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Title 前瞻技術於環境領域之應用
Abstract 本計畫提出以介電泳分離晶片配合拉曼光譜的應用於環境樣本隱孢子蟲之檢測。結果顯示介電泳晶片偵測與分離隱孢子蟲之可行性。惟不同樣品隱孢子蟲之介電泳力可能與所處環境有關，因而使介電泳晶片分離條件之最佳化困難度提高。未來將已具有高濃度隱孢子蟲之樣品作測試，以利介電泳晶片分離條件最佳化之尋找。另外，本計畫並應用原子力顯微鏡量測隱孢子蟲之形狀特性以利介電泳晶片偵測與分離隱孢子蟲條件之最佳化。在隱孢子蟲拉曼光譜分析方法之建立方面，目前以福馬林固定之隱孢子蟲樣品進行偵測。研究結果與文獻之隱胞蟲圖譜對照可發現同在1525 cm-1附近有明顯的訊號存在，顯示未來應用拉曼光譜分析隱孢子蟲之可行性。
EngTitle Application of advanced technology on environmental engineering
EngAbstract This project applied advanced technology of dielectrophoresis and Raman spectrum in detecting Cryptosporidium in environmental samples. The results indicated that the possibility of using dielectrophoresis chips to isolate Cryptosporidium, but different environmental samples might need optimization of differentiation conditions. For the Raman spectrum methodology, the signal of 1525 cm-1 was observed in Cryptosporidium-enriched samples and this was also mentioned in the literature.
ProjectYear 093
SponsorOrg 環檢所
ExecutingOrg
PublicFullVersionURL http://epq.epa.gov.tw/project/FileDownload.aspx?fid=3098