshine treatments are (1) seeds were dried by drying machine first and then threshed by thresher; (2) seeds were threshed by thresher first and then dried by drying machine. The seed germination rate of control is 87% and there are non-significant between two treatments.2.Niger [Guizotia abyssinica (L. f.) Cass.] seeds mature at different times. Control is the mature seeds harvested by workers dried by sunshine treatments are (1) seeds were dried by drying machine first and then threshed by thresher; (2) seeds were threshed by thresher first and then dried by drying machine. The seed germination rate between controland treatment (1) are higher than 90% and there are significant between treatment (1) and (2).3. Treating the tomato (Lycopersion esculentum Mill.)“Hualien-Yasu No.18” with two levels (12 g/plant or 18 g/plant) of fertilizers and two kinds of K-fertilizers (KCl or K2SO4) once every two weeks in the beginning of florescence. By increasing the fertilizers the fruits weight and acidity decreased but the soluble solid and soluble solid/acidity ratio increased.2010 植物種苗團隊-發展安全作物種苗相關措施之整合研究 Research on the merger of managementprinciples for seeds and seedlings industry development 99農科-5.1.1-種-X1 99AS-5.1.1-SS-X1 技術服務室 Technical Service Section 周明燕 Ming-Yenn Chou 我國蔬菜種子生產業者85%擁有自有品種，顯見該項產業已在產業鏈中具有核心能力，但對於智財之保護則仍以市場策略及技術性保護為主，僅少數透過法律保護所擁有之智財。我國種子生產業者95%以上在國外設立有採種基地，搶攻國際市場，因此，種子生產技術乃確保競爭優勢的核心能力，也是業者迫切期待自我提升的重要能力；除此業者自身技術及經營管理能力提昇之外，公領域外在環境的配合，如提供病害檢測服務、非基因種子驗證證明、提供產業整合行銷平台，乃至資金、土地、行銷資訊之協助取得等將可作為業者強有力的後盾。 Our vegetable seed production companies have their own varieties of 85%showing the industry has been in the industry chain has core competencies but to the rules about intellectual property protection is still a market strategy and technical protection only a few are owned by legal protection of rules about intellectual property.Our seed production companies more than 95% have established abroad-basevying for the international market therefore the seed production technology is to ensure that the competitive advantage of core competence but also the industry urgently look forward to the important ability to elevate themselves; in addition to the companies ' own technical and managerial competence public areas of the external environment such as providing disease detection services validation of non-gene-seed2010 作物種苗知識傳播之執行效益評估 Benefit appraisal of crops seedling knowledge dissemination 99農科-5.2.1-種-X1 99AS-5.2.1-SS-X1 技術服務室 Technical Service Section 蔡瑜卿 Yu-Ching Tsai 本計畫係針對種苗業者以及農民施以種苗技術之輔導訓練，並對本場辦理之農業技術訓練班在訓及結訓學員實施意見調查。在訓練輔導方面，本年度計辦理植物組織培養技術訓練班4班、專題講座8場、發行種苗科技專訊四期。在訓學員訓練成效反應層次評估，非常好及好者達84.20%，學習層次評估上則由49.17分（100%）進步至72.50分（147%）。學員得知農業訓練相關訊息，以農政單位網站及當地農會為主要訊息來源，兩者佔70.90%。「朋友告知」亦佔有相當之比例（18.66%）。大部分結訓學員（83.58%）在受訓前無組織培養相關經驗。訓練後有26.12%之學員從事組織培養相關工作。有55.55%學員未來有意願投入，不會投入者12.12%，尚未決定者24.23%，亦佔相當之比例。最需要農政單位輔導的項目依序為生產技術、資金融通、行銷管理、財務管理、農地利用管理、人事管理、產銷組織等項目。未來從事農業工作中最迫切需要的資源時，有達80.60%，（108人）的學員對「專業知識與技術」之需求，其次依序為資金（70.15%，94人），土地、加入產銷班及行銷通路。 This plan is serves with counselling training the seedling technology in view of the seedling entrepreneur as well as the farmer and handles the opinion investigation.The student result to respond level appraisedextremely good and good reaches84.20% the study level appraised on progresses by 49.17(100%) score to 72.50(147%).The websiteOf politics agricultural station and the local farmer association are the main sourses where student got the training messeges both account for 70.90%.“ The friend informs ” also holds quite the proportion (18.66%).The majority of the student (83.58%) are non-tissue culture experience before the training but 26.12% students are engaged in the tissue culture work after the training . 55.55% students will future have the wish investment but 12.12%will not be still have 24.23% students are not yet make the decision. Most needs the project which the agricultural politics unit counsels is production technology fundsmarketingfinancial control2010 植物種苗團隊-植物育種研發成果移轉機制建構及媒合平台建置 Construction of a platform for matching and transfer of COA's plant breeding results 99農科-5.3.2-種-X1 99AS-5.3.2-SS-X1 品種改良保護課 Plant Breeding Section 郭宏遠 Horng-Yeuan Kuo 本計畫辦理「99年公部門蔬菜育種成果展示會」乙場與建置「植物育種半成品媒合平台及資料庫」，將增加公部門育成新品種或半成品之能見度與轉移成功之機會，加速品種育成速度與國內種苗業競爭力。 A exhibbition of vegetable breeding achievementsin public sectorsand a platform for transfering of semi-products of plant breeding were conducted this year. The main purposes of the plan are to increase opportunities showing the public sector’s vegetable breedingachievements speed up the cooperation between the public sectors and the industries in order to match and apply the breeding achievements and finally looking forward to promoting the competitiveness of the domestic seed industries effectively.2010 番茄種子披衣處理商品化技術研究(98-99產學合作) Study on seed coated treatments of tomato in commerical production 99農科-5.4.2-種-X1 99AS-5.4.2-SS-X1 種苗經營課 Seed & Seedling Management Section 黃玉梅 Yu-Mei Huang 不公開 Not public2010 草莓健康種苗產程管理技術開發(98-99產學合作) Healthy strawberry seedling productionand management for year -round 99農科-5.4.2-種-X2 99AS-5.4.2-SS-X2 繁殖技術課 Propagation Technology Section 文紀鑾 Chi-Luan Wen 不公開 Not public2010 植物種苗團隊-以知識密集服務為導向之種苗產業服務網站建置研究 Establishment of the knowledgeintensive service website for seedling industry 99農科-6.1.1-種-X1 99AS-6.1.1-SS-X1 技術服務室 Technical Service Section 蔡瑜卿 Yu-Ching Tsai 不公開 Not public2010 蔬菜專業育苗場經營及產苗安全預警資訊系統開發 Production Management and Development Security Production System For Vegetable Seedling 99農科-6.1.2-種-X1 99AS-6.1.2-SS-X1 技術服務室 Technical Service Section 黃少鵬 Shao-Peng Huang 不公開 Not publicPK XU`<<xl/workbook.xml PK X0kxl/theme/theme1.xml PK XUZZ xl/styles.xml PK X6[Content_Types].xml PK XJjLL _rels/.rels PK X?d--xl/_rels/workbook.xml.rels PK X֒|ZZdocProps/core.xmlPK Xw,,docProps/app.xmlPK X}f}fxl/worksheets/sheet1.xmlPK XU`<<jxl/workbook.xmlPK X0kkxl/theme/theme1.xmlPK XUZZ xl/styles.xmlPK X6[Content_Types].xmlPK XJjLL V_rels/.relsPK X?d--˘xl/_rels/workbook.xml.relsPK >0