1. data.coa.gov.tw
  2. 種苗研發成果
  3. 18831830
column value
年度 2013 植物種苗研究團隊-作物種苗微體繁殖技術之開發與改進 The study of healthy seedling production by tissue culture 102農科-9.1.1-種-X2 102AS -9.1.1-SS-X2 繁殖技術課 Propagation Technology Section 陳哲仁 Jen-Ren Chen 本研究目的為探討綠竹(Bambusa oldhami)芽體形成，以1 mg/L BA＋1 mg/L kinetin處理之側芽培植體形成叢生芽體最高約為85.1%，培養3週後已有不定芽形成。而不定芽形成率則以添加1 mg/L BA＋3 mg/L kinetin最高約為96.2%。在不定芽誘導發根試驗結果顯示以1.0-2.0 mg/L NAA或IAA之發根率94.1-95.8% 為最佳，平均根長約有1.8cm。透氣式培養容器在組織培養對植物生長與發育是很重要因子，下一個研究為利用三種不同型式之培養容器器對丹蔘發根之影響，使用在MS基本培養基中培養之叢生芽體，培植體來自側芽，在發根培養基中，使用透氣式塑膠培養瓶提高發根率(98.2%)、葉長(5.2 cm)、根數(2.37)、根長(4.1 cm)及最長的根(5.8cm)。本試驗目的在於了解不同培養基成分對春石斛組織培養之芽體增殖階段和根系誘導階段瓶苗品質之影響。由試驗結果得知，增殖階段組培苗之株高、葉數、葉長、葉寬、芽體增殖數和褐化率皆受到培養基中MS培養基、馬鈴薯粉和蔗糖三種成分濃度以及其交感效應之顯著影響。發根階段則主要受到MS培養基、香蕉粉、馬鈴薯粉和蔗糖濃度四種成分之主效應及含NAA在內五種成分彼此間之交感效應所影響。而經分析，於增殖階段以全量MS培養基加上4g/L馬鈴薯粉和20g/L蔗糖之處理組合最佳，根系誘導階段則以1/2MS培養基加上0.1mg/L NAA、30g/L香蕉粉、4g/L馬鈴薯粉和20g/L蔗糖之處理組合最佳。種苗金皇一號石斛冷水萃取物可以活化小鼠肺巨噬細胞(RHS cells)且促進NO的釋放。 The objectives of the present study were to develop culture method for shoot formation in bamboo (Bambusa oldhami). Treatment of 1 mg/L BA＋1 mg/L kinetin was found best in multiple shoots induction from lateral bud explants
計畫名稱_中文 induction percentage reached 85.1%
計畫名稱_英文 shoots were formed after culture for 3 weeks. The highest percent shoot formation which is 96.2% was found in the treatment of 1 mg/L BA＋3 mg/L kinetin.In addition
計畫編號_中文 treatment of 1.0-2.0 mg/L NAA or IAA was found best in root formation from shoot
計畫編號_英文 formation percentage reached 94.1-95.8%
主辦單位_中文 the average roots Length formed per shoot were 1.8cm。Vented vessel is an important factor effective on plant growth and development in micropropagation. The next present study describes the effects of different vessel (plastic vented vessel
主辦單位_英文 glass flask sealing with aluminum foil
主辦人_中文 and glass flask sealing with Rubber stopper) on some rooting characters of Danshen (Salvia miltiorrhiza) using multiple shoots cultured on MS basal medium. Explants were lateral shoots. In rooting media with plastic vented vessel was more effective. Maximum percentage of rooting(98.2%)
主辦人_英文 leaf length (5.2 cm)
中文摘要 root number (2.37)
英文摘要 root length (4.1 cm) and longest root length (5.8cm) were observed in explants treated with plastic vented vessel.We study the effects of additives in vitro culture on quality of plantlets Dendrobium. The results indicated that using MS medium supplemented with 4 g/L potato powder and 20 g/L sucrose are optimal for shoot proliferation. Rooting medium of the best is using 1/2MS medium supplemented with 0.1 mg/L NAA