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| column | value |
|---|---|
| 年度 | 2012 作物種苗病害檢測、驗證及防治技術之開發與應用 Application and development of detection |
| 計畫名稱_中文 | certification and control of crop diseases 101農科-10.3.1-種-X1 101AS -10.3.1-SS-X1 繁殖技術課 Propagation Technology Section 袁雅芬 Ya-fen Yuan 本年度已取得15個南瓜品系共216個樣本進行白粉病原菌接種試驗；同時自不同地點收集白粉病原菌進行繁殖與保存；並建立南瓜對白粉病抗性之病害等級；目前接種的品系中，僅少數品種為可能較具抗白粉病特性之品種。以濃度為108CFU/ml的西瓜果斑病菌汙染乾淨的西瓜種子，發芽後種子置床於72穴穴盤中，並以不同編號的根圈微生物菌液，澆灌處理上述接種的西瓜幼苗，篩選出的拮抗細菌編號15-7、15-8、15-9及15-10可使西瓜幼苗存活率較對照組增加3倍，可做為生物農藥開發之用。委託廠商依序列合成之馬鈴薯病毒peptide經Western blotting 反應無法以商業生產之抗血清檢測，仍須以病毒鞘蛋白全長序列選殖，並誘導表現之重組蛋白生產血清較佳。胡瓜栽培期間以亞磷酸溶液處理，調查對露菌病防治之影響，由調查結果顯示，使用亞磷酸溶液500倍與1 |
| 計畫名稱_英文 | 000倍噴施皆具有減緩或抑制露菌病發生的效果，但以亞磷酸溶液500倍處理之效果優於亞磷酸溶液1 |
| 計畫編號_中文 | 000倍，且以連續噴施對抑制胡瓜露菌病發生的效果較為持久。而在使用窄域油300倍、500倍及苦棟油300、500倍對於葉蟎發生率的調查結果顯示，雖然以窄域油及苦棟油處理對於葉蟎發生率皆具有抑制的效果，但防治效果較為短暫。 Fifteen different pumpkin hybrid lines were screened to the powdery mildew -resistance which inoculum pathogen species. According the inoculum results showed that the virulence were different by the host hybrid lines. There were a few of hybrid lines resistant to the powdery mildew. We construct a grade of the powdery mildew –resistance in pumpkin.Watermelon seeds inoculated with 108CFU/ml Acidovorax avenae subsp.citrulli were planted in 72-cell plug after germination. The seedlings were poured with culturing media of different rhyzosphere bacterial. By this method 4 antagonist bacterial : No. 15-7 |
| 計畫編號_英文 | 15-8 |
| 主辦單位_中文 | 15-9 and 15-10 were screened. They made the survival rate of watermelon seedlings increasing 3 times than the control. These bacterial may be used to develop microbial pesticides.The amino acid sequences of potato viruses coat protein were analyzed by a contractor. However the synthesized peptides were not detected by western blotting with commercial antiserum anti PVS |
| 主辦單位_英文 | PVX |
| 主辦人_中文 | PVY. It is necessary to clon full-length coat protein genes of viruses. Then inducing expression of recombitant proteins for antiserum production is better.The cucumber treated with different concentrations phosphorous acid |
| 主辦人_英文 | investigated the effect for the downy mildew during cultivated period.Result showed that the different concentrations phosphorous acid was effective on the cucumber downy mildew. The phosphorous acid applied at 2g/l was better than at 1g/l |
| 中文摘要 | and the effect was better while applying continuously. The spider mites could be control by treated with the narrow range oil(2㏄/l |
| 英文摘要 | 1㏄/l) and China-berry oil(2㏄/l |