1. data.coa.gov.tw
  2. 種苗研發成果
  3. 18831829
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年度 2013 植物種苗研究團隊-番茄抗病與花椰菜自交不親和性分子標誌建立與應用 The establishment and application of specific molecular markers for tomato and cabbage traits 102農科-9.1.1-種-X1 102AS -9.1.1-SS-X1 生物技術課 Biotechnology Section 孫永偉 Yung-Wei Sun a.番茄抗萎凋病基因型之分子鑑定：番茄萎凋病為重要真菌性病害，本試驗建立multiplex PCR (含I-2與Fusarium oxysporum)共顯性分子標誌(I2/Fu)，可同時檢定番茄抗萎凋病與病原菌基因。利用此分子標誌可擴增抗萎凋病基因700或852 bp之DNA條帶、擴增感病基因2320 bp之DNA條帶、擴增萎凋病(race2)基因1000 bp之DNA條帶。此分子標誌檢測結果與國內育種者或種子公司已知抗感性品種吻合。b.花椰菜自交不親和性基因型之分子鑑定：利用花椰菜常用之7個育種親本品系為試驗材料，以Brace等人在1994年建立之PCR-酶切試驗分類結果為依據，進行具識別性SCAR引子對的篩選，七個品系各6個單株材料先經PCR-酶切檢驗，各品系每個單株間並無差異性，而各品系的自交不親和基因型分類結果僅SP04與SP06為相同類型。而後進行花椰菜SCAR專一性引子的篩選，經PCR試驗結果後發現，有2組SCAR引子：D4-1*D7-2可鑑別品系SP01、SP04/SP06、SP08;K1-2*K4-4可鑑別品系SP07、SP08、SP09、SP10。c.番茄抗菸草嵌紋病毒分子標誌之建立：菸草嵌紋病毒為菸草鑲嵌病毒屬(Tobamovirus)，為番茄三大病毒之一。本試驗針對抗病基因(Tm-2)與病毒各開發專一性分子標誌(Tm2與TMV)。目前已開發一組共顯性分子標誌，可同時擴增番茄抗嵌紋病毒基因(Tm-2或Tm-2 2) 650 bp之DNA條帶、擴增感病基因(tm-2) 450 bp之DNA條帶。本研究與亞蔬中心合作開發一組分子標誌可檢測臺灣常見3種嵌紋病毒strains (TMV-0、TMV-1、TMV-2)，可擴增3.0 kb之DNA條帶。上述分子標誌可協助育種者早期篩選抗病植株、確認抗病基因型(R/R、R/S、S/S)及病毒感染情形，提高育種效率。 The projects of this program have three components including. The first is the establishment and application molecular markers of the resistance to Fusarium oxysporum in tomato analysis system. Specific markers I2 was developed for differentiating allelic variation at I-2 locus. It amplifies fragments from both resistant and susceptible tomato accessions with different molecular sizes that corresponding to the presence of I-2/I-2
計畫名稱_中文 I-2/i-2 and i-2/i-2 alleles. This study we also merged primer set I2/Fu as Multiplex-PCR through once PCR
計畫名稱_英文 detecting I-2
計畫編號_中文 i-2 andFusarium oxysporum simultaneously. Primer I2/Fu could amplify resistance genes I-20.7 or 0.8 kb
計畫編號_英文 susceptibility gene i-2 2.2 kb and Fusarium spp. 1.0 kb of DNA bands. The molecular markers could help breeders early screening and confirmation disease resistant plant genotypes
主辦單位_中文 helping to accelerate the breeding and promotion of disease-resistant tomato lines.Using seven cauliflower lines which are commonly used breeding parental as test material. To do SCAR primers screening based on Brace’s PCRrestriction classification test results that established in 1994. The result of PCR-restriction classification in addition to the line SP04 and SP06 are same self-incompatibility genotype
主辦單位_英文 the other are different.Then the SCAR makers screening results showed that there are two sets of SCAR primers: D4-1 * D7-2 can identify lines SP01
主辦人_中文 SP04/SP06
主辦人_英文 SP08; K1-2 * K4-4 can identify lines SP07
中文摘要 SP08
英文摘要 SP09